The use of regenerated immunoaffinity columns for aflatoxins B1, B2, G1 and G2 in peanut confection

Iha, Maria Helena; Mini, Camila Alessandra; Okada, Isaura Akemi; Briganti, Rita de Cássia; Trucksess, Mary W

doi:10.1016/j.chroma.2016.12.040

Cited by 21 publications

(4 citation statements)

References 12 publications

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“…The limits of detection (LOD), based on 3 times the signal-to-noise ratio, were in the range 0.003–0.007 μg·kg –1 , and the limits of quantification (LOQ), based on 10 times the signal-to-noise ratio, were in the range 0.009–0.023 μg·kg –1 . Thus, the nanoarchitecture developed here is about 10-fold more sensitive than the IAC and SPE methods.…”

Section: Resultsmentioning

confidence: 91%

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Design of Multifunctional Nanostructure for Ultrafast Extraction and Purification of Aflatoxins in Foodstuffs

et al. 2017

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Aflatoxins (AFs) are a class of carcinogens, associated with liver cancers, that exist in foodstuffs. There are extremely low maximum limits of AFs in foodstuffs (0.025-20 μg·kg). Quick and sensitive detection of such low concentration of AFs in foodstuffs is dominated by the efficiency and selectivity of the AF enrichment process, which is extremely challenging although substantial efforts have been made in recent decades. Here we design and synthesize a multilayer nanoarchitecture composed of a broad-spectrum aflatoxin monoclonal antibody shell, chitosan middle layer, and magnetic bead core (denoted AF-mAb/CTS/FeO). The efficiency of AF-mAb/CTS/FeO in extracting AFs has been found to be more than 60 times higher than both conventional immunoaffinity chromatography and solid-phase extraction. Furthermore, the nanocomposite displays excellent selectivity and good reusability as well as outstanding efficiency. When coupled to ultraperformance liquid chromatography-tandem quadrupole mass spectrometry, this new nanoarchitecture enables us to probe six AFs at concentrations as low as 0.003 μg·kg in foodstuffs with free matrix effects, which is nearly 10 times smaller than the regulated maximum tolerated does. It is believed that the new nanoarchitecture will provide an efficient and fast pathway to detect AFs in foodstuffs to protect human being from some critical liver cancers.

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Section: Resultsmentioning

confidence: 91%

“…Thus, the nanoarchiteture developed here is about 10-fold more sensitive than the IAC 43 and SPE methods. 44 Applications.…”

Section: N-hydroxysulfosuccinimidementioning

confidence: 91%

Design of Multifunctional Nanostructure for Ultrafast Extraction and Purification of Aflatoxins in Foodstuffs

et al. 2017

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“…Aptamers are oligonucleotide compounds comprised of 20–100 nucleotides with a complex tertiary or quaternary structure [ 24 ]. Aptamers have been developed that can detect targets in the micromolar range, which is comparable to antibodies.…”

Section: Introductionmentioning

confidence: 99%

Determination of Aflatoxin B1 in Grains by Aptamer Affinity Column Enrichment and Purification Coupled with High Performance Liquid Chromatography Detection

Ji,

Sun,

Fang

et al. 2024

Foods

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Aflatoxin B1 (AFB1) is a highly teratogenic and carcinogenic secondary metabolite produced by Aspergillus. It is commonly detected in agricultural products such as cereals, peanuts, corn, and feed. Grains have a complex composition. These complex components severely interfere with the effective extraction and separation of AFB1, and also cause problems such as matrix interference and instrument damage, thus posing a great challenge in the accurate analysis of AFB1. In this study, an aptamer affinity column for AFB1 analysis (AFB1-AAC) was prepared for the enrichment and purification of AFB1 from grain samples. AFB1-AAC with an AFB1-specific aptamer as the recognition element exhibited high affinity and specificity for AFB1. Grain samples were enriched and purified by AFB1-AAC, and subsequently analyzed by high performance liquid chromatography with post-column photochemical derivatization-fluorescence detection (HPLC-PCD-FLD). The average recoveries of AFB1 ranged from 88.7% to 99.1%, with relative standard deviations (RSDs) of 1.4–5.6% (n = 3) at the spiked levels of 5.0–20.0 μg kg−1. The limit of detection (LOD) for AFB1 (0.02 μg kg−1) was much below the maximum residue limits (MRLs) for AFB1. This novel method can be applied to the determination of AFB1 residues in peanut, corn, and rice.

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“…Rhemrev et al [ 20 ] developed a new type of reusable immunoaffinity cassette that could be used for 15 times for fully-automated aflatoxin analysis with satisfactory recovery rates. Iha et al [ 21 ] worked on the regeneration of IACs for aflatoxins that could be utilized up to nine times in peanut confection. Obviously, these reported studies are mainly focused on food matrices and CHMs were not involved.…”

Section: Introductionmentioning

confidence: 99%

Regeneration and Reuse of Immunoaffinity Column for Highly Efficient Clean-Up and Economic Detection of Ochratoxin A in Malt and Ginger

Liu

Huang

et al. 2018

Toxins

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Immunoaffinity columns (IACs) are most popularly used for mycotoxin clean-up in complex matrices prior to chromatographic analysis. But, their high cost has limited their wide application and the regeneration of IACs for multiple instances of reuse is important. This study aimed to investigate the feasibility of regeneration and reuse of IACs for purification of ochratoxin A (OTA) in spiked raw malt and dried ginger samples followed by high performance liquid chromatography-fluorescence detection. After each use, the IACs were filled with phosphate buffer saline (PBS) as the preservation solution and stored at 8 °C overnight for regeneration and reuse until the recovery rate was <70%. The results showed that matrix type, preparation procedure, and pH value of sample extraction exhibited major effects on the reuse of IACs for OTA clean-up. While, after modifying the sample preparation procedure using water as the diluent and the solution at a pH of 7 to 8, the IACs could be used eight and three times for the spiked raw malt and dried ginger samples with OTA after regeneration. Regarding the traditional procedure recommended in Chinese Pharmacopoeia (2015 edition), the IACs could be used for three and two times for the spiked raw malt and dried ginger samples with OTA, respectively. Therefore, the corresponding experimental cost could be reduced to one-eighth and one-third of the original cost. This is the first study on the regeneration and reuse of IACs for OTA clean-up in complex Chinese herbal medicines, providing a green and economical tool for a large number of samples analysis with low cost.

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The use of regenerated immunoaffinity columns for aflatoxins B1, B2, G1 and G2 in peanut confection

Cited by 21 publications

References 12 publications

Design of Multifunctional Nanostructure for Ultrafast Extraction and Purification of Aflatoxins in Foodstuffs

Design of Multifunctional Nanostructure for Ultrafast Extraction and Purification of Aflatoxins in Foodstuffs

Determination of Aflatoxin B1 in Grains by Aptamer Affinity Column Enrichment and Purification Coupled with High Performance Liquid Chromatography Detection

Regeneration and Reuse of Immunoaffinity Column for Highly Efficient Clean-Up and Economic Detection of Ochratoxin A in Malt and Ginger

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