The Use of Touch DNA Analysis in Forensic Identification Focusing on Short Tandem Repeat-Combined DNA Index System Loci THO1, CSF1PO and TPOX

Yudianto, Ahmad; Nuraini, M Indah; Furqoni, Abdul Hadi; Nzilibili, Simon Martin Manyanza; Harjanto, Pudji

doi:10.4081/idr.2020.8716

Cited by 6 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies continue to elucidate the ability to generate DNA profiles from samples taken from touched objects [61][62][63][64]. Additional studies evaluated means of generating human DNA profiles from traces of biological material left on various items, includ-ing hair [65], bricks [66], dogs [67], bite marks in food [68], paper documents [69], eye wear [70], superabsorbent polymer-containing products (commonly found in nappies and sanitary towels) [71], improvised explosive devices [72], rifle magazines [73] and bullet cartridges [74][75][76][77][78].…”

Section: Samplingmentioning

confidence: 99%

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Oorschot

Meakin

Kokshoorn

et al. 2021

Genes

View full text Add to dashboard Cite

Understanding the factors that may impact the transfer, persistence, prevalence and recovery of DNA (DNA-TPPR), and the availability of data to assign probabilities to DNA quantities and profile types being obtained given particular scenarios and circumstances, is paramount when performing, and giving guidance on, evaluations of DNA findings given activity level propositions (activity level evaluations). In late 2018 and early 2019, three major reviews were published on aspects of DNA-TPPR, with each advocating the need for further research and other actions to support the conduct of DNA-related activity level evaluations. Here, we look at how challenges are being met, primarily by providing a synopsis of DNA-TPPR-related articles published since the conduct of these reviews and briefly exploring some of the actions taken by industry stakeholders towards addressing identified gaps. Much has been carried out in recent years, and efforts continue, to meet the challenges to continually improve the capacity of forensic experts to provide the guidance sought by the judiciary with respect to the transfer of DNA.

show abstract

Section: Samplingmentioning

confidence: 99%

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Oorschot

Meakin

Kokshoorn

et al. 2021

Genes

View full text Add to dashboard Cite

show abstract

“…The Federal Bureau of Investigation (FBI) and the world's forensic expert community designed 13 locus as a national forensic identification system that synergizes with the Combined DNA Index System (CODIS) database. For personal identification, the researcher chose the TPOX and TH01 locus because several studies have shown that the TPOX and TH01 locus have strong discriminating power in Asian populations 9 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of DNA Quality, TPOX Locus, TH01 Locus in Seawater Soaked Nails in Forensic Identification

Utomo,

Endarini,

Yudianto

et al. 2023

JIK

View full text Add to dashboard Cite

Forensic science is multiplying. One of them is the identification of events at sea. Of course, the victim’s body can be submerged in seawater for days so that components in seawater can affect the victim’s body. This research aimed to see the effect of storage time on the level and purity of DNA in nail samples. Identification of nail DNA using the Polymerase Chain Reaction (PCR) method. The DNA purity test examines nail samples using a spectrophotometer read at 260 and 280 nm wavelengths. Measurement of DNA levels using the PCR instrument on the STR TPOX locus, the result was 32.02 ug/uL on positive control, 36.73 ug/uL in seawater immersion on the 2nd day, 35.35 ug/uL in seawater immersion on the 7th day, and 34.5 ug/uL in seawater immersion on the 20th day. At the STR TH01 locus, the result was 32.155 ug/uL on positive control, 38.05 ug/uL on the 2nd day of immersion in seawater, 35.18 ug/uL on the 7th day, and 33.88 ug/uL on the 20th day. Results Examination of the Total Plate Number of seawater on the 2nd day, 7th day, and 20th day of immersion found no colonies of germ colony growth. This study obtained sufficient DNA purity from the 2nd day, 7th day and 20th day. So that the samples could be analyzed for DNA levels, the result obtained from the study showed a decrease in DNA levels at the TPOX Locus and TH01 Locus from the 2nd day, 7th day, and 20th day.

show abstract

“…The succession of natural catastrophic events (tsunamis and hurricanes), of terrorist attacks (11 September 2001 in New York, 11 March 2003 in Madrid and London, 13 November 2015 in Paris), air accidents and events related to the increasing frequency of use of industrial and military artifices, have determined the most urgent need to activate particular procedures aimed at solving problems of a medico-legal and pathological forensic nature [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. One of the most commonly accepted definitions is that a mass disaster is a situation that involves criticality between the number of victims and resources, in terms of both men and means, present on the site of the event which is mostly unexpected and sudden [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Forensic Analysis and Identification Processes in Mass Disasters: Explosion of Gun Powder in the Fireworks Factory

et al. 2021

View full text Add to dashboard Cite

A mass disaster is a situation that involves criticality between the number of victims and resources, in terms of both men and means, present on the site of an event that is mostly unexpected and sudden. In the multidisciplinary teams that intervene, the role of forensic pathologists, who are responsible for the direction and coordination of post-mortem operations, is central, and must remain so. The authors report the case of an explosion of a pyrotechnic artifice factory, as a result of which numerous victims and injuries are recorded. So, the team completed the autopsies and created a protocol to obtain biological samples (bones, blood, teeth, muscles), while the forensic pathologists contacted the families of the alleged victims and each provided a blood sample that was collected for the DNA. The geneticist, using the method of gene extraction and amplification, obtained the DNA from each bone, tooth, and muscle of blood taken from the victims and then compared it with that extracted from the blood samples of the relatives; the electropherograms showed at least one allele for each genetic marker of the “Combined DNA Index System” in common between the victims and the families, thus allowing to establish the identity of all the subjects involved in the event. Having established the identity of all workers, it was possible to determine their whereabouts in the environment at the time of the location of fires and explosions. The results of the various forensic analyzes (autopsies, genetic investigations and even traumatological investigations) have allowed us to validate a scientific method useful in all mass disasters even when any type of anthropological or forensic dental research is difficult.

show abstract

The Use of Touch DNA Analysis in Forensic Identification Focusing on Short Tandem Repeat-Combined DNA Index System Loci THO1, CSF1PO and TPOX

Cited by 6 publications

References 22 publications

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

DNA Transfer in Forensic Science: Recent Progress towards Meeting Challenges

Characterization of DNA Quality, TPOX Locus, TH01 Locus in Seawater Soaked Nails in Forensic Identification

Forensic Analysis and Identification Processes in Mass Disasters: Explosion of Gun Powder in the Fireworks Factory

Contact Info

Product

Resources

About