The Utility of 4-(2-Thienyl)Pyridines as a Derivatization Reagent for HPLC and CE

Abstract

To emphasize the capability of a fluorescent thienylpyridine skeleton, the derivatization of carboxylic acid, aldehyde, ketone, and glycine were examined and applied to the following determinations: glycine with 5-(4-pyridyl)-2-thiophenecarbaldehyde using CE, carboxylic acids with 5-(4-pyridyl)-2-thiophenemethanol or 4-(5-diazomethyl-2-thienyl)pyridine using HPLC, carboxylic acids with 1-(2-ammonioethyl)-4-(2-thienyl)pyridinium dibromide using CE, and aldehydes and ketones with 4-(2-thienyl)pyridinium acetohydrazide chloride.