The Use of Thiol/Disulfide Homeostasis Parameters in the Diagnosis of Acute Appendicitis in Children

Abstract

Objective The aim of this study was to determine the role of thiol/disulfide homeostasis (TDH) parameters which are new oxidative stress markers for the diagnosis of acute appendicitis (AA) in children with abdominal pain complaints. Methods A total of 40 patients with AA and 45 patients with abdominal pain were included in this study. Two groups were compared in terms of age, gender, white blood cell count, neutrophil-to-lymphocyte ratio, C-reactive protein (CRP), appendix diameter, and TDH parameters (native and total thiol levels, native thiol/total thiol ratio [antioxidant parameters] and disulfide level, disulfide/native thiol ratio, and disulfide/total thiol ratio [oxidant parameters]). Results Compared with the abdominal pain group, it was found that disulfide level, disulfide/native thiol, and disulfide/total thiol ratios (p < 0.001 for all) in patients with AA were significantly higher and their native thiol level (p = 0.02) and native thiol/total thiol ratio (p < 0.001) were significantly lower. There was no significant difference between the two groups in terms of total thiol level. There was no significant difference between the perforated and nonperforated groups in patients with AA in terms of TDH parameters. In addition, the CRP level was higher in the perforated group than the nonperforated group. Conclusion To our knowledge, the present study is a first in the literature in which TDH parameters were used in children with AA compared with the abdominal pain group. We thought that these parameters may be useful as new biomarkers supporting AA diagnosis in children.

Evaluation of Systemic Immune-Inflammation Index as Novel Marker in the Diagnosis of Acute Appendicitis in Children

“…Patients with appendicitis are likely to have an elevated CRP measurement, and many studies have found that CRP is useful in diagnosing appendicitis. [1][2][3]6] Narci et al reported in their study that the CRP value was higher in the AA group [2] Duman et al stated that CRP values increased significantly in perforated appendicitis. [3] In our study, the CRP value was found to be significantly higher in the perforated appendicitis group compared to the nonperforated group.…”

“…The most preferred laboratory tests are WBC value, NP, CRP, NLR, PLR, MPV, and serum sodium level. [2][3][4][5][6][14][15][16][17] In a meta-analysis study conducted by Acharya et al for the diagnosis of AA, the place of values such as WBC, CRP, bilirubin, procalcitonin, Ddimer and interleukin-6 in the diagnosis was investigated. Sensitivity, specificity, and AUC for WBC were 79%, 55%, and 0.75%, and 76%, 50%, and 0.80% for CRP, respectively.…”

“…[18] Similar to the studies previously reported in the literature, neutrophil, lymphocyte, WBC values, NLR and PLR rates are compared to the control group, the AA group was found to be significantly higher in the AA group. [6,[14][15][16][17][18] Pehlivanlı et al reported in their study that PLR value could be a new biomarker for the diagnosis of appendicitis, and it could be of and predictive value in differentiation of acute appendicitis from perforated appendicitis also. [16] In our study, the PLR value was significantly higher in the AA group than in the control group.…”

“…[1,2,5] Recently it has been reported in many publications that measurements made from blood values are beneficial in the diagnosis of AA; Neutrophil-lymphocyte ratio (NLR), serum sodium level, mean platelet volume (MPV), platelet-lymphocyte ratio (PLR) are the most commonly used ones. [1][2][3][4][5][6] The systemic immune inflammation index (SII), which contains peripheral neutrophils, lymphocytes, and platelets, has recently been defined in some studies. [7][8][9] It has been reported in some studies that elevated as an inflammation marker.…”

The role of thiol-disulfide and ischemia-modified albumin in the differential diagnosis of acute scrotum in children

“…The measurement of plasma or serum thiol-disulfide balance via the colorimetric method was developed by a Turkish scientist. [21] There are many studies on OS markers and their relationship with various diseases, such as acute appendicitis, in children, [22][23][24] yet no studies exist on the relationship between acute scrotum and OS markers in children. Additionally, many studies have explored the relationship between thiol-disulfide and testis torsion; however, our study is important as the first to show thioldisulfide and IMA levels in the differential diagnosis of acute scrotum in children.…”