The usefulness of lactate dehydrogenase measurements in current oncological practice

Forkasiewicz, Agata; Dorociak, Maja; Stach, Kamilla; Szelachowski, Piotr; Taboła, Renata; Augoff, Katarzyna

doi:10.1186/s11658-020-00228-7

Cited by 151 publications

(112 citation statements)

References 86 publications

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“…It is known that LDH in the blood of cancer patients is usually elevated, which gives reason to consider LDH as a sufficiently informative indicator to monitor the treatment dynamics of patients with some cancer types [7][8]. In this work, it was found that LDH level in both blood plasma and tumor tissue (per 1 mg of protein) in mice treated with oxamate at both doses did not differ significantly from the corresponding index in the control.…”

Section: Fig 2 the Effect Of Oxamate On The Growth And Metastasis Omentioning

confidence: 64%

Evaluation of antitumor effect of oxamate on metastatically active cells

Kolesnik¹,

Pyaskovskaya²,

Solyanik³

2020

The European Potential for Development of Natural Science

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Section: Fig 2 the Effect Of Oxamate On The Growth And Metastasis Omentioning

confidence: 64%

Evaluation of antitumor effect of oxamate on metastatically active cells

Kolesnik¹,

Pyaskovskaya²,

Solyanik³

2020

The European Potential for Development of Natural Science

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“…(B3A1 or H3M1), LDH3 (B2A2 or H2M2), LDH4 (B1A3 or H1M3), and LDH5 (A4 or M4). The buffer system at pH 8.6 was chosen for the best separation of the ve LDH isoenzymes (30)(31)(32)(33). Because the B polypeptide has more acidic amino acid residues than the A polypeptide, LDH1/B has the highest migration rateand LDH5/A has the lowest migration rate.…”

Section: Ldh Zymographymentioning

confidence: 99%

“…LDH isoenzyme pattern of subcutaneously located murine glioblastoma tumors LDH zymography is a common method to detect LDH isoenzymes and provides for the direct observation of 5 isozyme bands in the active state (31). The LDH isoenzyme pattern for GL261, CT2A and ALTS1C1 LDH-A KD and NC subcutaneous tumors were compared to each other and to heart and skeletal muscle tissue from the same animals (Fig.…”

Section: Effects Of Ldh-a Knockdown On In Vitro and In Vivo Growth Prmentioning

confidence: 99%

LDH-A Knockdown: Changes in The LDH Isoenzyme Profile and Variability in Glioma Response

Shindo

Maeda

et al. 2021

Preprint

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Background: Lactate metabolism in tumors is now recognized as a major energy source and a major gluconeogenic precursor for many tumors, as well as shown to exhibit signaling properties. There is less information on the role of the LDH/lactate axis in brain tumors, although lactate formation in gliomas is associated with poor survival. Methods: Three murine glioma cell lines (GL261, CT2A, and ALTS1C1) were transduced to knockdown (KD) expression of the murine LDH-A gene. The effects of the LDH-A KD were compared to those in control (NC) cells and tumors. Results: Differences in the expression of LDH-A and LDH-B mRNA, protein, and enzymatic activity were observed in the six cell lines. LDH zymography showed a major difference in LDH subunit distribution between GL261 LDH-A KD and NC tumors, whereas little or no effect of LDH-A KD was observed in CT2A and ALTS1C1 tumors. Tumors LDH-A and LDH-B immunohistochemistry and a Weka segmentation analysis were consistent with isoenzyme patterns and the above analyses. An “inverse” LDH-A/LDH-B staining relationship (high vs low) was observed in many local GL261 tumor regions. In contrast, CT2A tumors showed a more “direct” local LDH-A/LDH-B staining relationship. LDH-A KD prolonged the doubling time of GL261 cells in culture and prevented the formation of subcutaneous flank tumors in immune-competent C57BL/6 mice (GL261 NC tumors had a prolonged growth delay). In nude mice, both LDH-A KD and NC GL261 tumors grew more rapidly than GL261 NC tumors in C57BL/6 mice. No differences between NC and KD cell proliferation (in vitro) and tumor growth in C57BL/6 mice (doubling time) were observed for CT2A and ALTS1C1 cells and tumors, consistent with the absence of a difference in their LDH isoenzyme profiles. Conclusions: These results show the combined impact of a genetic alteration (LDH-A depletion) on the LDH isoenzyme profile, expression of LDH-A vs LDH-B and LDH enzymatic activity, and the immune system (C57BL/6 vs nude mice) on the growth of s.c. located tumors.

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“…The lactate dehydrogenase (LDH) is a cytosolic enzyme, known as the most common enzymes in nature and acts as a hydrogen-transfer enzyme [1] . It serves as a biomarker of disturbed cell molecular integrity generated by pathological situations [2] .…”

Section: Introductionmentioning

confidence: 99%

The Significance of Elevated Lactate Dehydrogenase and Creatine Kinase Activities as Prognostic Biomarkers for the Mortality in Patients with Terminal Cancers

AL-Daami,

Al-Hindy,

Kaurshead

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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Background: Both creatine kinase (CK) and/or lactate dehydrogenase (LDH) mutually are elevated significantly in many tumors. This study hypothesizes that LDH was evaluated as a prognostic factor for survival time in patients with terminal cancer (PTC). Additionally, CK considered as a valuable in assessing prognosis of advanced cancer patients. This study aimed to estimate the change in the CK/LDH levels in patients with different types of cancer comparing with the controls, and to investigate their prognostic significance for survival time termination in PTC. Methodology:The study was performed at Cancer Central Research Clinic in Baghdad. We quantitated CK/ LDH using the colorimetric method in sixty PTC, and twenty age/sex-matched healthy adults.Results: Serum values of CK/LDH were significantly increased in PTC compared to the controls (p<0.001). There was a weak non-significant correlation between CKLDH in PTC and control. There was a nonsignificant correlation of CK to LDH levels in different cancer types, except in patients with lymphatic cancer. Conclusion:In agreement with our hypothesis, serum levels of CK/LDH were significantly increased as the patients approached death.

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The usefulness of lactate dehydrogenase measurements in current oncological practice

Cited by 151 publications

References 86 publications

Evaluation of antitumor effect of oxamate on metastatically active cells

Evaluation of antitumor effect of oxamate on metastatically active cells

LDH-A Knockdown: Changes in The LDH Isoenzyme Profile and Variability in Glioma Response

The Significance of Elevated Lactate Dehydrogenase and Creatine Kinase Activities as Prognostic Biomarkers for the Mortality in Patients with Terminal Cancers

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