The Use of Supercontinuum Laser Sources in Biomedical Diffuse Optics: Unlocking the Power of Multispectral Imaging

Lange, Frédéric; Giannoni, Luca; Tachtsidis, Ilias

doi:10.3390/app11104616

Cited by 5 publications

(2 citation statements)

References 148 publications

(171 reference statements)

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“…Supercontinuum laser sources can also provide a "white" coherent light source with fast switching, enabling both CW and TD hyperspectral/bNIRS techniques, albeit with added cost and complexity. 52 Examples of supercontinuum-based hyperspectral systems have recently been described elsewhere. 53,54 The detection arm of a hyperspectral system is often a single camera (either CCD or CMOSbased technology).…”

Section: Hyperspectral and Broadband Nirsmentioning

confidence: 99%

Optical imaging and spectroscopy for the study of the human brain: status report

et al. 2022

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. This report is the second part of a comprehensive two-part series aimed at reviewing an extensive and diverse toolkit of novel methods to explore brain health and function. While the first report focused on neurophotonic tools mostly applicable to animal studies, here, we highlight optical spectroscopy and imaging methods relevant to noninvasive human brain studies. We outline current state-of-the-art technologies and software advances, explore the most recent impact of these technologies on neuroscience and clinical applications, identify the areas where innovation is needed, and provide an outlook for the future directions.

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Section: Hyperspectral and Broadband Nirsmentioning

confidence: 99%

Optical imaging and spectroscopy for the study of the human brain: status report

et al. 2022

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“…In the last 20 years, supercontinuum laser sources covering wide spectral bandwidths at high spectral power and fast switching have been developed. Lange et al [2] contributed a timely and comprehensive review of the features and biomedical and clinical applications of supercontinuum laser sources.…”

Section: Review Of Issue Contentsmentioning

confidence: 99%

Advances in Hyperspectral and Multispectral Optical Spectroscopy and Imaging of Tissue

Toronov

2022

Applied Sciences

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Transportable hyperspectral imaging setup based on fast, high-density spectral scanning for in situ quantitative biochemical mapping of fresh tissue biopsies

Giannoni,

Marradi,

Scibilia

et al. 2024

J. Biomed. Opt.

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.SignificanceHistopathological examination of surgical biopsies, such as in glioma and glioblastoma resection, is hindered in current clinical practice by the long time required for the laboratory analysis and pathological screening, typically taking several days or even weeks to be completed.AimWe propose here a transportable, high-density, spectral scanning-based hyperspectral imaging (HSI) setup, named HyperProbe1, that can provide in situ, fast biochemical analysis, and mapping of fresh surgical tissue samples, right after excision, and without the need for fixing, staining nor compromising the integrity of the tissue properties.ApproachHyperProbe1 is based on spectral scanning via supercontinuum laser illumination filtered with acousto-optic tunable filters. Such methodology allows the user to select any number and type of wavelength bands in the visible and near-infrared range between 510 and 900 nm (up to a maximum of 79) and to reconstruct 3D hypercubes composed of high-resolution (4 to 5 μm), widefield images (0.9×0.9 mm2) of the surgical samples, where each pixel is associated with a complete spectrum.ResultsThe HyperProbe1 setup is here presented and characterized. The system is applied to 11 fresh surgical biopsies of glioma from routine patients, including different grades of tumor classification. Quantitative analysis of the composition of the tissue is performed via fast spectral unmixing to reconstruct the mapping of major biomarkers, such as oxy-(HbO2) and deoxyhemoglobin (HHb), as well as cytochrome-c-oxidase (CCO). We also provided a preliminary attempt to infer tumor classification based on differences in composition in the samples, suggesting the possibility of using lipid content and differential CCO concentrations to distinguish between lower and higher-grade gliomas.ConclusionsA proof of concept of the performances of HyperProbe1 for quantitative, biochemical mapping of surgical biopsies is demonstrated, paving the way for improving current post-surgical, histopathological practice via non-destructive, in situ streamlined screening of fresh tissue samples in a matter of minutes after excision.

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The Use of Supercontinuum Laser Sources in Biomedical Diffuse Optics: Unlocking the Power of Multispectral Imaging

Cited by 5 publications

References 148 publications

Optical imaging and spectroscopy for the study of the human brain: status report

Optical imaging and spectroscopy for the study of the human brain: status report

Advances in Hyperspectral and Multispectral Optical Spectroscopy and Imaging of Tissue

Transportable hyperspectral imaging setup based on fast, high-density spectral scanning for in situ quantitative biochemical mapping of fresh tissue biopsies

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