The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX

Abstract

Introduction:Identification through DNA analysis is an accurate diagnostic tool. DNA analysis is via Variable Number of Tandem Repeat (VNTR) and Restriction Fragment Length Polymorphisms (RFLP). Up to date, blood spots/blood, sperm spots, vaginal swabs, buccal swabs and bones are specimens that are widely used in the field of forensics for DNA analysis. At the crime scene, the perpetrator's skin may accidentally be in contact with surrounding objects thereby transferring trace evidence to the objects. Method and Materials:Laboratory observation to demonstrate identification through DNA isolation from the objects that are touched (touch DNA), using the STR CODIS locus, with a momentary research design. DNA was isolated from buccal swabs and swab properties (watches and mobile phones) from volunteers who have signed the consent form. A total of ten samples were used in this study.