The Use of Salacca Zalacca Extract as Reducing Agent to Synthesize Silver Nanoparticles (Agnps) and the Antibacterial Activities.

Abstract

In general, leaf, flower, and fruit of plants contain organic compounds which may reduce metal salts to nanosize metals, or nanoparticles (NPs). The extract of snake fruit (Salacca zallaca) has been used to synthesize silver nanoparticles (AgNPs) using green-one pot method. Some reaction variables ie. incubation time, Salacca zallacca extract concentration, and pH of the mixture of AgNO3 and the extract have been set-up and controlled. The AgNPs were characterized using UV-Vis, FTIR, XRD, and TEM methods. Testing the antibacterial activity of AgNPs was undertaken using the disk diffusion method by measuring the inhibition zones of Staphylococcus aureus and Salmonella typhi bacteria.The AgNPs can be prepared by reducing Ag+ using Salacca zalacca extract as reducing agent. The formation of AgNPs are affected by the incubation time, extract concentration, and pH of the solution. The AgNPs indicate SPR peak absorption at 400-530 nm, and centered cubic (fcc) structures with a particle size of 10 nm. The AgNPs show antibacterial activity against Staphylococcus aureus and Salmonella typhi bacteria.