The Utilization of Carbon Compounds by Some
            <i>Actinomycetales</i>
            as an Aid for Species Determination

Pridham, T. G.; Gottlieb, David

doi:10.1128/jb.56.1.107-114.1948

Cited by 692 publications

(177 citation statements)

References 4 publications

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“…Strains belonging to different colour 'series' or 'groups' currently recognized within the genus Streptomyces ( A c t i m y c e s * ) were included. Among the reasons considered when selecting cultures for the present study was their ability to grow on Pridham & Gottlieb's (1948) synthetic medium supplemented with D-fructose as a sole carbon and energy source. Some of the strains included in a preliminary screening (Nikitina & Kalakoutskii, 197 1) failed to give secondary, variant colonies.…”

Section: Methodsmentioning

confidence: 99%

Actinomycete Variants with Impaired Differentiation: Production in High Yield, Recognition and Phenotypic Characterization in Seven Species

Nikitina¹,

Kalakoutskii

1973

Journal of Applied Bacteriology

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When plated on mineral synthetic media with D-fructose as 8 sole carbon and energy source 7 strains of actinomycetes belonging to different streptomycete species regularly produced secondary variant colonies in high yield. Beside the ability of streptomycetes to utilize fructose as a sole carbon source, the following factors were shown to be of importance in the control of variational events: the state of the parent culture used for platings; the composition of fructose-containing media used for the production of variants. Indole, anthranilate and phenol inhibited formation of secondary colonies by most strains. Phenotypically all the variants obtained shared in common the loss of ability t o form secondary (aerial) mycelium and spores as well as a tendency of the substrate mycelium to fragment. These traits were shown t o be: hereditarily stable on all media tested for variants derived from Streptomyces ruber, Str. roseolus, Str. lateritiw and Str. roseoJawus var. roseofungini ; less stable and nutritionally affected for variants derived from Str. albocyaneua, Str. r o s e o f i w and Str. antlwcyanew; unstable on a11 except fructose-containing mineral medium for the variants of Str.jZawojungini. Vegetative growth of some of the variants obtained was not inferior to that of parent cuItures; some variants produced increased amounts of intracelldtw antibiotics. Some implications of the reported &dings are discussed.

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Section: Methodsmentioning

confidence: 99%

Actinomycete Variants with Impaired Differentiation: Production in High Yield, Recognition and Phenotypic Characterization in Seven Species

Nikitina¹,

Kalakoutskii

1973

Journal of Applied Bacteriology

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“…-* Growth of the strain measured as the dry weight of the mycelium '+++' -good growth; '++' -moderate growth; '+'weak growth; '-'indicates negative/no growth. Utilization of carbon sources by the strains could be used as an aid for species determination 28 . The strain VJMS-18 efficiently utilized the carbon sources such as D-glucose, lactose, maltose, sucrose, galactose, sorbitol, mannitol and starch but could not utilize fructose, arabinose, cellulose and mannose Table 3.…”

Section: Biochemical Characteristics Of Vjms-18mentioning

confidence: 99%

Untitled

2019

IJPSR

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Objective: To evaluate the antimicrobial potential of Nocardiopsis flavescens isolated from the marine environment. Methods: Attempts were made to isolate actinomycete strains from marine coastal regions of Andhra Pradesh, India. The soil samples collected were pre-treated with calcium carbonate, diluted and plated on three different media to isolate actinomycetes. All the isolates were screened for antimicrobial activity by using the Kirby-Bauer disc diffusion method. Among the fifty isolates screened, one potent strain designated as VJMS-18 exhibited high antimicrobial activity. The isolate was identified by using micro-morphological, cultural, biochemical, physiological and molecular approaches. Results: The Potent strain was identified as Nocardiopsis flavescens VJMS-18. It showed good growth on YMD agar amended with 3% NaCl. Growth pattern of the isolate was studied by culturing it in YMD broth. The culture broth was extracted with ethyl acetate and tested for antimicrobial activity. It exhibited high antimicrobial activity against gram positive and gram negative bacteria as well as fungi. Conclusion: The present investigation reveals that Nocardiopsis flavescens VJMS-18 isolated from the coastal environment is the potential source of novel bioactive compounds.

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“…1B). The ability of strain NK660 to utilize various carbon sources was evaluated according to the method of Pridham and Gottlieb (1948). The carbon utilization test results are shown in Table 1.…”

Section: Isolation and Identification Of Strain Nk660mentioning

confidence: 99%

Cloning of ε‐poly‐L‐lysine (ε‐PL) synthetase gene from a newly isolated ε‐PL‐producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans

Geng

Yang

et al. 2014

Microbial Biotechnology

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ε-Poly-L-lysine (ε-PL), showing a wide range of antimicrobial activity, is now industrially produced as a food additive by a fermentation process. A new strain capable of producing ε-PL was isolated from a soil sample collected from Gutian, Fujian Province, China. Based on its morphological and biochemical features and phylogenetic similarity with 16S rRNA gene, the strain was identified as Streptomyces albulus and named NK660. The yield of ε-PL in 30 l fed-batch fermentation with pH control was 4.2 g l−1 when using glycerol as the carbon source. The structure of ε-PL was determined by nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS). Previous studies have shown that the antimicrobial activity of ε-PL is dependent on its molecular size. In this study, the polymerization degree of the ε-PL produced by strain NK660 ranged from 19 to 33 L-lysine monomers, with the main component consisting of 24–30 L-lysine monomers, which implied that the ε-PL might have higher antimicrobial activity. Furthermore, the ε-PL synthetase gene (pls) was cloned from strain NK660 by genome walking. The pls gene with its native promoter was heterologously expressed in Streptomyces lividans ZX7, and the recombinant strain was capable of synthesizing ε-PL. Here, we demonstrated for the first time heterologous expression of the pls gene in S. lividans. The heterologous expression of pls gene in S. lividans will open new avenues for elucidating the molecular mechanisms of ε-PL synthesis.

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The Utilization of Carbon Compounds by Some Actinomycetales as an Aid for Species Determination

Cited by 692 publications

References 4 publications

Actinomycete Variants with Impaired Differentiation: Production in High Yield, Recognition and Phenotypic Characterization in Seven Species

Actinomycete Variants with Impaired Differentiation: Production in High Yield, Recognition and Phenotypic Characterization in Seven Species

Untitled

Cloning of ε‐poly‐L‐lysine (ε‐PL) synthetase gene from a newly isolated ε‐PL‐producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans

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