The use of single-nucleotide polymorphism in creating a crossline of meat simmentals

Abstract

The article presents the materials of the study on bull cross of the Bredy meat type simmentals, which include the genotyping of animals for the CAST and CAPN1 genes with the identification of frequencies of genotype and alleles, as well as testing the production of these animals expressed in the evaluation of aged within 8 days of meat for the indicators tenderness, juiciness, organoleptic assessment of taste. The results of the studies did not confirm, with certainty, the influence of the CAST gene polymorphism on the beef tenderness indicators, as well as no associations of this gene with the studied juiciness and meat taste indicators, while the carriers of the homozygous CC genotype CAPN1 exceeded other genotypes of the sample in tenderness, juiciness and taste of beef. And indicators of bull meat with a combination of TT* genes on CAST and CC* on CAPN1 had superiority over the average indicators of the total sample (P <0.001), as well as over the group of animals with the most preferred CC genotype for the CAPN1 gene by 1.22 points or 15.4% (P <0.01) by tenderness, by 1.44 points or 18.2% (P <0.05) by juiciness, by 1.56 points or 19.2% (P <0.001) by taste. Studies have confirmed the effect of the CAPN1 gene CC homozygotes polymorphic state itself, as well as the CAST and CAPN1 genes on beef tenderness in the part where the combination of TT genotypes in the CAST gene and CC in the CAPN1 gene gives a positive effect on beef tenderness, probably associated with activation the activity of μ-calpain in connection with the weakening of the effect of calpastatin as an inhibitor on μ-calpain, as a result of which the enzymatic effect on myofibrillary proteins acquires a different intensity, and possibly a different manifestation. What was the reason for the increase in sensory sensitivity to taste of prepared samples, tenderness and juiciness of meat.

The influence of two- or three-breed crossing of young animals of different sexes and directions of productivity on the intake and use of dietary nutrients

“…При этом организации полноценного кормления необходимо уделять постоянное внимание при всех формах ведения скотоводства. Уровень кормления и сбалансированность рационов по основным питательным веществам во многом способствуют проявлению генетического потенциала продуктивности, определяют тем самым энергию роста, живую массу и размеры животного, его экстерьер и убойные качества [7][8][9][10][11][12][13][14].…”

Research Article Identification of SNPs and changes in protein coding of genes associated with beef quality in Nellore cattle

“…In the present study are found some SNPS with results an amino acids changes for the genes studied. Although theses modification could be associated to beef quality characteristics this results should not consider isolate because of according some studies have showing that occurrence of SNPs in different genes can be associated to same characteristic of meat quality in cattle (Bolormaa et al, 2011;Orrù et al, 2011;Tyulebaev et al, 2019).…”

“…Molecular biology techniques have developed rapidly in recent years; they allow the determination of the frequencies and population genetic parameters of genes that are associated with economically relevant characteristics in cattle. In recent years, studies have shown that performance, fattening, carcass and meat quality traits in many cattle breeds are associated with polymorphisms and with genotypic and allelic frequencies of several genes (LEP, CAST, CAPN1, GHR, FABP4, DGAT1, CSN3, LGB, MYF5, TG, IGF1, IGF1R, LGB, ANK1 and OLR1) (Thaller et al, 2003;Curi et al, 2005;Borges et al, 2014;Ardicli et al, 2017;Ardicli et al, 2019a;Ardicli et al, 2019b;Carvalho et al, 2019;Tyulebaev et al, 2019). These traits related to beef quality are very important since they involve economic issues along the beef production chain (farm to fork).…”

“…For the CAST-5 gene, four genotypes were identified in the studied Nellore population, with a predominance of two alleles (A and C). On the other hand, occurrence of different genotypes of the CAST gene in cattle has been reported are frequent identified three genotypes and two alleles like as reporter by Desgarennes-Alcalá et al (2017) for the CAST gene using the PCR-RFLP technique in crossbreeding cattle in Mexico; and by Tyulebaev et al (2019), in 84 Simmental bulls; and by Schenkel et al (2006) for 628 cattle from various breeds (Angus, Limousin, Charolais, Simmental and Others breed). And this author reporter association of these genotypes and alleles for meat quality traits such as meat tenderness, Longissimus dorsi area, lean yield and increase of fat yield.…”

The Impact of Steer Genotype on Protein and Mineral Metabolism

“…The effect of crossbreeding on meat production can only be seen if a full, balanced feed is provided to the crossbreds. This can be monitored by determining the balance of nutrients and minerals in the body of fattened young animals (Kosilov et al, 2006;Nikonova et al, 2014;Kosilov et al, 1997;Bozymov et al, 2016;Speshilova et al, 2014;Nagdalian et al, 2020;Vil'ver et al, 2017;Kosilov et al, 2017;Zadnepryanskii et al, 2012;Kayumov et al, 2019;Mironova et al, 2018;Fatkullin et al, 2018;Sedykh et al, 2018;Tyulebaev et al, 2019).…”