The Use of Staphylococcal Protein A in Diagnostic Virology

Richman, Douglas D.

doi:10.1007/978-3-642-68949-9_10

Cited by 12 publications

(1 citation statement)

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“…Detection can be performed by four broad categories. tion (RPHA); d) single radial hemolysis (SRH); e) thin-layer immunoassay VIA); f) immune diffusion (ID); g) immunoelectroosmophoresis (IEOP), also known as counterimmunoelectrophoresis (CIE); h) gas chromatography (GC); (techniques a-h are described in WHO, 1981); i) latex agglutination (LA); j) passive hemagglutination (PHA); k) heterophil agglutination; 1) hemagglutination inhibition (HI); m) complement fixation (CF) (Giunta and Groppa, 1983); n) neutralization; (techniques i-n are described by Dembert and Kaiser, 1983;Cajal et al, 1983;Russell et al, 1976); 0) monoclonal antibodies (Yolken, 1983); p) immune complex detection (Casali and Oldstone, 1983); q) use of Staphylococcal protein A (Richman, 1983).…”

Section: Introductionmentioning

confidence: 99%

Detection and identification of viruses by electron microscopy

Miller

1986

J. Elec. Microsc. Tech.

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Electron microscopy can aid in the rapid diagnosis of viral diseases, as it can be performed in a matter of hours, but on a routine basis it should be used in conjunction with other techniques. Initially, the specimen source and patient symptoms should be ascertained, as these will lend suggestions of possible agents while eliminating others; however, this information should not be allowed to prejudice observation in such a way as to cause oversight of a n unlikely pathogen. Second, selection of the method of preparation should be based on sample consistency; extraction, debris clarification, concentration, tissue culture amplification, or embedment may be necessary. Finally, false-positive results must be avoided by differentiating viruses from cell organelles or debris, mycoplasmal or bacterial contamination, and bacteriophages.

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