The use of proTeomic Technologies in breasT cancer research

Abstract

The main findings in the field of breast cancer proteomic research as well as modern strategies, technologies and methods of validation are reviewed. A special attention is focused on validated proteomic biomarkers of breast cancer. The data on proteomic profiling of stroma, tumor microenvironment, involvement of proteins in tumor progression, invasion and metastasis, and mechanisms of action of new generation drugs, are analyzed. The results of proteomic analysis are of high clinical importance and significantly improve tumor molecular profiling, stratification of patients, screening, diagnostics, and therapy of breast cancer.

Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt

“…Different proteomic technologies have identified unique proteome patterns that can diagnose BC in different clinical stages and recognize different outcome and response to therapy (Mazur and Pyatchanina, 2016). Furthermore, reproducibility of MS based protein profiles for diagnosis of BC across clinical studies has been demonstrated in systematic review (Callesen et al, 2008).…”

“…Mass spectrometry outperformed other techniques in its ability to detect hundreds of proteins in a sole experiment. These proteins are either up or down regulated when comparing the proteomic profiling between malignant and non malignant biological fluids (Mazur and Pyatchanina, 2016;Yang et al, 2016).…”

Use of Lectin-based Affinity Techniques in Breast Cancer Glycoproteomics: A Review

“…Proteomics allows monitoring of the changes in the relative amount and the distribution of proteins that are encoded by cancer genes in tumor tissues or compares the expression of proteins in tumors and corresponding normal tissue. Thus modern proteomic technologies, utilizing gel electrophoresis (1D- or 2D-PAGE and 2D-DIGE) and liquid chromatography (LC) combined with MS, have recently been increasingly applied to the study of carcinoma. − The use of LC–MS/MS together with quantitative methods, such as, isotope-coded affinity tagging (ICAT–LC–MS/MS), isobaric tagging for relative and absolute quantitation (iTRAQ–LC–MS/MS), or stable isotope labeling with amino acids in cell cultures (SILAC–LC–MS/MS), is promising for the discovery of new cancer protein biomarkers …”

“…The steeply growing number of publications related to cancer proteomics and glycoproteomics proves that this is a hot topic (Figure ). A summary of proteomic approaches, advancements, and their applications for profiling analyses of tumor tissues/cell has been published in various reviews, but only a few of them are especially focused on breast cancer. ,,,− …”