The use of the zebrafish as a model in fish aquaculture research

Piferrer, Francesc; Ribas, Laia

doi:10.1016/bs.fp.2020.10.003

Cited by 9 publications

(10 citation statements)

References 227 publications

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“…Fish were held in 9 liters (L) tanks on a recirculating system (Aquaneering, San Diego, CA) in a chamber with a photoperiod of 12 h of light and 12 h of darkness, an air temperature of 26 ± 1°C and a humidity of 60 ± 3%. Physicochemical parameters were monitored daily, staying at appropriate conditions [ 26 , 27 ]: water was maintained at (28 ± 0.2°C), pH (7.2 ± 0.5), conductivity (750–900 μS) and dissolved oxygen (6.5–7.0 mg/l) with a water pump of 3,000 L/h and a UV light system to eliminate any possible bacteria in the water. Sulfite, sulfate, nitrate, and ammonia quality parameters were checked weekly using commercial kits.…”

Section: Methodsmentioning

confidence: 99%

“…Gravity alteration experiments have been conducted using a wide range of animal models, including Drosophila [ 17 ], Caenorhabditis elegans [ 18 ], mice and rats [ 19 , 20 ], and fish [ 21 ]. Among these, zebrafish ( Danio rerio ), a tropical freshwater fish, belonging to the teleost family, has become an unprecedented tool for research in a variety of fields, such as genetics [ 22 ], development [ 23 ], toxicology [ 24 ], physiology [ 25 ], aquaculture [ 26 , 27 ], among others. Its short generation time, the large amount of fertilized eggs, its transparency during early development, the high similarity with human orthologous genes (~70%), and, the availability of genomic resources, make zebrafish an excellent animal research model.…”

Section: Introductionmentioning

confidence: 99%

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Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2024

PLoS ONE

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Gravity is one of the most constant environmental factors across Earth’s evolution and all organisms are adapted to it. Consequently, spatial exploration has captured the interest in studying the biological changes that physiological alterations are caused by gravity. In the last two decades, epigenetics has explained how environmental cues can alter gene functions in organisms. Although many studies addressed gravity, the underlying biological and molecular mechanisms that occur in altered gravity for those epigenetics-related mechanisms, are mostly inexistent. The present study addressed the effects of hypergravity on development, behavior, gene expression, and most importantly, on the epigenetic changes in a worldwide animal model, the zebrafish (Danio rerio). To perform hypergravity experiments, a custom-centrifuge simulating the large diameter centrifuge (100 rpm ~ 3 g) was designed and zebrafish embryos were exposed during 5 days post fertilization (dpf). Results showed a significant decrease in survival at 2 dpf but no significance in the hatching rate. Physiological and morphological alterations including fish position, movement frequency, and swimming behavior showed significant changes due to hypergravity. Epigenetic studies showed significant hypermethylation of the genome of the zebrafish larvae subjected to 5 days of hypergravity. Downregulation of the gene expression of three epigenetic-related genes (dnmt1, dnmt3, and tet1), although not significant, was further observed. Taken altogether, gravity alterations affected biological responses including epigenetics in fish, providing a valuable roadmap of the putative hazards of living beyond Earth.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2024

PLoS ONE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fish were held in 9 liters (L) tanks on a recirculating system (Aquaneering, San Diego, CA) in a chamber with a photoperiod of 12 h of light and 12 h of darkness, an air temperature of 26 ± 1 ◦C and a humidity of 60 ± 3%. Physicochemical parameters were monitored daily, staying at appropriate conditions (Ribas & Piferrer, 2014; Piferrer & Ribas, 2020): water was maintained at (28 ± 0.2°C), pH (7.2 ± 0.5), conductivity (750– 900 µS) and dissolved oxygen (6.5–7.0 mg/l) with a water pump of 3,000 L/h and a UV light system to eliminate any possible bacteria in the water. Sulfite, sulfate, nitrate and ammonia quality parameters were checked weekly using commercial kits.…”

Section: Methodsmentioning

confidence: 99%

“…Gravity alteration experiments have been conducted using a wide range of animal models, including Drosophila (Bae et al, 2016), Caenorhabditis elegans (Honda et al, 2014), mice and rats (Ross, 1994; Jamon, 2014), and fish (Aceto et al, 2016). Among these, zebrafish ( Danio rerio ), a tropical freshwater fish, belonging to the teleost family, has become an unprecedented tool for research in a variety of fields, such as genetics (Norton & Bally-Cuif, 2010), development (Yalcin et al 2017), toxicology (Dai et al 2014), physiology (Briggs, 2002), aquaculture (Ribas & Piferrer, 2014; Piferrer & Ribas, 2020), among others. Its short generation time, the large amount of fertilized eggs, its transparency during early development, the high similarity with human orthologous genes (∼70%) and, the availability of genomic resources, make zebrafish an excellent animal research model.…”

Section: Introductionmentioning

confidence: 99%

Epigenetic and physiological alterations in zebrafish subjected to hypergravity

Salazar,

Joly,

Anglada-Escudé

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Gravity is one of the most constant environmental factors across Earth’s evolution and all organisms are adapted to it. Consequently, spatial exploration has captured the interest in studying the biological changes that physiological alterations are caused by gravity. In the last two decades, epigenetics has explained how the environmental cues are able to altered gene functions in the organisms. Although many studies addressed gravity, the underlying biological and molecular mechanisms that are occurred in altered gravity for those epigenetics-related mechanisms, are mostly inexistent. The present study addressed the effects of hypergravity on development, behavior, gene expression, and most importantly, on the epigenetic changes in a world-wide animal model, the zebrafish (Danio rerio). To perform hypergravity experiments, a custom-centrifuge simulating the large diameter centrifuge (100 rpm ∼ 3g) were designed and zebrafish embryos were exposed during 5 days post fertilization (dpf). Results showed a significant decrease of survival at 2 dpf but not significance in the hatching rate. Physiological and morphological alterations including fish position, movement frequency and swimming behavior showed significant changes due to hypergravity. Epigenetic studies showed a significant hypermethylation of the genome of the zebrafish larvae subjected to 5 days of hypergravity. A downregulation of the gene expression of three epigenetic-related genes (dnmt1,dnmt3, andtet1), although not significant, were further observed. Taken altogether, gravity alterations affected biological responses including epigenetics in fish, providing a valuable roadmap of the putative hazards of living beyond Earth.

show abstract

“…Epigenetic mechanisms, namely, DNA methylation, histone modifications and non-coding RNAs, regulate gene expression by integrating genomic and environmental information, thus contributing to phenotypic plasticity (Duncan et al, 2014). Epigenetics holds great promise in aquaculture (Gavery and Roberts, 2017;Granada et al, 2018;Piferrer et al, 2015). Epigenetic alterations in response to environmental stimuli, for example, changes in DNA methylation levels in specific loci in the genome that correlate to a certain phenotype, can be used as epigenetic biomarkers.…”

Section: Introductionmentioning

confidence: 99%