The use of radioiodinated human serum albumin in measurements of arterial permeability

Winlove, C. Peter; Davis, Jane; Rubin, B.T.; Dell, Ralph B.

doi:10.1093/cvr/12.10.609

Cited by 9 publications

(3 citation statements)

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“…Fifteen minutes before the animals were killed, each rabbit was injected intravenously with 6 ml of a 0.5% Evans blue dye solution, which stained areas of deendothelialized aorta blue. 6 Immediately after each rabbit was killed, the aorta was removed and rinsed thoroughly with saline to remove any free radioactive iodide 28 and radioactive blood. The adventitia was gently stripped away and the aorta opened along the ventral surface.…”

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Time course of 125I-labeled LDL accumulation in the healing, balloon-deendothelialized rabbit aorta.

Chang

Lees

1992

Arterioscler Thromb

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We previously showed by qualitative en face autoradiography that after 24 hours of circulation, 12S I-labeled low density lipoprotein (LDL) injected in tracer amounts accumulated focally at the edges of regenerating endothelial islands in the balloon catheter-deendothelialized aorta of the normocholesterolemic rabbit In the present study with the same animal model, we have used quantitative autoradiography to examine li5 I-LDL accumulation in the healing aorta as a function of LDL circulation time from 2.5 to 40 hours. The results demonstrated that '"I-LDL accumulation in the healing aorta occurred in two kinetically and biochemically distinct compartments, one of which was in equilibrium with plasma and one of which sequestered LDL. LDL accumulation in the still-deendothelialized aorta (DEA) was diffuse and only moderately intense on autoradiography. It peaked 4 hours after injection; over the following 36 hours the disappearance of IZ5 I-LDL from DEA paralleled the disappearance of IU

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confidence: 99%

Time course of 125I-labeled LDL accumulation in the healing, balloon-deendothelialized rabbit aorta.

Chang

Lees

1992

Arterioscler Thromb

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“…One isolated report, for example, describes a direct relation between flux of cholesterol into the aorta of alloxan-diabetic rats and serum cholesterol or LDL concentration that does not differ from that of normal animals (Matsuda and Kalant, 1966); when the circulating level increases, flux increases. Not only are available data of this type extremely limited, but studies of flux of labeled proteins, such as albumin, are plagued with methodological problems (Winlove et al, 1978). deDuve (1974) has calculated that maximal acid lipolytic activity by cells in the rabbit aorta exceeds by only a factor of two the calculated rates of cholesterol ester influx into aortas from severely hypercholesterolemic animals (Newman and Zilversmit, 1966), but this is acknowledged to be a crude approximation.…”

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confidence: 99%

A proposal linking clearance of circulating lipoproteins to tissue metabolic activity as a basis for understanding atherogenesis.

Wolinsky¹

1980

Circulation Research

143

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“…In the study of vascular permeability, perfusion of exogenous albumin tagged to different markers such as colloidal gold [19,20,28,46], isotopes [1,2,8,16], fluorescent tracers [24,27], and other dyes [7,48], has been extensively applied. The use of such exogenous tracers, though extremely valuable, is confronted by major limitations [3,33,43,47]. The detection of endogenous albumin through the immunocytochemical approach [5,6,26,33,36,37], allows for the in situ localization of the circulating protein in steady-state conditions, circumventing problems inherent to the use of exogenous tracers.…”

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Quantitative immunocytochemical studies of endogenous albumin in rat aortic endothelial and mesothelial cells

Londono¹,

Bendayan²

1990

Biology of the Cell

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Endogenous albumin was revealed over cellular structures of rat ascendent aorta endothelia and mesothelium, with high resolution and specificity, by applying the protein A-gold immunocytochemical approach. This approach allows albumin distribution to be studied under steady-state conditions. The cellular layers evaluated were the aortic endothelium, the capillary endothelium (vasa vasorum), and the mesothelium externally lining the aorta at this level. Gold particles, revealing albumin antigenic sites, were preferentially located over plasmalemmal vesicles and intercellular clefts of endothelial and mesothelial cells, though with different labeling intensities. The interstitial space was also labeled. Morphometrical evaluation of plasmalemmal vesicles demonstrated a higher surface density for these structures in capillary endothelial cells (12%) compared with those in aortic endothelial (5%) and mesothelial cells (2%). Quantitation of gold labeling intensities over these structures revealed a higher labeling over plasmalemmal vesicles of capillary endothelium than over those of aortic endothelium and mesothelium. This result, together with the higher surface density of plasmalemmal vesicles found in capillary endothelium, suggest an important role of these structures in the transendothelial passage of endogenous albumin, particularly for capillary endothelium. On the other hand, labeling densities over mesothelial clefts were found to be higher than those of capillary and aortic endothelia. Results from this study concur with the proposal of a differential passage of albumin according to the cell lining considered, and suggest to a role for mesothelial intercellular clefts in contributing to the presence of albumin in interstitial spaces.

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The use of radioiodinated human serum albumin in measurements of arterial permeability

Abstract: The interactions of radioiodinated human serum albumin and sodium iodide with plasma proteins and their uptake by the arterial wall are investigated in vivo and in vitro. The results clearly show that in most situations iodinated albumin is unsuitable for studies on arterial permeability.

Cited by 9 publications

References 0 publications

Time course of 125I-labeled LDL accumulation in the healing, balloon-deendothelialized rabbit aorta.

Time course of 125I-labeled LDL accumulation in the healing, balloon-deendothelialized rabbit aorta.

A proposal linking clearance of circulating lipoproteins to tissue metabolic activity as a basis for understanding atherogenesis.

Quantitative immunocytochemical studies of endogenous albumin in rat aortic endothelial and mesothelial cells

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