The Use of the Isotope <sup>51</sup>Cr as a Label for Red Cells

Mollison, P. L.; Veall, N.

doi:10.1111/j.1365-2141.1955.tb05489.x

Cited by 325 publications

(82 citation statements)

References 11 publications

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“…T h e rat blood was kept overnight at 2°C in this mixture, with addition of formaldehyde to a final concentration of 0.5%. It was then centrifuged at 600 g for 10 rain and the sedimented cells were resuspended in Strumia mixture and incubated for 5 m i n at 37°C with 10 #curies of Na-51CrO4 (Rachromate 51, Abbott Laboratories, Chicago, Ill.) (26). The ceils were washed free from excess chromate by five to six washings with Strumia mixture and injected intravenously in an a m o u n t corresponding to 0.2-0.3 ml of blood per 100 g rat.…”

Section: Methodsmentioning

confidence: 99%

Lysosomes in Lymphoid Tissue

Bowers

Duve

1967

The Journal of Cell Biology

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The density-distribution patterns of various enzymes and of labeled materials have been determined by isopycnic centrifugation in a sucrose-0.2 M KC1 gradient on homogenates of lymphoid tissues from rats injected with Triton WR-1339, 14C-labeled dextran, 51Cr-labeled erythrocytes, and cortisol. The results confirm and extend the conclusion, derived from previous investigations on normal animals, that the lysosomes of lymphoid tissues form two and possibly three, distinct populations. The evidence indicates that the L19 population belongs to macrophages and the Lx5 group to lymphocytes. The L30 population appears to be associated with a special type of phagocyte with a high capacity for dextran storage. All three populations seem to contribute to the activities found in soluble form in homogenates of normal lymphoid tissues.

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Section: Methodsmentioning

confidence: 99%

Lysosomes in Lymphoid Tissue

Bowers

Duve

1967

The Journal of Cell Biology

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“…Since the number of red cells in the marrow suspension was 100-fold greater than the number of nucleated marrow cells the "1Cr was principally in the red cells (48) while the '9Fe was principally in erythroblasts (49,50 (48,51,52) and is attached nonspecifically to many proteins. The overriding predominance of the red cell concentration allows the use of this isotope for the labeling of red cells in clinical studies (48). While the patient's pretreatment YG-globulins produced a large increase in the release of '9Fe from the cells into the medium, there was no increase in the release of 51Cr.…”

Section: Measurement Of Cytotoxicitymentioning

confidence: 99%

Studies on Red Cell Aplasia. V. PRESENCE OF ERYTHROBLAST CYTOTOXICITY IN γG-GLOBULIN FRACTION OF PLASMA

Krantz¹,

Moore²,

Zaentz³

1973

J. Clin. Invest.

128

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A B S T R A C T The marrow cells of a patient with pure red cell aplasia markedly increased their rate of heme synthesis when they were freed from the host environment and were incubated in vitro. When the red cell aplasia was treated with cyclophosphamide and prednisone, marrow cell incorporation of 'Fe into heme in vitro increased several weeks before a reticulocytosis was apparent, and was the earliest effect noted. The plasma 'YG-globulins of this patient inhibited heme synthesis by normal marrow cells or the patient's own marrow cells obtained after remission of the disease.Since the inhibition of heme synthesis could be the result of damage to erythroblasts, the patient's posttreatment marrow cells or normal marrow cells were labeled with '9Fe and were then incubated with the patient's pretreatment, treatment, and posttreatment ,YG-globulins as well as normal YG-globulins. At the end of this incubation the supernatant and cells were separated and counted. Heme was extracted and also was counted. Treatment of the cells with the patient's pretreatment YG-globulins resulted in a release of 40% of the radioactive heme from the cells. This represented the loss of radioactive hemoglobin and was an index of erythroblast cytotoxicity. A progressive disappearance of the cytotoxic factor in the YG-globulins occurred in the 3 wk period preceding the onset of reticulocytes in the patient's blood. Posttreatment and normal yG-globulins did not produce this effect and increased injury of red cells and lymphocytes was not produced by the patient's pretreatment yG-globulins. These studies demonstrate a method for measuring erythroblast cytoxicity and show that red cell aplasia is associated with YG-globulins that This study was presented in part at

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“…After a few minutes of insertion of the appropriate blood samples, the values are. Sources of errors in this case are the different hematocrit readings obtained on samples of blood drawn from different parts of the cardiovascular system, and leakage of albumin from the cardiovascular fluid compartment [29][30][31]. B) Radio-active chromium: Second in line is this method in which a sample of patient's blood is labeled with Cr 51 , as described above, and flowed back to the cardiovascular system.…”

Section: A) Radioactive Iodine (I)mentioning

confidence: 99%

“…After a few minutes, a sample of venous blood is withdrawn and the patient's total red cell volume is calculated pre and post surgically, from the hematocrit reading. This method is précised for measuring blood volume loss, but again it is elaborative and requires expensive equipment [28,30,31].…”

Section: A) Radioactive Iodine (I)mentioning

confidence: 99%

Intra-Operative Hemorrhage: A Review of Literature

Kathariya

2013

J Med Diagn Meth

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Despite the tremendous innovation in field of medical science and technology; intraoperative hemorrhage remains one of the major surgical complications encountered in day-to-day surgical practice. The morbidity and mortality associated with surgical hemorrhage are considerable and it remains a restraining factor for advanced surgical procedures. Intra operative hemorrhage may sometime cause significant complications and may even lead to alarming events.Clinical Significance: Clinicians should know the amount of blood loss during the surgical procedures to detect and treat any aberrant changes immediately. There are very few published reports on different techniques to measure intra-operative hemorrhage. However, there are on reports, which compile all the different techniques, which could be used for measuring intra-operative bleeding. This review compiles various ways to measure intra-operative blood loss to update and alert clinicians about its consequences.

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The Use of the Isotope ⁵¹Cr as a Label for Red Cells

Cited by 325 publications

References 11 publications

Lysosomes in Lymphoid Tissue

Lysosomes in Lymphoid Tissue

Studies on Red Cell Aplasia. V. PRESENCE OF ERYTHROBLAST CYTOTOXICITY IN γG-GLOBULIN FRACTION OF PLASMA

Intra-Operative Hemorrhage: A Review of Literature

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The Use of the Isotope 51Cr as a Label for Red Cells

Cited by 325 publications

References 11 publications

Lysosomes in Lymphoid Tissue

Lysosomes in Lymphoid Tissue

Studies on Red Cell Aplasia. V. PRESENCE OF ERYTHROBLAST CYTOTOXICITY IN γG-GLOBULIN FRACTION OF PLASMA

Intra-Operative Hemorrhage: A Review of Literature

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The Use of the Isotope ⁵¹Cr as a Label for Red Cells