The use of proteomics to study wound healing: a preliminary study for forensic estimation of wound age

Tarran, Sarah; Craft, George E.; Valova, Valentina A.; Robinson, Phillip J.; Thomas, Graham; Markham, Robert; Langlois, Neil E. I.; Vanezis, Peter

doi:10.1258/rsmmsl.47.2.134

Cited by 13 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of MALDI-ToF MS to incised wounds of rats demonstrates that, after wounding, the most prominent change is in the level of haemoglobin, which is elevated after 5 min and remains elevated for 3 h, falling to near control levels after 12 h [92].…”

Section: Geno-proteomics and Future Perspectivesmentioning

confidence: 99%

Estimating wound age: looking into the future

Cecchi

2010

Int J Legal Med

106

View full text Add to dashboard Cite

show abstract

Section: Geno-proteomics and Future Perspectivesmentioning

confidence: 99%

Estimating wound age: looking into the future

Cecchi

2010

Int J Legal Med

106

View full text Add to dashboard Cite

show abstract

“…Based on the small body of reported studies, proteomics analyses have been performed on both wound fluids and biopsied tissues. One such study 38 reported changes in hemoglobin level with time in tissues from experimentally inflicted skin wounds on rats. Oh et al…”

Section: Discussion Of Relevant Literaturementioning

confidence: 99%

Bottom-Up Mass Spectrometry–Based Proteomics as an Investigative Analytical Tool for Discovery and Quantification of Proteins in Biological Samples

Amunugama

Jones

Ford

et al. 2013

Advances in Wound Care

View full text Add to dashboard Cite

Objective: The objective of this overview is to introduce bottom-up mass spectrometry (MS)-based proteomics approaches and strategies, widely used in other biomedical research fields, to the wound-healing research community. Approaches: Two major proteomics workflows are discussed: gel-based and gel-free chromatographic separation to reduce the complexity of the sample at protein and peptide level, respectively, prior to nano-liquid chromatographytandem mass spectrometry analysis. Other strategies to discover less abundant proteins present in the sample, are also briefly discussed along with label-free and label-incorporated methods for protein quantification. Overall, the experimental workflows are designed and continually improved to increase the number of proteins identifiable and quantifiable. Discussion: Recent advances and improvements in all areas of proteomics workflow from sample preparation, to acquisition of massive amounts of data, to bioinformatics analysis have made this technology an indispensable tool for in-depth large-scale characterization of complex proteomes. This technology has been successfully applied in studies focusing on biomarker discovery, differential protein expression, protein-protein interactions, and post-translational modifications in complex biological samples such as cerebrospinal fluid, serum and plasma, and urine from patients. The publications from these studies have reported greater number of identified proteins, novel biomarker candidates, and post-translational modifications previously unknown. Conclusions: The qualitative and quantitative protein analysis of the protein population of wound tissues or fluids at different stages is important in wound healing research. Given the complexities and analytical challenges of these samples, MS-based proteomic workflows further improved with recent advances offer a powerful and attractive technology for this purpose.

show abstract

“…Subjects enrolled in the study were seen on days 0, 1, 2, 3,4,7,8,9,10,11,14,21,28,35 and 42. On each visit, the wounds were digitally photographed and sampled as previously reported on both the periphery and interior of the wound (3).…”

Section: Protocolmentioning

confidence: 99%

Analysis of pressure ulcer wound fluid using two‐dimensional electrophoresis

Wyffels

Fries

Randall

et al. 2010

International Wound Journal

View full text Add to dashboard Cite

The incidence rate of pressure ulcers in the USA ranges from 0.4% to 38% in acute care settings and from 2.2% to 23.9% in long-term care settings, and their treatment costs are in the billions of dollars yearly. The proteome of wound fluid may contain early indicators or biomarkers associated with healing in pressure ulcers that would enable treatment regimes to be optimised for each individual. Wound fluid was collected from the interior and periphery of 19 chronic pressure ulcers at 15 time points during 42 days for an analysis of protein expression. Proteins were fractionated using two-dimensional polyacrylamide gel electrophoresis. A comparison of the spot distributions indicates a biochemical difference between the interior and the periphery of wounds. Pressure ulcers that healed show a greater number of spots for interior and peripheral locations combined over time when compared with wounds that did not heal. Using this technique, protein S100A9 was identified as a potential biomarker of wound healing. The identification of differences within the proteome of healing versus non healing pressure ulcers could have great significance in the use of current treatments, as well as the development of new therapeutic interventions.

show abstract

The use of proteomics to study wound healing: a preliminary study for forensic estimation of wound age

Cited by 13 publications

References 36 publications

Estimating wound age: looking into the future

Estimating wound age: looking into the future

Bottom-Up Mass Spectrometry–Based Proteomics as an Investigative Analytical Tool for Discovery and Quantification of Proteins in Biological Samples

Analysis of pressure ulcer wound fluid using two‐dimensional electrophoresis

Contact Info

Product

Resources

About