The use of vinylcyclohexene diepoxide to create diminish ovarian reserve model on rats

Abstract

Background: Infertility remains a significant issue in the world of health. Now Assisted Reproductive Technology (ART) is widely used to help couples with infertility. In ART diminish ovarian reserve patients have a very low success rate of pregnancy. To help patients with Diminished Ovarian Reserve (DOR), research is conducted using an animal model. vinylcychlohexene diepoxide (VCD) has ovotoxic effects. This study looked at the use of VCD in inducing targeted DOR conditions in animals (rats), create a model that could be used for future animal studies in infertility. Methods: Adult female Rattus norvegicus were used in this study. All were given VCD injections at 80 mg/kg intraperitoneally for 15 days. An examination of Follicle Stimulating Hormone (FSH) levels will be carried out on days 0,3,5,7, and 15. To mimic IVF process, all rats got an injection of 10 IU of Pregnant Mare Serum Gonadotropin (PMSG) and 10 IU human Chorionic Gonadotropin (hCG) to confirm DOR. Ovarectomy was performed, dominant follicles were taken, denudation was carried out, and the oocytes were cultured for 12 hours. Then, oocytes were assessed through a microscope. Results: Starting from day 5 post VCD induction, there was a significant increase in the FSH level in the group of rats that were induced with the administered VCD dose compared to the control group that experienced normal FSH fluctuations. The FSH concentration reached >50%. It was found that the number of degenerated oocytes had an average of 1.11. Oocytes that were in Germinal-Vesicle (GV) stage had an average of 1.89, while in the Metaphase I (M1) stage the average is 2.882. Lastly, the number of oocytes in the Metaphase (MII) stage which is fully matured had an average of 1.117. Conclusions: VCD can become an important tool for future studies that needs an animal model with DOR.