The Use of Selected Bacteria and Yeasts to Control Vibrio spp. in Live Food

Sahandi, Javad; Sorgeloos, Patrick; Xiao, Hui; Wang, Xianghong; Qi, Zhigang; Zheng, Yanfen; Tang, Xuexi

doi:10.3390/antibiotics8030095

Cited by 15 publications

(14 citation statements)

References 44 publications

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“…Despite the previous observations, antibiotic treatment can reduce bacterial load in live feeds but cannot prevent the live feeds from being recolonized within a short period [27], thus suggesting the need to find other therapeutic alternatives. In addition, well-documented toxic effects of florfenicol on fish and crustacean larvae [69][70][71][72] indicate the urgent need for considering the use of alternative strategies to control pathogenic bacteria in live feeds administered to marine fish aquaculture, including bacteriophages, probiotics, and natural products [42,73,74]. In a recent study, probiotics, organic acids, and essential oils were demonstrated to be effective at controlling the pathogenic activity of vibrios on A. franciscana nauplii [75].…”

Section: Discussionmentioning

confidence: 99%

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Hurtado

Miranda

Rojas

et al. 2020

Animals

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Simple Summary: The culture of the marine fish red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibiotics, mainly florfenicol. In this study, the role of live prey (rotifers and the brine shrimp Artemia franciscana) used to feed fish larvae as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis was investigated. Samples of live feeds were collected during the larval growth period and their bacterial contents were determined. High levels of potentially opportunistic pathogens, such as Vibrio spp., as well as florfenicol-resistant bacteria, were detected. Sixty-five florfenicol-resistant isolates were recovered from these cultures and identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%), which exhibited a high incidence of co-resistance to the antibiotics streptomycin, oxytetracycline, co-trimoxazole, and kanamycin. The majority of them carried the florfenicol-resistance encoding genes floR and fexA. The high prevalence of antibiotic-resistant bacteria and the associated genetic elements in live feed administered to reared fish larvae requires the prompt implementation of efficient management strategies to prevent future therapy failures in fish larval cultures and the spread of antibiotic-resistant bacteria to associated aquatic environments. Abstract:The culture of red cusk eel Genypterus chilensis is currently considered a priority for Chilean aquaculture but low larval survival rates have prompted the need for the continuous use of antibacterials. The main aim of this study was to evaluate the role of live feed as a source of antibacterial-resistant bacteria in a commercial culture of G. chilensis. Samples of rotifer and Artemia cultures used as live feed were collected during the larval growth period and culturable bacterial counts were performed using a spread plate method. Rotifer and Artemia cultures exhibited high levels of resistant bacteria (8.03 × 10 4 to 1.79 × 10 7 CFU/g and 1.47 × 10 6 to 3.50 × 10 8 CFU/g, respectively). Sixty-five florfenicol-resistant isolates were identified as Vibrio (81.5%) and Pseudoalteromonas (15.4%) using 16S rRNA gene sequence analysis. A high incidence of resistance to streptomycin (93.8%), oxytetracycline (89.2%), co-trimoxazole (84.6%), and kanamycin (73.8%) was exhibited by resistant isolates. A high proportion of isolates (76.9%) carried the florfenicol-resistance encoding genes floR and fexA, as well as plasmid DNA (75.0%).Animals 2020, 10, 505 2 of 23 microbiota in reared fish larvae, thus proper monitoring and management strategies for live feed cultures appear to be a priority for preventing future therapy failures in fish larval cultures.

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Section: Discussionmentioning

confidence: 99%

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Hurtado

Miranda

Rojas

et al. 2020

Animals

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“…In our in silico analysis, L. buchneri exhibited parasitic interaction limiting the pathogen surivival. Another shortlisted species, L. sakei , isolated from fish and meat products ( Lim et al., 2014 ), reduce the count of Vibrio species in different live prey such as Artemia franciscana , Brachionus plicatilis , and Tigriopus japonicas ( Sahandi et al., 2019 ). It has been reported that L. sakei showed a stronger killing effect on pathogenic V. parahaemolyticus , making it a promising candidate in controlling vibriosis ( Le and Yang, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…We developed a strain-specific genome-scale metabolic model concentrating on Lactobacilli , Bacilli , and Lactococci genera having closed-genome sequences. Moreover, the species from these genera have demonstrated health advantages ( Sahandi et al., 2019 ). The pathogenic strain V. harveyi employed in this study is associated with infections in shellfish, finfish, corals, and molluscs, leading to substantial economic loss to the farmed species in both brackish water and marine aquaculture ( Darshanee Ruwandeepika et al., 2012 ).…”

Section: Introductionmentioning

confidence: 99%

In Silico Prediction of Novel Probiotic Species Limiting Pathogenic Vibrio Growth Using Constraint-Based Genome Scale Metabolic Modeling

Devika

Jangam

Katneni

et al. 2021

Front. Cell. Infect. Microbiol.

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The prevalence of bacterial diseases and the application of probiotics to prevent them is a common practice in shrimp aquaculture. A wide range of bacterial species/strains is utilized in probiotic formulations, with proven beneficial effects. However, knowledge of their role in inhibiting the growth of a specific pathogen is restricted. In this study, we employed constraint-based genome-scale metabolic modeling approach to screen and identify the beneficial bacteria capable of limiting the growth of V. harveyi, a common pathogen in shrimp culture. Genome-scale models were built for 194 species (including strains from the genera Bacillus, Lactobacillus, and Lactococcus and the pathogenic strain V. harveyi) to explore the metabolic potential of these strains under different nutrient conditions in a consortium. In silico-based phenotypic analysis on 193 paired models predicted six candidate strains with growth enhancement and pathogen suppression. Growth simulations reveal that mannitol and glucoronate environments mediate parasitic interactions in a pairwise community. Furthermore, in a mannitol environment, the shortlisted six strains were purely metabolite consumers without donating metabolites to V. harveyi. The production of acetate by the screened species in a paired community suggests the natural metabolic end product’s role in limiting pathogen survival. Our study employing in silico approach successfully predicted three novel candidate strains for probiotic applications, namely, Bacillus sp 1 (identified as B. licheniformis in this study), Bacillus weihaiensis Alg07, and Lactobacillus lindneri TMW 1.1993. The study is the first to apply genomic-scale metabolic models for aquaculture applications to detect bacterial species limiting Vibrio harveyi growth.

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“…However, the application of probiotics to inhibit the growth of Vibrio spp. might be a good method for preventing the pathogen and may also reduce its chances of antibiotic resistance 26 .…”

Section: Antimicrobial Disc Sensitivity Testmentioning

confidence: 99%

Lactic acid bacteria inhibit the growth of Vibrio parahaemolyticus and the invasion of Caco-2 cells

Lua¹,

Lin²,

Hsieh³

et al. 2019

ScienceAsia

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Vibrio parahaemolyticus is a major cause of foodborne illnesses worldwide resulting from the consumption of raw seafood. Lactic acid bacteria (LAB) influence the health of human intestine by enhancing immune system function and acting as antibacterial agents. In this study, 7 LAB (PM12, PM14, PM63, PM212, PM222, BCRC14735, and BCRC17010) were found to adhere to the intestinal epithelial Caco-2 cells as well as to inhibit the growth of V. parahaemolyticus. The viability of Caco-2 cells infected with V. parahaemolyticus was tested by the lactate dehydrogenase (LDH) assay. Our results indicated that PM12, PM14, PM63, PM212, BCRC14735 and BCRC17010 inhibited V. parahaemolyticus BCRC10806 and BCRC12865. While, PM222 could exhibit the maximum comparative adherence ability to Caco-2 cells. The LDH assay results revealed that 5 LAB (PM12, PM14, PM63, PM212, and PM222) significantly decreased the cytotoxicity induced by the V. parahaemolyticus BCRC10806 and BCRC12865 after 2 h of incubation. In the competition assay, the prevention group demonstrated inhibitory effects against two V. parahaemolyticus. Besides, we noted that these 7 LAB inhibited the invasion of V. parahaemolyticus into the Caco-2 cells.

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The Use of Selected Bacteria and Yeasts to Control Vibrio spp. in Live Food

Cited by 15 publications

References 44 publications

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

Live Feeds Used in the Larval Culture of Red Cusk Eel, Genypterus chilensis, Carry High Levels of Antimicrobial-Resistant Bacteria and Antibiotic-Resistance Genes (ARGs)

In Silico Prediction of Novel Probiotic Species Limiting Pathogenic Vibrio Growth Using Constraint-Based Genome Scale Metabolic Modeling

Lactic acid bacteria inhibit the growth of Vibrio parahaemolyticus and the invasion of Caco-2 cells

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