The Use of Regenerating Bone Marrow to Protect Guinea-Pigs against Lethal Irradiation

Harris, Paul J.; Kugler, J. H.

doi:10.1159/000209566

Cited by 13 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animals were irradiated with a 200 kv machine delivering 38.6 R/rain at a target distance of 45.5 cm using 1.0 mm aluminum and 0.05 mm copper filters (HVL, half value layer, 1.3 ram Cu; F-0.94). In animals not protected with bone marrow cells, this dose proved lethal (see Table I), as had been shown previously (17).…”

Section: Mantels ¢~ Methodssupporting

confidence: 81%

TRANSFER OF RESPONSIVENESS TO HAPTEN CONJUGATES OF POLY-L-LYSINE AND OF A COPOLYMER OF L-GLUTAMIC ACID AND L-LYSINE TO LETHALLY IRRADIATED NON-RESPONDER GUINEA PIGS BY BONE MARROW OR LYMPH NODE AND SPLEEN CELLS FROM RESPONDER GUINEA PIGS

Foerster

Green

Lamelin

et al. 1969

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Hartley guinea pigs genetically unresponsive to hapten-PLL (poly-L-lysine) conjugates were lethally irradiated and given allogeneic bone marrow from Hartley responder animals. Many of the animals died of graft versus host disease before their response to 2,4-dinitrophenyl-PLL (DNP-PLL) could be measured. The immune response of the surviving recipient animals was evaluated by anti-DNP antibody production, development of delayed hypersensitivity to DNP-poly-L-lysine, as well as by lymph node cell stimulation in vitro by this antigen. 12 of 14 recipient animals thus treated made an immune response as measured by 2 of the 3 parameters. Strain 13 guinea pigs, genetically unable to respond immunologically to DNP-PLL and to DNP-GL (2,4-dinitrophenyl-L-glutamic acid L-lysine copolymer) were lethally irradiated and given bone marrow from (2 x 13) F1 responder animals or strain 13 bone marrow and (2 x 13) F1 lymph node and spleen cells. A high proportion of the animals survived this procedure; no evidence of graft versus host disease was observed. Three of three strain 13 animals irradiated and, given strain 13 bone marrow and (2 x 13) F1 lymph node and spleen, and then immunized with DNP-PL, made a specific immune response. 7 of 10 irradiated strain 13 animals given strain 13 bone marrow and (2 x 13) F1 lymph node and spleen made an immune response to DNP-GL. However, only one of six irradiated strain 13 animals made a vigorous immune response to DNP-GL after reconstitution with (2 x 13) F1 bone marrow alone. The ability to transfer the immune response to PLL antigens from responder to nonresponder animals demonstrates unequivocally that the defect in the non-responder animals is immunological rather than due to some other type of non-immunological mechanism. The bone marrow contains all the immunological cells necessary for the expression of the PLL gene. However, the finding that (2 x 13) F1 lymph node and spleen cells were more effective than (2 x 13)F1 bone marrow cell populations (known to be a rich source of monocyte precursors) suggests that the cells in which the PLL gene function is expressed may be lymphocytes rather than monocytes and macrophages.

show abstract

Section: Mantels ¢~ Methodssupporting

confidence: 81%

TRANSFER OF RESPONSIVENESS TO HAPTEN CONJUGATES OF POLY-L-LYSINE AND OF A COPOLYMER OF L-GLUTAMIC ACID AND L-LYSINE TO LETHALLY IRRADIATED NON-RESPONDER GUINEA PIGS BY BONE MARROW OR LYMPH NODE AND SPLEEN CELLS FROM RESPONDER GUINEA PIGS

Foerster

Green

Lamelin

et al. 1969

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…There is no agreement as yet on the identity of the undifferentiated erythroid precursor cell2, although cells morphologically of the lymphocytic series have been attributed this function on the basis of indirect evidence [1][2][3][4][5][6][7]. In an attempt to gain further information about the identity of precursors to erythroblasts, the effects of erythro poietic stimulation were studied in bone marrow in which erythropoiesis had previously been depressed.…”

mentioning

confidence: 99%

Identity of Erythroblast Precursors in Bone Marrow

et al. 1970

View full text Add to dashboard Cite

Effects of Anaemia on DNA‐Synthesizing Cells in Peripheral Blood and Observations on Their Origin

Harris

1973

Novartis Foundation Symposia

View full text Add to dashboard Cite

The Use of Regenerating Bone Marrow to Protect Guinea-Pigs against Lethal Irradiation

Cited by 13 publications

References 26 publications

TRANSFER OF RESPONSIVENESS TO HAPTEN CONJUGATES OF POLY-L-LYSINE AND OF A COPOLYMER OF L-GLUTAMIC ACID AND L-LYSINE TO LETHALLY IRRADIATED NON-RESPONDER GUINEA PIGS BY BONE MARROW OR LYMPH NODE AND SPLEEN CELLS FROM RESPONDER GUINEA PIGS

TRANSFER OF RESPONSIVENESS TO HAPTEN CONJUGATES OF POLY-L-LYSINE AND OF A COPOLYMER OF L-GLUTAMIC ACID AND L-LYSINE TO LETHALLY IRRADIATED NON-RESPONDER GUINEA PIGS BY BONE MARROW OR LYMPH NODE AND SPLEEN CELLS FROM RESPONDER GUINEA PIGS

Identity of Erythroblast Precursors in Bone Marrow

Effects of Anaemia on DNA‐Synthesizing Cells in Peripheral Blood and Observations on Their Origin

Contact Info

Product

Resources

About