The Ustilago maydis regulatory subunit of a cAMP-dependent protein kinase is required for gall formation in maize.

Signaling via cAMP in fungi: interconnections with mitogen-activated protein kinase pathways

“…The ubc1 gene also appears to be required for pathogenicity. Microscopic observation of infected maize tissue showed that a dikaryon that is defective in both alleles of ubc1 is capable of growth in the plant, but is incapable of tumor formation (Gold et al 1997). Similarly, diploid strains lacking both copies of the ubc1 gene are also incapable of forming tumors on plants (Dürrenberger et al 1998).…”

“…This is suggested by the fact that mutants defective in the cAMP pathway are attenuated for the mating reaction and virulence (Gold et al 1997;Dürrenberger et al 1998). Furthermore, the cAMP and mating pathways apparently share the same Gα-subunit, Gpa3p (Kahmann and Basse 1997;Regenfelder et al 1997).…”

Examination of the relevance of PI3K and PTEN pathway in Ustilago maydis.

“…The strains of U. maydis used in this study are presented in Table 1 (Gold et al, 1997) used in this study were provided by Cau Pham (Univ. of Louisville).…”

“…Burpee & Co., Warminster, PA) was performed as previously described (Gold et al, 1997). Virulence was rated by a disease index on a scale of 0 to 5 with 0= no symptoms, 1 = chlorosis and / or anthocyanin biosynthesis, 2 = small leaf galls, 3 = large leaf galls and / or small stem galls, 4 = large stem galls, and 5 = plant death from infection.…”

“…maydis cells having a concentration of roughly 10 7 cells ml -1 were used for plate mating assay (Mayorga and Gold, 1999) and plant infection (Gold et al, 1997) Liquid YEPS media was inoculated with strains and shaken 24 hours to exponential phase of growth (A600 of 0.5 to 0.8), as measured spectrophotometrically. For charcoal plate matting, 10 µl volume was spotted for each mating-mixture and photos were taken at 24 and 48 hours post plating.…”