The Use of Potassium Tellurite, Sodium Azide, and Acetic Acid in a Selective Medium for the Isolation of Listeria Monocytogenes

Gray, M. L.; Stafseth, H. J.; Thorp, F.

doi:10.1128/jb.59.3.443-444.1950

Cited by 34 publications

(5 citation statements)

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“…Since it is a gram-positive rod, potassium tellurite (8,13), nitrofuran compounds such as guanofuracin (5-nitro-2furfurylidene aminoguanidine hydrochloride) (7), furacin, and furadantin (17), chloramphenicol (7), salt (7), naladixic acid (2), amphotericin B (16), and polymixin B (9) have been incorporated as selective agents into plating media. However, with each of these reports have surfaced conflicting data concerning the efficacy of the selective component or isolation procedure.…”

Section: Discussionmentioning

confidence: 99%

Method for flow cytometric detection of Listeria monocytogenes in milk

Donnelly¹,

Baigent²

1986

Appl Environ Microbiol

199

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This report describes a method for the detection of Listeria monocytogenes in raw milk by flow cytometric analysis of fluorescently labeled bacterial populations. The use of immunofluorescence in combination with measures of DNA content by propidium iodide labeling and size by light scattering enabled specific identification of L. monocytogenes from Streptococcus faecalis, Streptococcus agalactiae, Streptococcus uberis, Staphylococcus epidermidis, and Staphylococcus hyicus. Additional specific resolution of L. monocytogenes populations was achieved through selective enrichment of raw milk in Listeria enrichment broth. These procedures should permit the rapid screening of milk and other food samples for L. monocytogenes and eliminate many of the shortcomings associated with conventional fluorescent-antibody procedures.

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Section: Discussionmentioning

confidence: 99%

Method for flow cytometric detection of Listeria monocytogenes in milk

Donnelly¹,

Baigent²

1986

Appl Environ Microbiol

199

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“…lMcroc-occi and streptococci also grow on this medium. However, micrococci have an intensely black center and pinkish-yellow periphery, while streptococci are smaller and pinkish-grey, with a dull surface (79,98,152).…”

Section: Monocytogenes the Methods Recommended Bymentioning

confidence: 99%

Listeria monocytogenes and listeric infections.

Gray¹,

Killinger²

1966

Bacteriological Reviews

531

309

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“…Four-to 6-week-old Arabidopsis plants with well-expanded rosettes that had not initiated inflorescence growth and did not MOLECULAR PLANT PATHOLOGY (2006) 7 (6), 579-592 © 2006 BLACKWELL PUBLISHING LTD exhibit signs of purple pigmentation were used. Pseudomonas syringae pv tomato DC 3000 (PstDC3000) (Dong et al, 1991;Whalen et al, 1991) was kindly provided by Dr R. Darby (University of Wales, Aberystwyth), and was cultured at 28 -30 °C on nutrient broth medium (Gray et al, 1950) containing 50 mg/mL rifampicin and used in infections according to Swanson et al (1988). Pelleted cells were washed twice with 10 mM MgCl 2 and resuspended in the same solution at a final concentration of 10 6 cfu/mL.…”

Section: Plant Infection and Monitoring Protocolsmentioning

confidence: 99%

Extensin over‐expression in Arabidopsis limits pathogen invasiveness

Guo

Shirsat²

2006

Molecular Plant Pathology

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SUMMARY The function of the cell wall protein extensin has been the subject of much speculation since it was first isolated over 40 years ago. In order to investigate the role of extensins in plant defence, we used the gain-of-function strategy to generate transgenic Arabidopsis plants over-expressing the EXT1 extensin gene. These were infected with the virulent bacterial pathogen Pseudomonas syringae DC3000 and symptom development was monitored. Lesions on the transgenics were on average five-fold smaller than those on the wild-type, did not increase in area over the time period of infection, accumulated a small bacterial load and showed very little chlorosis outside the lesion boundary. By contrast, lesions on the wild-type were large, spread to over 50% of the leaf area, continued to increase in size over the time course of the infection, accumulated a bacterial load 100-fold higher than that found in the transgenics, and showed a large chlorotic area outside the lesion boundary. SEM of lesions showed no evidence of bacteria at the lesion boundary in the extensin-over-expressing transgenics, whereas bacteria were always seen at the lesion boundary on the wild-type. Analysis of transgenics carrying an EXT1-GUS promoter-reporter fusion showed expression of GUS in a ring around the boundary of the lesion. Basal defences and signal transduction pathways involved in plant defence were not perturbed in the transgenics, as shown by the analysis of the expression of PR1 and PDF1.2 genes. These results show that extensin over-expression limits pathogen invasiveness.

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The Use of Potassium Tellurite, Sodium Azide, and Acetic Acid in a Selective Medium for the Isolation of Listeria Monocytogenes

Cited by 34 publications

References 0 publications

Method for flow cytometric detection of Listeria monocytogenes in milk

Method for flow cytometric detection of Listeria monocytogenes in milk

Listeria monocytogenes and listeric infections.

Extensin over‐expression in Arabidopsis limits pathogen invasiveness

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