The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations

Baker, Andrea; Schroeder, Declan C.

doi:10.1186/1743-422x-5-10

Cited by 52 publications

(54 citation statements)

References 30 publications

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“…ABPV has been assigned to the family Dicistroviridae and is known to follow a classic acute-type infection strategy since relatively low loads (10 3 to 10 6 viruses per honeybee) can rapidly translate into overt symptoms of paralysis and ultimately death for the honeybee, depending on the mode of transmission (6,33). ABPV shares Ͼ92% sequence homology with other members of the family Dicistroviridae, Kashmir bee virus and Israeli acute paralysis virus, across the eight conserved domains of the RNA-dependent RNA polymerase gene, and it has been proposed that these viruses have recently diverged and are variants of each other (7). Advances in the study of this proposed ABPV complex is revealing the significant impact these viruses may have on honeybee colonies on a global scale.…”

mentioning

confidence: 99%

Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses

Highfield

Nagar

Mackinder

et al. 2009

Appl Environ Microbiol

267

240

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The worldwide decline in honeybee colonies during the past 50 years has often been linked to the spread of the parasitic mite Varroa destructor and its interaction with certain honeybee viruses. Recently in the United States, dramatic honeybee losses (colony collapse disorder) have been reported; however, there remains no clear explanation for these colony losses, with parasitic mites, viruses, bacteria, and fungal diseases all being proposed as possible candidates. Common characteristics that most failing colonies share is a lack of overt disease symptoms and the disappearance of workers from what appears to be normally functioning colonies. In this study, we used quantitative PCR to monitor the presence of three honeybee viruses, deformed wing virus (DWV), acute bee paralysis virus (ABPV), and black queen cell virus (BQCV), during a 1-year period in 15 asymptomatic, varroa mite-positive honeybee colonies in Southern England, and 3 asymptomatic colonies confirmed to be varroa mite free. All colonies with varroa mites underwent control treatments to ensure that mite populations remained low throughout the study. Despite this, multiple virus infections were detected, yet a significant correlation was observed only between DWV viral load and overwintering colony losses. The long-held view has been that DWV is relatively harmless to the overall health status of honeybee colonies unless it is in association with severe varroa mite infestations. Our findings suggest that DWV can potentially act independently of varroa mites to bring about colony losses. Therefore, DWV may be a major factor in overwintering colony losses.

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mentioning

confidence: 99%

Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses

Highfield

Nagar

Mackinder

et al. 2009

Appl Environ Microbiol

267

240

View full text Add to dashboard Cite

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“…Hence, the 3 0 region of our primers must anneal at highly conserved regions and especially the 3 0 terminus must be most distant from the most variable 3rd nucleotide of a codon (de Miranda, 2008). The 3 0 terminus of the forward primers anneals at the 2nd nucleotide of the D codon of the conserved motif KD in domain I of the RNA-dependent RNA polymerase (RdRp) of Picornavirales (Lanzi et al 2006;Baker and Schroeder, 2008b) and DTLKD in honeybee dicistroviruses and iflaviruses. The 3 0 terminus of the reverse primers of KBV, ABPV and IAPV anneals at the first nucleotide of the G codon of the conserved motif SGXXXT in Picornavirales and PSGNPAT in honeybee dicistroviruses (except for Black queen cell virus (BQCV) PSGXXXT), which is located in domain V of the RdRp.…”

Section: Resultsmentioning

confidence: 99%

“…Primers described to detect KBV (Stoltz et al, 1995) probably amplified certain variants of Israeli acute paralysis virus (IAPV) (Blanchard et al, 2008;Palacios et al, 2008). The primer sets for ABPV detection of Tentcheva et al (2004) and Baker and Schroeder (2008a) can theoretically amplify IAPV (Baker and Schroeder, 2008b). Hence, primer design for diagnostic purpose of highly mutant Picornavirales faces an ambiguity since too conserved regions result in unspecific primers.…”

Section: Introductionmentioning

confidence: 98%

Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees

Meeus

Smagghe

Siede

et al. 2010

Journal of Invertebrate Pathology

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“…Morphologically, these viruses, with the exception of CBPV, are very similar, showing isometric shaped protein capsids of approximately 20-30 nm in diameter and non-occluded (Bailey et al, 1976;Chen et al, 2005b;Baker and Schroeder, 2008). The outer part of the capsid comprises of 60 repeated protomers, each one con- S30-47, with permission from Elsevier sisting of a single molecule of three subunits: VP1, VP2, and VP3.…”

Section: Viruses In Honey Beesmentioning

confidence: 99%

Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

Matusiewicz¹,

Margas²,

Mazurek³

et al. 2016

bta

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Honey bees (Apis mellifera ) play a crucial function in terrestrial ecosystems by maintaining floral biodiversity and providing sustainable agriculture through pollination. Bees affect 35% of the world's crop production, hence, they are essential for economic sustainability, agriculture and food security. The economic value of pollination by bees goes beyond agricultural production. An important role of bees is pollination of the plants that provide food to the wild animals, and this is part of the proper functioning of the ecosystem. On the one hand Apis mellifera has great adaptive potential and it may be found in highly diverse climates almost everywhere in the world, but on the other hand bees are under constant threat by numerous pathogens including bacteria, viruses, fungi, Microsporidia, mites and insects. Other stress factors include environmental change-related stresses, pesticides, malnutrition and migratory beekeeping. The interactions among these, along with possibly other unknown factors, are causing widespread decline in honey bee populations -Colony Collapse Disorder (CCD) syndrome. Viruses are the most recent and still poorly investigated pathogens of the honey bees. Simultaneously, Varroa destructor mites and viruses are currently the high-profile suspects in collapsing bee colonies. Varroa acts as a vector for a range of RNA viruses of honey bees which are transferred when the mite feeds on hemolymph from the developing pupa. This publication aims to present an overview of the most important honey bee viruses, their diagnostics methods and treatment opportunities with emphasis on the most common and devastating viruses from Dicistroviridae and Iflaviridae families.

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The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations

Cited by 52 publications

References 30 publications

Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses

Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses

Multiplex RT-PCR with broad-range primers and an exogenous internal amplification control for the detection of honeybee viruses in bumblebees

Viral infections of Apis mellifera : its importance, dynamics, diagnostics and treatment opportunities

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