The use of transrectal massage combined with artificial vagina as semen collection in javan banteng (Bos javanicus) bull

Abstract

Semen collection in wild animals may be challenging for some methods such as electro ejaculator may cause pain and distress to the animals. Transrectal massage has been widely used in domestic and wild animals semen collection. Even though an artificial vagina may provide a condition that mimics the female reproductive tract and known to provide better results in some species, the application may face obstacles in the field. This study was conducted to evaluate the possibility of modified semen collection in banteng bull by combining the transrectal massage method followed by the use of an artificial vagina. The bull was habituated and trained for semen collection in chute under veterinarian supervision in Taman Safari Indonesia, Cisarua, Bogor, West Java. Semen samples from one Javan banteng bull were collected once a week. Banteng bull showed stud and penile protrusion during semen collection. Watery semen with volume ranged from 12 ml were successfully collected, with sperm progressive motility ranged from 25%-40%. Our study also showed sperm abnormalities comprising 18.11% abnormal sperm heads, 28.82% abnormal sperm tails and 0.94% teratoid form. The results demonstrated the possibility of this modified method as an alternative procedure for semen collection in banteng bull which may also be useful for other wild animals in field conditions

Correlation between Post‐Thaw Spermatozoa Quality of the Endangered Javan Banteng with OPN Gene Expression

“…Terefore, our study's results were still below those of previous studies. Although not all the variables evaluated in the study showed signifcant diferences between the two banteng bulls, the value of progressive motility and viability of the Javan banteng spermatozoa in this study were still below the results of previous studies by Johnston et al [16] and Yoelinda et al [36].…”

Successful semen collection and cryopreservation of Banteng semen (Bos javanicus) using an artificial vagina

“…The results of the six batches showed a variation in the observed parameters. The The semen volume produced in this study is higher than the previously described by the electroejaculation method [13] and transrectal massage [17]. In the previous study [13,17], semen volume ranged from 4 to 7.4 mL (6.3±0.76) for electroejaculation and between 1 to 2 mL for transrectal massage combined with an artificial vagina.…”

“…The The semen volume produced in this study is higher than the previously described by the electroejaculation method [13] and transrectal massage [17]. In the previous study [13,17], semen volume ranged from 4 to 7.4 mL (6.3±0.76) for electroejaculation and between 1 to 2 mL for transrectal massage combined with an artificial vagina. It is well known that visual and olfactory stimuli play an essential role in male sexual behavior [21].…”

“…However, these methods require the animal to be trained and acclimated to the collection procedure and are less suitable for wild animals. In Banteng, the use of an artificial vagina combined with transrectal massage has already been tried, but the results are not satisfactory in terms of quantity [17]. The objective of this study is to develop semen collection further using an artificial vagina and to investigate the sperm motility of Banteng after freezing in a commercial extender.…”