The use of stable isotopes and mass spectrometry in studying lipid metabolism

Beylot, M.

doi:10.1079/pns19940041

Cited by 8 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Independent of these purification procedures, all modern applications utilize isotope dilution for quantification (De Bievre, 1993; Beylot, 1994; Thienpont, Stockl, & de Leenheer, 1995). Stable‐isotope labeled internal standards offer five advantages: As these internal standards are chemically identical and physically similar to the target analytes, maximized correction for errors introduced during sample preparation, storage and detection steps can be achieved. Distinct and parallel detection of unlabeled, endogenous analytes and labeled administered/injected analytes becomes feasible, resulting in minimized uncontrolled, endogenous interferences (e.g., absorption of the studied compound from unknown sources). The fate of different forms of the same compound (free, acetylated, glucuronidated, sulfated) can be specifically monitored provided they are labeled to different degrees. It is possible to study the effect of food structure on the absorption if the compared food types (e.g., solid food or drink) contain the nutrient in differently labeled forms. The fate of nutrients introduced in different modes to the human body (e.g., absorption via lumen or injected into circulation) can be followed distinctly. …”

Section: Technology Reviewmentioning

confidence: 99%

Mass spectrometry in nutrition: Understanding dietary health effects at the molecular level

Kussmann

Affolter

Nagy

et al. 2007

Mass Spectrometry Reviews

View full text Add to dashboard Cite

In modern nutrition research, mass spectrometry has developed into a tool to assess health, sensory as well as quality and safety aspects of food. In this review, we focus on health-related benefits of food components and, accordingly, on biomarkers of exposure (bioavailability) and bioefficacy. Current nutrition research focuses on unraveling the link between dietary patterns, individual foods or food constituents and the physiological effects at cellular, tissue and whole body level after acute and chronic uptake. The bioavailability of bioactive food constituents as well as dose-effect correlations are key information to understand the impact of food on defined health outcomes. Both strongly depend on appropriate analytical tools to identify and quantify minute amounts of individual compounds in highly complex matrices-food or biological fluids-and to monitor molecular changes in the body in a highly specific and sensitive manner. Based on these requirements, mass spectrometry has become the analytical method of choice with broad applications throughout all areas of nutrition research. The current review focuses on selected areas of application: protein and peptide as well as nutrient and metabolite analysis. #

show abstract

Section: Technology Reviewmentioning

confidence: 99%

Mass spectrometry in nutrition: Understanding dietary health effects at the molecular level

Kussmann

Affolter

Nagy

et al. 2007

Mass Spectrometry Reviews

View full text Add to dashboard Cite

show abstract

“…This seems to be a reasonable point at which we might now turn to the more contemporary scene of isotopes in metabolic and nutrition research, a topic that has been the subject of extensive reviews (Halliday & Rennie, 1982;Janghorbani & Young, 1982;Matthews & Bier, 1983;Janghorbani, 1984;Bier, 1987;Wolfe, 1992;Abrams, 1994;Crews et al 1994;Aggett, 1997;Demmelmair et aE. 1997;Patterson, 1997), including presentations at past Nutrition Society meetings (Garlick & Millward, 1972;Halliday & Read, 1981;Buckley, 1988;Beylot, 1994). The focus, for various reasons, will largely be concerned with stable isotopes as tracers, using, where appropriate, some examples taken from the research carried out in our laboratories at the Massachusetts Institute of Technology.…”

Section: Isotopes In Today's Nutrition Researchmentioning

confidence: 99%

“…1997;Patterson, 1997), including presentations at past Nutrition Society meetings (Garlick & Millward, 1972;Halliday & Read, 1981;Buckley, 1988;Beylot, 1994). The focus, for various reasons, will largely be concerned with stable isotopes as tracers, using, where appropriate, some examples taken from the research carried out in our laboratories at the Massachusetts Institute of Technology.…”

Section: Isotopes In Today's Nutrition Researchmentioning

confidence: 99%

Isotopes in nutrition research

Young

Ajami²

1999

Proc. Nutr. Soc.

View full text Add to dashboard Cite

The present lecture begins with a brief overview of the professional and scientific journey taken by Rudolf Schoenheimer, before turning to a discussion of the power of isotopic tracers in nutrition research. Schoenheimer's remarkable contributions to the study of intermediary metabolism and the turnover of body constituents, based initially on compounds tagged with 2H and later with 15N, spanned a mere decade. It is difficult, however, to overestimate the enormous impact of Schoenheimer's research on the evolution of biological science. After a relative hiatus, following Schoenheimer's death in 1941, in the use of stable nuclides as tracers in metabolism and nutrition, especially in human subjects, there is now an expanded and exciting range of techniques, experimental protocols and stable-isotope tracer compounds that are helping to probe the dynamic aspects of the metabolism of the major energy-yielding substrates, amino acids and other N-containing compounds, vitamins and mineral elements in human subjects. Various aspects of the contemporary applications of these tracers in nutrition research are covered in the present lecture.

show abstract

Analysis of Lipids: Triacylglycerols, Phospholipids, Fatty Acids, and Others

Byrdwell

2020

Bailey's Industrial Oil and Fat Products

View full text Add to dashboard Cite

Lipids comprise large and disparate classes of molecules having very different overall polarities, but the one characteristic they typically have in common is a nonpolar hydrocarbon chain that is hydrophobic in nature. The characteristics of each class of lipid dictate the most appropriate tool and approach for analysis. Since many PLs are inherently charged, the polar head group makes them ideally amenable to soft ionization by electrospray ionization (ESI) coupled to MS. In contrast, triacylglycerols (TAGs) are large neutral molecules that are not easily directly ionized by electrospray ionization mass spectrometry (ESI‐MS), and formation of adducts is necessary. Atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI) both readily ionize neutral molecules such as TAGs and sterols. This article will outline the characteristics of each class of lipid, and how these affect the choice for the most appropriate tool for their analysis, including special steps and considerations to be taken into account to optimize analysis of each class. Emphasis will be given to neutral lipids (i.e. TAGs), which are the major components of many edible lipid mixtures. The article will also focus on the approaches necessary to derive the desired structural information from lipids. Many of the principles fundamental to lipid analysis in foods and edible oils were developed for analysis of biological samples, but are directly applicable to fats and oils. This article is intended to serve as a guide for decision‐making, by discussing the strengths and weaknesses and the benefits and shortcomings of the available options for detailed lipid analysis.

show abstract

The use of stable isotopes and mass spectrometry in studying lipid metabolism

Cited by 8 publications

References 42 publications

Mass spectrometry in nutrition: Understanding dietary health effects at the molecular level

Mass spectrometry in nutrition: Understanding dietary health effects at the molecular level

Isotopes in nutrition research

Analysis of Lipids: Triacylglycerols, Phospholipids, Fatty Acids, and Others

Contact Info

Product

Resources

About