The usefulness of liquid-based cytology for endoscopic ultrasound-guided tissue acquisition of solid pancreatic masses

Itonaga, Masahiro; Ashida, Reiko; Kitano, Masayuki

doi:10.3389/fmed.2022.943792

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…Additionally, LBC enables multiple specimen preparation. Multiple samples can then be used for immunohistochemical and genetic examinations (38). The use of LBC in BCC cytodiagnosis is an interesting research gap.…”

Section: Liquid-based Cytology and Genetic Testingmentioning

confidence: 99%

Basal cell carcinoma cytology revisited – a modern perspective on an old technique

DURIKOVA

2023

BLL

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Basal cell carcinoma is the most frequently occurring cancer in humans. In light of its vast prevalence, this disease causes a substantial burden on the quality of life of patients. Histopathologic examination is currently the gold standard for diagnostic confi rmation and a requisite for therapeutic planning. Cytology possesses several advantages compared to biopsy. This is namely due to its minimal invasiveness, absence of scarring, improved aesthetic outcome, cost-effectiveness, and procedural simplicity. This review focused on contemporary evidence on basal cell carcinoma cytology to provide a comprehensive description of the technique with practical insights for specifi c clinical scenarios. This review also aimed to delineate and discuss evidence gaps and potential novel applications of this technique in the context of recent advances in cytopathology, dermatosurgery, molecular targeted therapy, and precision medicine (Tab. 2, Ref. 43).

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Section: Liquid-based Cytology and Genetic Testingmentioning

confidence: 99%

Basal cell carcinoma cytology revisited – a modern perspective on an old technique

DURIKOVA

2023

BLL

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“…Cytological examinations may also involve the evaluation of liquid‐based cytology (LBC) specimens. 61 In LBC, collected cells are suspended in a liquid medium, homogenized, and smeared onto a glass slide. This method has been found to prevent blood contamination and the drying or depletion of cells, which are known drawbacks of SC.…”

Section: Introductionmentioning

confidence: 99%

“…This method has been found to prevent blood contamination and the drying or depletion of cells, which are known drawbacks of SC. 61 A meta‐analysis comparing LBC and SC with ROSE for SPLs obtained via EUS‐TA found that SC had significantly higher sensitivity than LBC (90% vs. 60%; p < 0.05), suggesting that LBC might be less effective in facilities where ROSE is available. 62 By contrast, work from our group showed that the combination of LBC plus SC had significantly higher diagnostic accuracy than SC alone (94.1% vs. 69.6%; p < 0.001), suggesting that the combination of LBC plus SC is superior to LBC alone or SC alone in the clinical evaluation of SPLs.…”

Section: Introductionmentioning

confidence: 99%

Updated techniques and evidence for endoscopic ultrasound‐guided tissue acquisition from solid pancreatic lesions

Itonaga,

Ashida,

Kitano

2024

DEN Open

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Endoscopic ultrasound‐guided tissue acquisition (EUS‐TA), including fine‐needle aspiration (EUS‐FNA) and fine‐needle biopsy (EUS‐FNB), has revolutionized specimen collection from intra‐abdominal organs, especially the pancreas. Advances in personalized medicine and more precise treatment have increased demands to collect specimens with higher cell counts, while preserving tissue structure, leading to the development of EUS‐FNB needles. EUS‐FNB has generally replaced EUS‐FNA as the procedure of choice for EUS‐TA of pancreatic cancer. Various techniques have been tested for their ability to enhance the diagnostic performance of EUS‐TA, including multiple methods of sampling at the time of puncture, on‐site specimen evaluation, and specimen processing. In addition, advances in next‐generation sequencing have made comprehensive genomic profiling of EUS‐TA samples feasible in routine clinical practice. The present review describes updates in EUS‐TA sampling techniques of pancreatic lesions, as well as methods for their evaluation.

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“…[ 2 ] It is a rapid, sensitive, specific, and cost-effective method with a minimum rate of side effects. [ 3 , 4 ]…”

Section: Introductionmentioning

confidence: 99%

Comparison of FNA-based conventional cytology specimens and digital image analysis in assessment of pancreatic lesions

Khozeymeh,

Ariamanesh,

Roshan

et al. 2023

Cytojournal

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Objectives: Endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) is one of the most important diagnostic tools for investigation of suspected pancreatic masses, although the interpretation of the results is controversial. In recent decades, digital image analysis (DIA) has been considered in pathology. The aim of this study was to assess the DIA in the evaluation of EUS-FNA based cytopathological specimens of pancreatic masses and comparing it with conventional cytology analysis by pathologist. Material and Methods: This study was performed using cytological slides related to EUS-FNA samples of pancreatic lesions. The digital images were prepared and then analyzed by ImageJ software. Factors such as perimeter, circularity, area, minimum, maximum, mean, median of gray value, and integrated chromatin density of cell nucleus were extracted by software ImageJ and sensitivity, specificity, and cutoff point were evaluated in the diagnosis of malignant and benign lesions. Results: In this retrospective study, 115 cytology samples were examined. Each specimen was reviewed by a pathologist and 150 images were prepared from the benign and malignant lesions and then analyzed by ImageJ software and a cut point was established by SPSS 26. The cutoff points for perimeter, integrated density, and the sum of three factors of perimeter, integrated density, and circularity to differentiate between malignant and benign lesions were reported to be 204.56, 131953, and 24643077, respectively. At this cutting point, the accuracy of estimation is based on the factors of perimeter, integrated density, and the sum of the three factors of perimeter, integrated density, and circularity were 92%, 92%, and 94%, respectively. Conclusion: The results of this study showed that digital analysis of images has a high accuracy in diagnosing malignant and benign lesions in the cytology of EUS-FNA in patients with suspected pancreatic malignancy and by obtaining cutoff points by software output factors; digital imaging can be used to differentiate between benign and malignant pancreatic tumors.

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The usefulness of liquid-based cytology for endoscopic ultrasound-guided tissue acquisition of solid pancreatic masses

Cited by 8 publications

References 38 publications

Basal cell carcinoma cytology revisited – a modern perspective on an old technique

Basal cell carcinoma cytology revisited – a modern perspective on an old technique

Updated techniques and evidence for endoscopic ultrasound‐guided tissue acquisition from solid pancreatic lesions

Comparison of FNA-based conventional cytology specimens and digital image analysis in assessment of pancreatic lesions

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