Flower pedicels, more contaminated with moulds than cotton boll apices, contributed to the infection of the cellulose-rich locks. Similar to the total number of contaminants recovered from all boll segments, locks mycoflora was also significantly enhanced with boll age. Number of isolates from Gezira consistently conformed with changes in atmospheric relative humidity attaining a maximum in the 4th week following flower opening. Locks from the relatively dry and hot Shambat area exhibited an inconsistent mycofloral development although they collected 6.3% more infection than those picked from Gezira. An insignificant correlation between boll age and either pedicel or boll apex ifection was recorded. The relatively humid Gezira not only stimulated greater mould isolates (49.4% over Shambat's), but provided for 52.3% more infected boll apices over Shambat's.Twenty fungal genera and species, 90% of which were cellulolytic (2), were recovered from 1-7 week-old green bolls. Aspergillus nidulans and Cladosporium herbarum unintermittently persisted throughout the sampling duration. Abundance of these two contaminants insignificantly matched with either boll age or sampling area. Eurotium was first record to native lint.