The Utility of MALDI-TOF-Mass Spectrometry, Analytical Profile Index (API) and Conventional-PCR for the Detection of Foodborne Pathogens from Meat

Ramatla, Tsepo; Ngoma, Lubanza; Mwanza, Mulunda

doi:10.12691/jfnr-9-8-7

Cited by 5 publications

(3 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Te bacterial universal primers (27F: AGA GTT TGA TCM TGG CTC AG and 1492R: GGT TAC CTT GTT ACG ACT T) targeting the 16S rRNA gene segment were used for molecular identifcation using PCR. Te PCR conditions were as follows: initial denaturation step at 96 °C for 4 minutes, followed by 30 cycles of denaturation at 94 °C for 30 seconds, annealing at 57 °C for 30 seconds, and extension at 72 °C for 1 minute, and fnally, a single and fnal extension step at 72 °C for 10 minutes [22].…”

Section: Identifcation Of Salmonella Species Using 16s Rrnamentioning

confidence: 99%

Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa

Mlangeni,

Ramatla,

Lekota

et al. 2024

International Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

Reptiles are carriers of an array of microorganisms, including significant zoonotic bacteria of the genus Salmonella, which cause a disease referred to as salmonellosis that affects both animals and humans. This study investigated the occurrence of Salmonella serovars in wild reptiles at Timbavati Private Game Reserve in Limpopo Province, South Africa, and examined their virulence and antimicrobial resistance gene profiles. A total of 19 wild reptiles were sampled, which resulted in 30 presumptive Salmonella isolates. The isolates were identified using polymerase chain reaction (PCR) by amplifying the invA gene and were further confirmed by 16S rRNA gene sequencing. Salmonella serovars were detected in chameleons (36.8%), lizards (31.6%), snakes (15.8%), and tortoises (15.8%). The use of 16S rRNA gene sequencing revealed that Salmonella enterica subsp. enterica serovar Salamae (30%), S. enterica subsp. enterica (16.7%), S. enterica subsp. enterica serovar Typhimurium (13.3%), and S. enterica subsp. enterica serovar Indiana (13.3%) were the four most common subspecies among the investigated 30 isolates. Detected virulence genes included pagN (100%), hilA (96.7%), ssrB (96.7%), prgH (86.7%), and marT (86.7%). The isolates exhibited resistance to nalidixic acid (43.3%) and kanamycin (43.3%), followed by streptomycin (16.7%) and ciprofloxacin (3.3%). Antibiotic-resistant genes were detected as follows: strA, strB, qnrA, qnrS, parC, aadA, aac(6′)-Ib, and aac(6′)-Ib-cr at 33.3%, 6.7%, 16.7, 13.3%, 10%, 23.3%, 6.7%, and 10%, respectively. The findings highlight the necessity of educational initiatives aimed at reducing reptile-related infections. Effective antibiotic treatment appears promising for infection, given the minimal drug resistance observed in reptile Salmonella serovars in the current study.

show abstract

Section: Identifcation Of Salmonella Species Using 16s Rrnamentioning

confidence: 99%

Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa

Mlangeni,

Ramatla,

Lekota

et al. 2024

International Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…This method has found its special place in meat microbiology, as a fast and inexpensive method, additionally characterised by high accuracy in the identification of bacteria. The identification of microorganisms is primarily based on the detection of ribosomal proteins, but also mitochondrial proteins that can be isolated [52][53][54][55].…”

Section: Microbiological Analysismentioning

confidence: 99%

The Effect of Temperature and Storage Duration on the Quality and Attributes of the Breast Meat of Hens after Their Laying Periods

Augustyńska-Prejsnar,

Hanus,

Ormian

et al. 2023

Foods

View full text Add to dashboard Cite

The purpose of this study was to evaluate the effect of temperature (2 °C and 6 °C) and storage duration on the quality and attributes of hens’ breast meat after their laying periods. The study included physicochemical characteristics (pH, drip loss, colour, shear force), microbiological quality (total Enterobacteriaceae family and Pseudomonas count), and sensory quality. Bacterial identification was performed using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. The increased meat pH and drip loss was greater at 6 than 2 °C (p < 0.05). An increase in the tenderness of the meat stored at 6 °C was found as early as day 4, as well as at 2 °C on day 8 of storage (p < 0.05). On day 4 of storage, the meat was characterised by a darker colour than on the first day, but the darkening was greater at 6 °C than at 2 °C (p < 0.05). At 6 °C, on day 4 of storage, there was an increase in yellow saturation (b*) of the meat, which was higher at 6 °C than at 2 °C (p < 0.05). At 2 °C, the total bacterial count and number of Pseudomonas spp. in the meat gradually increased along with increasing storage duration, reaching 4.64 log cfu/g and 4.48 log cfu/g, respectively, on the 8th day of storage. At 6 °C, on the sixth day of storage, the total bacterial count in the meat exceeded 7 log cfu/g, considered the limit of microbiological safety. The meat stored at 2 °C had an acceptable sensory quality until the 8th day of storage. The study shows that storage at 2 °C preserves the sensory characteristics and microbiological safety of the hen meat longer at an acceptable level after the laying period. Extended storage life may be of importance to consumers and the meat industry.

show abstract

“…Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) can be used to identify foodborne pathogens rapidly and sensitively (Crottaa et al, 2022). MALDI‐TOF‐MS is used as a fast, sensitive, and applicable method for the identification of pathogenic bacteria that cause diseases in ready‐to‐eat foods (Ramatla, Ngoma, & Mwanza, 2021; Teodoro, Carvalho, Queiroz, Levy, & Kabuki, 2022). In recent years, MALDI‐TOF‐MS has gained increasing importance in the field of food microbiology (Akimowicz & Bucka‐Kolendo, 2020; Taban Mercanoglu & Numanoglu Cevik, 2021).…”

Section: Introductionmentioning

confidence: 99%

Contribution of MALDI‐TOF‐MS‐based principal component analysis for distinguishing foodborne pathogens

Cevik

Kaynar

2023

Journal of Food Safety

View full text Add to dashboard Cite

Foodborne diseases are important to determine bacteria in strain level, which are analyzed by library‐based devices and bioinformatics‐enabled. The aim of this study was to investigate the contribution of principal component analysis (PCA) with matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) to distinguish according to the differences of bacterial strains as rapidly screening of foodborne bacteria. The MALDI‐TOF‐MS‐based PCA analysis was used for differentiating bacterial strains isolated from ready‐to‐eat foods. According to the results of PCA analysis, the percentages of distance and proximity between species were evaluated by composite correlation indexes (CCI). Bacillus cereus were detected in burghul salad (BC1) and macaroni salad (BC2) taken from the SB2‐snack bar, and the similarity rate was determined as 97%. Three other B. cereus bacteria (BC3, BC4, and BC5) in the same cluster were also isolated from salads collected from SB4‐snack bar. The similarity of Klebsiella pneumoniae bacteria, which have the codes KP1 and KP2, isolated from macaroni salad and burghul salad taken from the SB2 snack bar respectively were 96%. Additionally, the CCI values of two E. coli strains in burghul (EC1) salad and Russian salad (EC2) in the same sampling point (SB1) were determined as 97%. In conclusion, this analysis with MALDI‐TOF‐MS based PCA has revealed the relationships between bacteria genera and species, beyond just the identification of bacteria and the rapid screening of bacteria in perishable foodstuffs. Similarities between bacterial strains identified for different samples from the same sampling point suggested that there were not adequate hygiene rules and storage requirements were not followed.

show abstract

The Utility of MALDI-TOF-Mass Spectrometry, Analytical Profile Index (API) and Conventional-PCR for the Detection of Foodborne Pathogens from Meat

Cited by 5 publications

References 4 publications

Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa

Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa

The Effect of Temperature and Storage Duration on the Quality and Attributes of the Breast Meat of Hens after Their Laying Periods

Contribution of MALDI‐TOF‐MS‐based principal component analysis for distinguishing foodborne pathogens

Contact Info

Product

Resources

About