The utilities of Citrus tissue culture

Chamandoosti, Firoozeh

doi:10.25125/agriculture-journal-ijoear-sep-2017-10

Cited by 4 publications

(2 citation statements)

References 75 publications

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“…Nesse processo, é possível a obtenção de haploides e duplo haploides em uma única etapa, obtendo-se assim, indivíduos homozigotos, que irão aumentar a eficiência dos programas de melhoramento genético (GERMANÁ, 2020). A produção de calos embriogênicos em citros pode ser produzida pela cultura in vitro de nucelos excisados, óvulos abortivos, óvulos não fertilizados, óvulos não desenvolvidos, anteras, estilos e estigmas, epicótilos, cotilédones e segmentos de raiz (CHAMANDOOSTI, 2017).…”

Section: Introductionunclassified

Avaliação da capacidade de indução de calos embriogênicos em óvulos abortados de citros

Santos

Cardoso

Souza

et al. 2022

CLIUM

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O cultivo de óvulos abortados se constitui uma das formas para obtenção de calos embriogênicos, que, por sua vez, incide em uma via para a obtenção de indivíduos haploides em citros. Assim, o objetivo desse trabalho foi estimular a formação de calos embriogênicos mediante o cultivo in vitro de óvulos abortados de genótipos de citros. Para isso, foram coletados frutos das laranjeiras ‘Azeda’ clone Jacarandá e ‘Hamlin’ clone CNPMF 04, do limoeiro ‘Cravo’ clone Santa Cruz e das tangerineiras ‘Sunki Maravilha’, ‘Sunki Tropical’ e ‘Cleópatra’ para extração dos óvulos abortados, que foram desinfestados em câmara de fluxo laminar e introduzidos no meio de cultura MT suplementado com 50 g L-1 de sacarose e 500 mg L-1 de extrato de malte e posteriormente mantidos no escuro a 27±1ºC. Obteve-se formação de calos embriogênicos em todos os genótipos estudados, em taxas que variaram de 7% a 54,5%, na tangerineira ‘Sunki Tropical’ e na laranjeira ‘Azeda’ clone Jacarandá, respectivamente. Diante disso, ainda são necessários ajustes no meio de cultura, a fim de aumentar os percentuais de calogênese, contudo os calos obtidos já podem ser utilizados para a etapa de regeneração in vitro.

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Section: Introductionunclassified

Avaliação da capacidade de indução de calos embriogênicos em óvulos abortados de citros

Santos

Cardoso

Souza

et al. 2022

CLIUM

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“…Grosser et al (1988) reported that protoplasts can be isolated from friable embryogenic ovule-derived calli maintained on growth-regulator-free media. Chamandoosti (2017) reported that the production of embryogenic callus lines in citrus can be produced by the in vitro culture of excised nucelli, abortive ovules, unfertilized ovules, undeveloped ovules, juice vesicles, anthers, styles and stigmas, epicotyls, cotyledons, and root segments.Thus, for researches specifically focused on variety or rootstock improvements using somatic hybridization in citrus, a reliable callus induction protocol is essential. However, the efficiency of existing protocols may vary depending on the genotype used.…”

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Efficient protoplast isolation from ovule-derived embryogenic callus inCitrusvolkameriana

Çimen¹

2020

Turk J Agric For

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The present study reports on the isolation of viable protoplast from ovule-derived embryogenic calli of Volkameriana (Citrus volkameriana L.), which is a rootstock in high demand for lemon production.Ovules of C. volkameriana isolated at 3 different immature fruit stages, comprising4, 8, and 12 weeks after anthesis (WAA),were cultured on5 different media in order to produce embryogenic callus lines as a source material for protoplast isolation. EME medium (MT basal medium + 0.5 gL–1 malt extract), with the addition of phytohormones [kinetin (KIN), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP)] at different concentrations, were tested for callogenesis. According to 2-way ANOVA, significant effects were determined as a result of the immature fruit stage and type of culture media (P ≤ 0.01) on the callogenesis and embryogenic callus induction frequency. First, callus formation was recorded after 4 WAA on medium comprising EME + 2,4-D (1.0 mg L–1) + BAP (0.5 mg L–1). Callus induction frequency was the highest (90.00%) in the same culture medium when the ovules wereculturedat8 WAA. In addition, culturing the ovules isolated from 12 WAA immature fruits of C. volkameriana resulted in the highest indirect somatic embryogenesis (24%). Embryogenic callus initiation was the highest (25.56%) using EME + KIN (1.0 mg L–1) and ovules cultured at 8 WAA (14%) resulted in the highest embryogenic callus formation. Effects of different enzyme concentrations on the efficiency of protoplast isolation were calculated using the hemocytometer cell counting method. The combination of 2% cellulase and 0.2% pectinase gave the highest numbers of protoplasts, at 12.33 × 105protoplast/mL. Embryogenic callus lines obtained by culturing ovules of C. volkameriana yielded high-quality protoplasts after isolation and could be useful as a protoplast source for further somatic hybridization studies.

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Callogenesis and plant regeneration of sweet orange cv. Washington Navel from floral organ cultures

Metoui¹,

Nahdi²,

Dhaouadi³

et al. 2020

J. Hortic. Sci. Technol.

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Washington Navel orange (Citrus sinensis L.) can be infected with virus and virus like diseases that affect not only the production but also fruit quality and the plant’s longevity. For viral sanitation, Washington Navel regeneration was investigated in vitro via floral organ culture. Flowers were collected before opening from healthy Washington Navel trees kept under greenhouse. Floral organs (style/stigma and ovary) were cultured on Murashige and Skoog (MS) medium containing various plant growth regulators combinations of naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The highest rate of callogenesis (95%) was obtained from style/stigma explant cultures on MS medium enriched with 3 mgL-1 BAP, which also resulted in 100% rooted plantlets. Ovary cultures did not show any success on the culture medium with various plant growth regulators combinations. The acclimatization success of rooted plantlets by grafting on Citrus volkameriana rootstocks was about 83%. Thus, these results can be used for mass production of disease-free citrus plants and improve sanitation program of the local citrus genotypes in Tunisia.

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The utilities of Citrus tissue culture

Abstract: Abstract-Citrus is the third most important fruit crop in the world

Cited by 4 publications

References 75 publications

Avaliação da capacidade de indução de calos embriogênicos em óvulos abortados de citros

Avaliação da capacidade de indução de calos embriogênicos em óvulos abortados de citros

Efficient protoplast isolation from ovule-derived embryogenic callus inCitrusvolkameriana

Callogenesis and plant regeneration of sweet orange cv. Washington Navel from floral organ cultures

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