Background: The use of lights and siren transport (LST) has been a matter of debate because of the short time savings and well-established increased risks for Emergency Medical Services (EMS) and bystanders. Time-critical hospital intervention (TCHI) denotes urgently needed procedures that cannot be performed properly in an out-of-hospital setting. Since 2013, rapid transportation from the field – fast-track – is currently used for patients with acute ST-elevation myocardial infarction, suspicion of acute stroke and out-of-hospital cardiac arrest. We aimed to determine whether the use of LST was associated with the realization of TCHI for non-trauma cases within 15 minutes of hospital arrival, to identify the predictors of TCHI and to compare clinical outcomes in patients transported with or without LST. Methods: This is a monocentric prospective observational study of non-trauma patients transported by ambulance. Based on Ross et al.’s work in 2016 on trauma patients, TCHI procedures were developed by the study team. We used descriptive statistics to determine whether the use of LST was associated with the realization of TCHI. Univariate and multivariate analyses determined the predictors of TCHI and compared clinical outcomes. Results: On the 324 patients included, 67 (20.7%) benefitted from LST, with 40 (59.7%) receiving TCHI ( p < 0.001). The most common medical TCHI was the fast-track (65.2% of all TCHI). LST was predictive of the need for TCHI ( p < 0.001), as was the clinical condition of the patient and also when EMS providers expected TCHI. When transported with LST, patients were hospitalized significantly more often in acute care units and less often in general wards or discharged from the Emergency Department ( p < 0.001). Conclusions: The use of LST for non-trauma patients was positively associated with the realization of TCHI. EMS providers demonstrated a high level of precision in discerning which patients deserved LST. A majority of the LST benefitted from TCHI. Nevertheless, when fast-track was excluded the rate dropped by more than 50%. To reduce the rate of over-triage (LST without TCHI), LST should be used only for fast-track and also when TCHI is expected by the EMS providers.