The use of stored skin grafts for keratinocyte cultures

Koller, Rupert; Bierochs, Bettina; Meissl, G.; Bayer, G. S.; Frey, Manfred

doi:10.1054/bjps.2000.3484

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…Although the long‐term cryopreservation of cultured keratinocytes for later application was reported previously,14 but the manipulation processes of cell cryopreservation and refreshing are often complex and would result some cell loss. It has been described that the skin had been stored in 4°C for a short period and then refreshed for the cultivation of keratinocytes 15. The capacity of the oral keratinocytes cultured from the cryopreserved mucosa tissues to fabricate a palatal mucosa equivalent has not been reported previously.…”

Section: Introductionmentioning

confidence: 99%

Cryopreserved lip mucosa tissue derived keratinocytes can fabricate tissue engineered palatal mucosa equivalent

Xiong

Jia

et al. 2010

J Biomed Mater Res

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Clinical application of tissue engineered palatal mucosa is hampered by unavailability of suitable oral keratinocytes as seeding cells. The aim of this study is to fabricate a tissue engineered palatal mucosa equivalent from the oral keratinocytes which cultured from cryopreserved lip mucosa tissues. Abundant lip mucosa tissues during cheilorrhaphy were firstly cryopreserved in liquid nitrogen for four to six months, and then recovered to culture oral keratinocytes for the fabrication of oral mucosa equivalent. In the control groups, oral keratinocytes cultured from fresh lip mucosa, fresh palate mucosa, and cryopreserved palate mucosa were used to fabricate oral mucosa equivalents. Attachment rate of the oral keratinocytes derived from cryopreserved lip mucosa was lower than that of the keratinocytes from fresh lip mucosa samples, however, the cell cycle distribution of oral keratinocytes cultured from all four groups of mucosa samples were similar. Histologically, the fabricated mucosa equivalents from these four groups had four- to six epithelial layers, the basal cells were cubic and the outmost cells were flatten with narrow nuclei which paralleled to the surface of the dermal matrix. Additionally, Ki-67 positive stained cells were mainly located in the basal layer of the epithelium of these equivalents. These characteristics disclosed that the oral mucosa equivalent cultured from the cryopreserved lip mucosa tissue was not different with the equivalents from other groups and similar to the native palate mucosa tissue. It suggested that the cryopreserved lip mucosa tissues could be used for the construction of palatal mucosal equivalent for clinical application.

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Section: Introductionmentioning

confidence: 99%

Cryopreserved lip mucosa tissue derived keratinocytes can fabricate tissue engineered palatal mucosa equivalent

Xiong

Jia

et al. 2010

J Biomed Mater Res

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“…Donor skin samples are mostly acquired from other burn patients and organ donors. In addition, special methods of skin preservation enable the use of skin that has been taken in redundancy for splitthickness skin grafting from non-burned patients and is not needed by them any more [20]. All donors are in general tested for HIV, hepatitis C, hepatitis A, hepatitis B and cytomegalovirus.…”

Section: Indications and Resultsmentioning

confidence: 99%

The Use of Allogeneic Cultivated Keratinocytes for the Early Coverage of Burns - The Viennese Concept

Kamolz¹,

Andel²,

Eisenbock³

et al. 2007

Osteo trauma care

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In the past ten years more than 350 patients suffering from burns were treated in our centre with allogeneic keratinocytes, which were cultivated and transplanted as allogeneic sheet grafts for the coverage of deep dermal defects. In our institution donor skin samples were mostly acquired from other burn patients or organ donors. Seventy-five patients with deep partial thickness burns of the face were treated according to following concept: -Early dermabrasio or tangential excision was performed.-If viable dermis was present, the wounds were covered with allogeneic cultivated keratinocyte sheet grafts. In cases of full-thickness burns, autologous skin grafts were applied. Other indications include deep dermal burns (especially scalds) in children and coverage of early excised deep dermal wounds in adults. In both patient groups, a significant reduction of donorsite morbidity and perioperative blood loss was noted. Moreover, allogeneic keratinocyte sheet grafts were used to cover donor sites of patients with burns exceeding 50 % TBSA. In these patients the use of keratinocytes allowed earlier repeated harvesting of skin grafts. A modified sandwich technique (widely meshed autografts in combination with allogeneic keratinocytes) was used in selected patients who were suffering from very large full-thickness burns ( > 60 % TBSA full thickness). This technique was used in order to decrease epithelialisation time of these widely expanded autologous skin grafts. In our hands the use of allogeneic keratinocyte sheet grafts is a procedure that renders constantly reliable results in deep dermal burns. It minimises the areas of autologous skin harvesting and reduces the amount of blood transfusions.

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“…Nach Abtragen der immunkompeteten Alloepidermis kann die im Wundgrund verbliebene Allodermis dann mit den kultivierten epidermalen Transplantaten gedeckt werden und stellt somit die dringend ben ö tigte dermale Anheftungsstruktur zur Verf ü gung. Bislang ver ö ff entliche Studien belegen, dass durch Anwendung dieser Technik deutlich verbesserte Einheilungsraten und qualitative verbesserte biomechanische Ergebnisse erzielt werden k ö nnen [32,33] .…”

Section: Entwicklung Der Keratinozytenkulturenunclassified

Tissue Engineering von Haut - von der Spalthaut zum gezüchteten Hauttransplantat?

Beier¹,

Boos²,

Kamolz³

et al. 2010

Handchir Mikrochir plast Chir

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Today split or full skin grafts are still the gold standard in the treatment of substance defects of the skin. Such results can be seen, for example, in the therapy for burn patients. However, in patients with more than 50% burned skin area, donor sites are limited. Likewise in chronic wound patients inferior take rates of skin grafts as compared to burn wounds are observed. This may be attributed, for example, to accompanying or underlying chronic diseases or a higher rate of local infections. These phenomena also lead to a lack of availability of transplantable skin grafts. Hence the need for cost effective and user friendly synthetic or engineered skin grafts, which can serve for acute and chronic wounds and which can be also used in critically ill patients, is at hand. During the last 30 years a huge number of biological and synthetic skin graft materials and products based on the patient's own cells were launched on the market. Researchers and clinicians are constantly working on further improvements. One possibility is the engineering of skin grafts in vitro, which have to be integrated into the wound bed after transplantation. Another approach is the fabrication of biocompatible and bioresorbable matrices, which can attract host cells and stimulate a wound-healing process without scars. However, the skin graft materials available today cannot yet replace split or full skin grafts completely because of their inherent limitations such as insufficient take rates and/or the lack of mechanical stability and differentiated structures of the grafted artificial skin. Thus researchers in the field of skin tissue engineering are still working on the final goal of developing a skin graft which has all the features of healthy human skin and is capable of replacing human skin completely. This article gives on overview of the currently available solutions and products in the field of skin tissue engineering.

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The use of stored skin grafts for keratinocyte cultures

Cited by 6 publications

References 6 publications

Cryopreserved lip mucosa tissue derived keratinocytes can fabricate tissue engineered palatal mucosa equivalent

Cryopreserved lip mucosa tissue derived keratinocytes can fabricate tissue engineered palatal mucosa equivalent

The Use of Allogeneic Cultivated Keratinocytes for the Early Coverage of Burns - The Viennese Concept

Tissue Engineering von Haut - von der Spalthaut zum gezüchteten Hauttransplantat?

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