The use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L)

Hauton, Chris; Hawkins, L. E.; Hutchinson, S.A.

doi:10.1016/s0305-0491(98)00036-4

Cited by 68 publications

(47 citation statements)

References 18 publications

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“…There is also a measurable decrease in bonamiasis prevalence in the 2 areas in the UK (Fal and South Coast harbours) most likely affected by naturally increasing seawater temperatures. However, Hauton et al (1998) found that immuno-competence of oysters was best at intermediate (15°C) temperature, provided salinity was high, and Engelsma et al (2010) found evidence suggesting that mortality in flat oysters increased at higher water temperatures. So although prevalence of the disease may be lower at higher temperatures, the oysters may be more likely to die.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of Bonamia in the UK, 1982 to 2012

Laing

Dunn²,

Peeler³

et al. 2014

Dis. Aquat. Org.

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it was found in 2 new areas, one in Wales and one in Scotland. Subsequent further spread to additional areas in all parts of the UK has resulted in 9 zones being currently designated as infected with the disease. In addition, a single oyster from one area has tested positive for the closely related B. exitiosa. In general, analysis of the results of the monitoring programme in England and Wales shows no clear trend in infection levels over time, although there has been an apparent decrease in the level of infection in some fishery areas. In an autumn sampling programme the highest levels of infection were detected in October.

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Section: Discussionmentioning

confidence: 99%

Epidemiology of Bonamia in the UK, 1982 to 2012

Laing

Dunn²,

Peeler³

et al. 2014

Dis. Aquat. Org.

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“…In addition, the NRR assay has recently been shown to be a useful indicator of bivalve cellular stress associated with natural environmental perturbation, such as the e#ects of salinity and temperature on Ostrea edulis L. (Hauton et al, 1998) and tidal emersion on Mya arenaria (L.) and Mytilus edulis (L.) (Tremblay & PellerinMassicotte, 1997).…”

Section: Introductionmentioning

confidence: 99%

Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg) and the scallop Pecten maximus (L.)

Hauton

Hawkins

Hutchinson

2001

Fish & Shellfish Immunology

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“…It was demonstrated that lysosome stability and membrane integrity of hemocytes have a close relationship with animal health status (Lowe et al, 1995;Yao et al, 2008). Furthermore, stress from low temperature also can reduce NRR time; this effect was observed in cold-stressed M. galloprovincialis (Hauton et al, 1998;Camus et al, 2000). A recent study of thermal acclimation in the mussel M. barbatus found decreases in NRR retention time during prolonged acclimation at high temperatures (28 and 30°C) (Dimitriadis et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Reductions in hemocyte viability and NRR time appear to be generally useful indices of cellular stress. Previous studies have also demonstrated that hemocyte viability and lysosome membrane stability decreased when the animal suffered from environmental stress or pathogenic infection (Hauton et al, 1998;Camus et al, 2000;Yao et al, 2008;Parolini et al, 2011).…”

Section: Hemocyte Viability and Lysosome Integritymentioning

confidence: 99%

“…Damage to lysosomes is one example of this type of stress-induced lesion. Lysosomes are found within the semi-granular and granular hemocytes of many marine invertebrates and are released by a process of degranulation of hemocytes after environmental stresses (Hauton et al, 1998;Camus et al, 2000). Once in the cytoplasm, the proteolytic enzymes that exist in lysosomes are released and the hemocytes are lysed (Yao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

Yao

Somero

2012

Journal of Experimental Biology

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SUMMARYWe investigated the effects of acute heat stress and cold stress on cell viability, lysosome membrane stability, double-and singlestranded DNA breakage, and signaling mechanisms involved in cellular homeostasis and apoptosis in hemocytes of native and invasive mussels, Mytilus californianus and Mytilus galloprovincialis, respectively. Both heat stress (28, 32°C) and cold stress (2, 6°C) led to significant double-and single-stranded breaks in DNA. The type and extent of DNA damage were temperature and time dependent, as was caspase-3 activation, an indicator of apoptosis, which may occur in response to DNA damage. Hemocyte viability and lysosomal membrane stability decreased significantly under heat stress. Western blot analyses of hemocyte extracts with antibodies for proteins associated with cell signaling and stress responses [including members of the phospho-specific mitogen-activated protein kinase (MAPK) family c-JUN NH 2 -terminal kinase (JNK) and p38-MAPK, and apoptosis executor caspase-3] revealed that heat and cold stress induced a time-dependent activation of JNK, p38-MAPK and caspase-3 and that these signaling and stress responses differed between species. The thermal limits for activation of cell signaling processes linked to the repair of stress-induced damage may help determine cellular thermal tolerance limits. Our results show similarities in responses to cold and heat stress and suggest causal linkages between levels of DNA damage at both extremes of temperature and downstream regulatory responses, including induction of apoptosis. Compared with M. californianus, M. galloprovincialis might have a wider temperature tolerance due to a lower amount of single-and double-stranded DNA damage, faster signaling activation and transduction, and stronger repair ability against temperature stress.

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The use of the neutral red retention assay to examine the effects of temperature and salinity on haemocytes of the European flat oyster Ostrea edulis (L)

Cited by 68 publications

References 18 publications

Epidemiology of Bonamia in the UK, 1982 to 2012

Epidemiology of Bonamia in the UK, 1982 to 2012

Response of haemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg) and the scallop Pecten maximus (L.)

The impact of acute temperature stress on hemocytes of invasive and native mussels (Mytilus galloprovincialisandM. californianus): DNA damage, membrane integrity, apoptosis and signalling pathways

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