The use of stable isotopes to investigate the effects of supplemental lysine and methionine on protein turnover and amino acid utilization in pacu, Piaractus mesopotamicus, juveniles

Abimorad, Eduardo Gianini; Ducatti, Carlos; Castellani, Daniela; Jomori, Rosângela Kiyoko; Portella, Maria Célia; Cameiro, Dalton Jose

doi:10.1016/j.aquaculture.2014.06.006

Cited by 24 publications

(24 citation statements)

References 27 publications

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“…Among 20 AAs serving as building blocks of protein, ten of them were considered as nutritionally essential AAs for fish species (Li, Mai, Trushenski, & Wu, ). Lysine (Lys), in general, the first limiting amino acid in cereals, and its imbalance and bioavailability could affect fish growth and feed efficiency (Abimorad et al, ). Leucine (Leu), a member of branched‐chain AAs (BCAAs) family, had an important anabolic function, stimulating protein synthesis in fish through the target of rapamycin (TOR) signalling pathway (Lansard et al, ).…”

Section: Introductionmentioning

confidence: 99%

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

Wei

Liang

2019

Aquacult Nutr

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The study aimed to investigate the effects of crystalline Lys and Leu, Lys‐Leu dipeptide and Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide, on growth, postprandial Lys and Leu concentrations, gene expression of peptide and amino acid (AA) transporters and related gene expression of protein synthesis pathway in turbot. Three diets (CAA, Di and Tri) contained Lys and Leu as free (Lys and Leu), dipeptide (Lys‐Leu) and tripeptide (Lys‐Leu‐Lys/Leu‐Lys‐Leu) forms, respectively. Improved growth was observed in the Di group compared with the CAA group. For peptide and AAs transporters, PepT1, B0AT1 and y+LAT2 mRNA levels were affected in proximal intestine by dietary treatments. Free Lys and Leu concentrations in the CAA group were significantly higher than that of the Di and Tri groups at 6 hr postfeeding in serum and at 2, 6 and 24 hr postfeeding in muscle. For protein synthesis pathway in muscle, Akt2, TOR and S6k1 gene expression were the highest in the Di group and the lowest in the CAA group, whereas MuRF1 relative expression was the highest in the Tri group. In conclusion, dietary Lys‐Leu dipeptide was utilized more efficiently than free Lys and Leu or Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide for turbot by regulating AAs transport, postprandial AAs concentration and the synthesis of muscle proteins.

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Section: Introductionmentioning

confidence: 99%

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

Wei

Liang

2019

Aquacult Nutr

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“…Results from a preliminary trial aiming to define the conditions for implementing this method in ruminants showed that for a given animal the fraction of 15 N-urea bypassing directly to the urines, without being first incorporated into microbial proteins, was highly variable across time and individuals and led to problems for fitting the progressive urinary 15 N-enrichment post-diet switch (data not shown). Thus, we decided to evaluate the animal 15 N turnover rates during a depletion (Abimorad et al, 2014) rather than enrichment (Bahar et al, 2014) phase. For this, animals were progressively enriched in 15 N over 35 days while adapting to their respective diet; each animal received daily in the morning (0830) a capsule (10 × 3mm) containing 20 mg of 15 N labelled urea (98% APE; Sigma–Aldrich, St. Louis, USA).…”

Section: Methodsmentioning

confidence: 99%

“…A new isotopic approach was originally developed in animal ecophysiology (Fry and Arnold, 1982; Tieszen et al, 1983) to model the rate at which animal’s tissues incorporate the isotopic signature of a new diet in view of accurate food web assessment. It is currently being applied mainly in marine animal species (Abimorad et al, 2014; Mohan et al, 2016) to evaluate the metabolic turnover rate of animal’s tissues. This isotopic method consists in following the dynamic of isotopic change (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, the aim of this study was to adapt the tissue isotopic turnover approach (MacAvoy et al, 2005; Abimorad et al, 2014) to the ruminant’s urine and test it as a non-invasive, less disturbing proxy of the long term WBPT in a relatively large number of growing-fattening beef cattle. In addition, we also measured the isotopic turnover of plasma proteins as an indicator of the fractional protein synthesis rate ( FSR ) of plasma proteins.…”

Section: Introductionmentioning

confidence: 99%

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The isotopic N turnover rate as a proxy to evaluate in the long-term the protein turnover in growing ruminants

Cantalapiedra-Hijar

Fouillet

Chantelauze

et al. 2019

Preprint

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4Protein turnover is an energy-consuming process essential for ensuring the maintenance of 1 5 living organisms. Gold standard methods for protein turnover measurement have inherent 1 6 drawbacks precluding their generalization for large farm animals and during long periods. We 1 7 proposed here a non-invasive proxy of the whole-body fractional protein degradation rate 1 8 (WBPT; whole-body protein turnover for a growing animal) in the long term and in a large 1 9 number of beef cattle. The proxy is based on the rate at which urine-N and plasma proteins 2 0 3 1 non-invasive proxy of the whole-body protein turnover rate in large farm animals. 3 2 3 3 Keywords: protein turnover, isotope, 15 N, ruminant, beef cattle 3 4 3 5

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“…However, the use of radioisotopes has been gradually replaced by methods using stable isotopes tracers [4][5][6][7][8][9][10] where deuterium ( 2 H) or 13 C labelled essential amino acids are used as tracers in combination with Mass Spectrometric detection. Nowadays, the use of stable isotopes is the most widely used procedure for measuring protein synthesis rates.…”

Section: Introductionmentioning

confidence: 99%

A simplified calculation procedure for mass isotopomer distribution analysis (MIDA) based on multiple linear regression

2016

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We have developed a novel, rapid and easy calculation procedure for Mass Isotopomer Distribution Analysis based on multiple linear regression which allows the simultaneous calculation of the precursor pool enrichment and the fraction of newly synthesized labelled proteins (fractional synthesis) using linear algebra. To test this approach, we used the peptide RGGGLK as a model tryptic peptide containing three subunits of glycine. We selected glycine labelled in two C atoms ( C -glycine) as labelled amino acid to demonstrate that spectral overlap is not a problem in the proposed methodology. The developed methodology was tested first in vitro by changing the precursor pool enrichment from 10 to 40% of C -glycine. Secondly, a simulated in vivo synthesis of proteins was designed by combining the natural abundance RGGGLK peptide and 10 or 20% C -glycine at 1 : 1, 1 : 3 and 3 : 1 ratios. Precursor pool enrichments and fractional synthesis values were calculated with satisfactory precision and accuracy using a simple spreadsheet. This novel approach can provide a relatively rapid and easy means to measure protein turnover based on stable isotope tracers. Copyright © 2016 John Wiley & Sons, Ltd.

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The use of stable isotopes to investigate the effects of supplemental lysine and methionine on protein turnover and amino acid utilization in pacu, Piaractus mesopotamicus, juveniles

Cited by 24 publications

References 27 publications

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

The isotopic N turnover rate as a proxy to evaluate in the long-term the protein turnover in growing ruminants

A simplified calculation procedure for mass isotopomer distribution analysis (MIDA) based on multiple linear regression

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