The use of scanning ion conductance microscopy to image A6 cells

Gorelik, Julia; Zhang, Yanjun; Shevchuk, Andrew; Frolenkov, Gregory I.; Sánchez, Daniel; Lab, Max J.; Vodyanoy, Igor; Edwards, C. R. W.; Klenerman, David; Korchev, Yuri E.

doi:10.1016/j.mce.2003.10.015

Cited by 80 publications

(72 citation statements)

References 24 publications

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“…These topographical changes with only a proportion of cells with apical membrane expansion are most likely the result of cell contraction. Previously, we have demonstrated that some A6 epithelial cells show slow lateral contraction under basal conditions (11). Fig.…”

Section: Resultsmentioning

confidence: 72%

“…We have used SICM to image living A6 cells growing on filter supports in physiological conditions (11) to study the mechanism of aldosterone action. Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Because one of the effects of hypotonic treatment is swelling of the cell and, bearing in mind the mechanical sensitivity of the sodium channel (10), we decided to study the morphological changes occurring during aldosterone stimulation and how they related to sodium transport. For this purpose, we have used scanning ion conductance microscopy (SICM), a well developed noninvasive live cell imaging technique (11,12) in combination with patch-clamping (13, 14) that enables us to study not only the topographical changes of the cell apical membrane but also to relate them to ion channel activity. …”

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confidence: 99%

“…The experiments used a single A6 cell line (kindly provided by P. DeSmet, Katholieke Universiteit, Leuven, Belgium) carried out between 127-134 passages. Cells were cultured on membrane filters as described (11,15). …”

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confidence: 99%

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Aldosterone acts via an ATP autocrine/paracrine system: The Edelman ATP hypothesis revisited

Gorelik

Zhang

Sánchez

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Aldosterone, the most important sodium-retaining hormone, was first characterized >50 years ago. However, despite numerous studies including the classical work of Isidore S. ''Izzy'' Edelman showing that aldosterone action depended on ATP production, the mechanism by which it activates sodium reabsorption via the epithelial sodium channel remains unclear. Here, we report experiments that suggest that one of the key steps in aldosterone action is via an autocrine͞paracrine system. The hormone stimulates ATP release from the basolateral side of the target kidney cell. Prevention of ATP accumulation or its removal blocks aldosterone action. ATP then acts via a purinergic mechanism to produce contraction of small groups of adjacent epithelial cells. Patch clamping demonstrates that it is these contracted cells that have channel activity. With progressive recruitment of contracting cells, there is then a parallel increase in transepithelial electrical conductance. In common with other stimuli of sodium transport, this pathway involves phosphatidylinositol 3-kinase. Inhibition of phosphatidylinositol 3-kinase blocks both cell contraction and conductance. We put forward the hypothesis that redistribution of the cell volume caused by the lateral contraction results in apical swelling and that this change, in turn, disrupts the epithelial sodium channel interaction with the F-actin cytoskeleton, opening the channel and hence increasing sodium transport.scanning ion conductance microscopy ͉ scanning probe microscopy ͉ epithelial sodium channel ͉ renal epithelium T he sodium homeostasis regulatory hormone aldosterone was first identified in 1953 (1). Early studies on the biochemical mechanism of its action were reviewed by Edelman and Fimognari (2). This work was greatly helped by the introduction by Ussing and Zerahn (3) of the short-circuit current method for measuring sodium transport. Jean Crabbé (4, 5) first demonstrated the effect of aldosterone on sodium transport across toad bladder and skin and drew attention to the latent period of 60-90 min in the action of aldosterone. He postulated the synthesis or activation of an intermediate by aldosterone. Work by Edelman et al. (6) showed that inhibition of DNA-dependent RNA synthesis and of protein synthesis blocked the aldosterone effect. Edelman et al. (6) went on to demonstrate that aldosterone activation of sodium transport was critically dependent on ATP production. In substrate-depleted toad bladders, aldosterone had little or no effect, but the response was restored by adding glucose or pyruvate to the medium. Edelman suggested that a possible explanation of this absolute dependence on substrate was that, in the energy-depleted system, the rate of activation of sodium transport was limited by the local concentration of ATP. However, he felt that this hypothesis was questionable given that substrate-depleted toad bladders responded to vasopressin and to amphotericin B with a normal increase in sodium transport. This work became known as the ATP generation hypothe...

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Section: Resultsmentioning

confidence: 72%

“…We have used SICM to image living A6 cells growing on filter supports in physiological conditions (11) to study the mechanism of aldosterone action. Fig.…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Aldosterone acts via an ATP autocrine/paracrine system: The Edelman ATP hypothesis revisited

Gorelik

Zhang

Sánchez

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Существенный прогресс в развитии и приме-нении сканирующей ион-проводящей микро-скопии произошел за последние десять-пятнад-цать лет в связи с разработкой модуляционных методик [3][4][5][6] и особенно прыжкового режима ("хоппинг-моды", в английской терминологии -hopping mode) благодаря работам Юрия Корчева и соавторов [7,8]. Именно при применении "хоп-пинг-моды" удалось в полной мере реализовать преимущества сканирующей ион-проводящей микроскопии.…”

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Scanning capillary microscopy

Ahmetova¹,

Yaminsky²

2016

NanoRus

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Сканирующая ион-проводящая микроскопия постепенно эволюционирует в сканирую-щую капиллярную микроскопию, в которой зонд-капилляр играет много различных функ-ций -биосенсора, электрохимического электрода, тест-системы ионов металлов, средства доставки биомакромолекул. Scanning ion conductance microscopy has been evolving into scanning capillary microscopy in which the probe-capillary plays a lot of different functions as biosensor, electrochemical electrode, test system for metal ions, the means for delivery of biomacromolecules.

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