The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts<sup>*</sup>

Wells, Michael A.; Dittmer, John C.

doi:10.1021/bi00906a015

Cited by 425 publications

(120 citation statements)

References 20 publications

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“…Hydrophilic compounds were removed on Sephadex G-25 [9]. The glycolipids and acidic lipids were separated on a silica gel column by conventions techniques.…”

Section: Methodsmentioning

confidence: 99%

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

1980

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“…Hydrophilic compounds were removed on Sephadex G-25 [9]. The glycolipids and acidic lipids were separated on a silica gel column by conventions techniques.…”

Section: Methodsmentioning

confidence: 99%

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

1980

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“…The residue was dissolved in 5 ml chloroform/methanol/water (120: 60:9, by vol. ) and the non-lipid contaminants were removed by passage through a column of Sephadex G-25 as described by Wells & Dittmer (1963). The eluate (approximately 10 ml) was taken to dryness under nitrogen and the residue was dissolved in a 1 ml toluene containing 0.005 % (w/v) BPIT as an antioxidant.…”

Section: E T H 0 D Smentioning

confidence: 99%

The Lipid Fatty Acids of Proteolytic Clostridia

Elsden¹,

Hilton

Parsley

et al. 1980

Microbiology

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“…The lipid extract was taken to dryness under a stream of nitrogen and purified from non-lipid contaminants by passage through a Sephadex G25 Fine column in chloroform/methanol/water (60/30/ Extracts were again evaporated to dryness under a stream of nitrogen with slight heating and dissolved in a small volume of chloroform/methanol (2/1, v/v). Individual lipid species were further separated by thin-4.5, v/v/v) [12]. Lipid spots were visualized by iodine vapour and by autoradiography on Kodirex X-ray film (Kodak).…”

Section: Preparation Of Lipidsmentioning

confidence: 99%

Membrane Lipid Metabolism in Acholeplasma laidlawii A EF 22

Christiansson

Wieslander

1978

European Journal of Biochemistry

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1. Membrane lipid metabolism in Acholeplasma laidlawii A EF 22 has been studied under different conditions by applying three different techniques for changing membrane viscosity : fatty acid and cholesterol supplementation and temperature changes.2. The molar relationship between the two dominating membrane lipids, monoglucosyldiglyceride and diglucosyldiglyceride, is to a large extent determined by membrane viscosity properties. This is shown by the varying metabolic responses occurring during incorporation of different fatty acids with and without cholesterol and by temperature shift-down experiments. Higher viscosity in membranes stimulates synthesis of monoglucosyldiglyceride at the expense of diglucosyldiglyceride. Synthesis of phospho and phosphoglucolipids is affected as well.3. Temperature shift-down from 37 'C to 17 "C results in an immediate synthesis of monoglucosyldiglyceride accompanied by an increased incorporation of unsaturated fatty acids into this lipid. Synthesis of the other membrane lipid species (containing more unsaturated fatty acids) lags behind temporarily.4. Incorporation from an equimolar mixture of palmitic and oleic acids together with cholesterol yields greater amounts of oleic acid in membrane lipids than incorporation in the absence of cholesterol, indicating that incorporation is viscosity dependent.5. Studies of precursor relationships reveal that all main lipids have an active turnover which differs depending on membrane composition and conditions. Furthermore, this turnover proceeds with different intra-lipid pools.6. Isolated membranes contain no detectable lipolytic enzymes capable of hydrolyzing membrane phospho or glycolipids. It is suggested that lipid turnover is partly mediated by enzymatic interlipid conversions, thus not allowing intermediates to accumulate.

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The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts^*

Cited by 425 publications

References 20 publications

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

The Lipid Fatty Acids of Proteolytic Clostridia

Membrane Lipid Metabolism in Acholeplasma laidlawii A EF 22

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The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts*

Cited by 425 publications

References 20 publications

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

A glycolipid containing hopane isolated from the acidophilic, thermophilic bacillus acidocaldarius, has a cholesterol‐like function in membranes

The Lipid Fatty Acids of Proteolytic Clostridia

Membrane Lipid Metabolism in Acholeplasma laidlawii A EF 22

Contact Info

Product

Resources

About

The Use of Sephadex for the Removal of Nonlipid Contaminants from Lipid Extracts^*