The use of probiotics in medical practice

Mombelli, B.; Gismondo, Maria Rita

doi:10.1016/s0924-8579(00)00322-8

Cited by 131 publications

(89 citation statements)

References 49 publications

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“…However, the most commonly used are bacteria belonging to the genera Lactobacillus, the first and largest group of microorganisms to be regarded as probiotics (Mombelli & Gismondo, 2000;Wolfson, 1999) and Bifidobacterium. These bacteria are indigenous to the human GIT (Bielecka et al, 2002;Tannock, 2001).…”

Section: Common Probiotic Microorganismsmentioning

confidence: 99%

“…In probiotic therapy, these beneficial microorganisms are ingested and thereby introduced to the intestinal microflora intentionally. This results in high numbers of beneficial bacteria to participate in competition for nutrients with and starving off harmful bacteria (Mombelli & Gismondo, 2000). The probiotics take part in a number of positive health promoting activities in human physiology (Chen & Yao, 2002).…”

mentioning

confidence: 99%

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Probiotics - What They Are, Their Benefits and Challenges

Thantsha¹,

Mamvura²,

Booyens³

2012

New Advances in the Basic and Clinical Gastroenterology

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Section: Common Probiotic Microorganismsmentioning

confidence: 99%

mentioning

confidence: 99%

Probiotics - What They Are, Their Benefits and Challenges

Thantsha¹,

Mamvura²,

Booyens³

2012

New Advances in the Basic and Clinical Gastroenterology

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“…These microorganisms are thought to help prevent and/or lessen various disorders such as diarrhoea, lactose intolerance, inflammatory bowel disease, irritable bowel syndrome, hypercholesterolemia and food allergy [15,19]. They can also improve local immunity and exert a positive effect on carcinogenesis, although the corresponding mechanisms of action remain to be fully elucidated [25,31].…”

Section: Introductionmentioning

confidence: 99%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

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-Propionibacteria, which are largely used as Swiss-type cheese starters, also constitute promising human probiotics because of their beneficial effects on intestinal bifidobacteria and bacterial enzyme activities. To be efficient, they must survive acid stress imposed within the stomach and the surfactant effects of bile salts. We thus investigated the ability of Propionibacterium freudenreichii (strain Propiofidus ® SI41) to adapt to, and survive these stresses. In an in vivo study, 7 volunteers received Propiofidus ® SI41 in 3 different modes. The probiotic was supplied during 3 periods (i) in classical capsules at a low dose (5 × 10 9 CFU·d -1 ), (ii) in the same capsules at a higher dose (5 × 10 10 CFU·d -1 ), (iii) in acid-resistant capsules at the low dose. In the first case, propionibacteria were recovered above 10 5 CFU·g -1 in only 8 out of 14 faecal samples. In the two following cases, they were enumerated in all faecal samples between 10 5 and 10 7 CFU·g -1 . The recovery of live propionibacteria in faecal samples seemed to be related to a higher content in short chain fatty acids. This study, which clearly showed the role of gastric protection in the survival of propionibacteria, led us to investigate stress adaptation in this strain. Acid tolerance response was evidenced: transient exposure to pH 5 afforded protection towards acid challenge at pH 2. Moreover, we showed that pre-exposure to a moderate bile salts pre-treatment at 0.2 g·L -1 sharply increased its survival against a subsequent lethal challenge (1 g·L -1 ). Important changes in cellular protein synthesis were observed during adaptation using two-dimensional electrophoresis. Global protein synthesis decreased during adaptation treatments. In contrast, specific proteins were overexpressed during acid adaptation and others during bile salts adaptation. Among the up-regulated polypeptides, a carboxyl carrier protein (BCCP) and the recombinase RecR were identified during both stress responses while the universal chaperonins GroEL and GroES, as well as the replicase RepB, were only up-regulated during acid adaptation. These results bring new insights on the tolerance of P. freudenreichii to acid and bile salts, which should be taken into consideration for the development of probiotic preparations. Résumé -Survie et effets bénéfiques des bactéries propioniques dans l'intestin humain : étu-des in vivo et in vitro. Les propionibactéries, qui sont largement utilisées comme levain d'affinage des fromages à pâte pressée cuite, constituent également des probiotiques humains prometteurs du fait de leurs effets bénéfiques sur la flore bifide et les activités enzymatiques bactériennes, au niveau de l'intestin. Pour être efficaces, elles doivent survivre au stress acide subi dans l'estomac et à l'action détergente des sels biliaires. Nous avons donc étudié la capacité de Propionibacterium freudenreichii (souche Propiofidus ® SI41) à s'adapter et à survivre à ces stress. Dans une étude in vivo, 7 volontaires ont reçu Propiofidus ® SI41 selon 3 mo...

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“…The significant effects of probiotics on their hosts" health status are achieved when an adequate concentration of the bacteria is present in the environment which varies with the species [8]. Likewise, the probiotic properties may be affected by environmental characteristics of the host"s gastrointestinal tract.…”

Section: Introductionmentioning

confidence: 99%