2000
DOI: 10.1002/1099-1387(200009)6:9<459::aid-psc286>3.0.co;2-b
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The use of Seldi ProteinChip? Arrays to monitor production of Alzheimer's ?-amyloid in transfected cells

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Cited by 69 publications
(38 citation statements)
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“…Media were harvested from confluent N2a ⌬E9͞ Swe cells exposed to 10 M STI571 for 16 h. Antibody 6E10 (Signet Laboratories, Dedham, MA) or mouse anti-IgG antibody (ICN) was ligated to Ciphergen PS1 protein chips per the manufacturer's instructions, using the Ciphergen eight-well Bioprocessor Accessory (Ciphergen, Freemont, CA) (19,20). …”
Section: Seldi (Surface Enhanced Laser Desorption͞ionization) Affinitmentioning
confidence: 99%
“…Media were harvested from confluent N2a ⌬E9͞ Swe cells exposed to 10 M STI571 for 16 h. Antibody 6E10 (Signet Laboratories, Dedham, MA) or mouse anti-IgG antibody (ICN) was ligated to Ciphergen PS1 protein chips per the manufacturer's instructions, using the Ciphergen eight-well Bioprocessor Accessory (Ciphergen, Freemont, CA) (19,20). …”
Section: Seldi (Surface Enhanced Laser Desorption͞ionization) Affinitmentioning
confidence: 99%
“…[42]). SELDI provides an assay to monitor production of amyloid-peptides useful in developing treatments for Alzheimer's disease by measuring, in molecular detail, the efficacy of protease inhibitors and other therapeutic interventions [43,44]. SELDI has also been used to investigate the proteases involved in digestion of amyloid precursor protein [45], the role of cholesterol in -amyloid synthesis [46], the effect of a familial mutation in the amyloid precursor protein [47] and whether patterns of amyloid-peptide deposition in animal models mimic the situation in humans [48].…”
Section: Seldi As a Biochemical Toolmentioning
confidence: 99%
“…Also shown are the cleavage sites of cathepsin-D (D), neprilysin (N), insulin degrading enzyme (I), endothelin-converting enzyme 1 (E), plasmin (P), the caspases caspase-6, caspase-8 and caspase-9 (C) and the matrix-metalloproteases MMP-2, MMP-3 and MMP-9 (M) [71][72][73][74][75][76][77][78]. Other cleavage sites due to undescribed mechanisms are shown as unlabelled arrows [79,80]. apparent molecular weight on SDS-PAGE.…”
Section: Induction Of Synaptotoxicity In Admentioning
confidence: 99%