The Utilization of Poly(2‐ethyl‐2‐oxazoline)‐b‐Poly(ε‐caprolactone) Ellipsoidal Particles for Intracellular BIKDDA Delivery to Prostate Cancer

Abstract

Prostate cancer is the most common cancer, which is about 15–20% among male cancers worldwide. As most common strategies such as radiotherapy, chemotherapy, or surgery alone can be unsuccessful in the treatment of prostate cancer, this study aims to develop a new approach to deliver newly generated proapoptotic gene, BIKDDA, to androgen independent prostate cancer cells, 22RV1, using new generation nanocarriers called ellipsoids. As far as it is known, this is the first study that assesses the ability of proapoptotic gene BIKDDA to induce apoptosis in prostate cancer cell. BIKDDA encapsulating PEtOx‐b‐PCL‐based ellipsoids are fabricated by solvent‐switch method, and their morphology, size, and BIKDDA content are characterized. Gene delivery efficiency of BIKDDA loaded PEtOx‐b‐PCL ellipsoids is demonstrated by analysis of BIK mRNA expression with real‐time PCR. The apoptotic effect of PEtOx‐b‐PCL ellipsoids loaded with BIKDDA (EPs‐BIKDDA) on 22RV1 is shown by Annexin V staining. The obtained results demonstrate that the treatment of 22RV1 cells with EPs‐BIKDDA can significantly increase BIK mRNA levels by 4.5‐fold leading to cell death. This study not only represents BIKDDA as a potential therapeutic strategy in prostate cancer but also the capacity of ellipsoids as promising in vivo gene delivery vehicles.

Development of peptide‐18‐targeted nanoliposome formulations with an alternative stealth coating copolymer for targeting breast cancer AU565 cell line

“…PEtOx‐N 3 (M n,GPC = 5800 g/mol, Ɖ = 1.05) was prepared similar to the previous works by living cationic ring‐opening polymerization of 2‐ethyl‐2‐oxazoline 7–9 . Briefly, a solution of mesyl functional initiator (0.35 g, 1.25 mmol) and dry 2‐ethyl 2‐oxazoline (5 mL, 49.5 mmol) in 15 ml dry acetonitrile were placed in a dried Schlenk tube equipped with a magnetic stir bar.…”

“…6 PEtOx-N 3 (M n,GPC = 5800 g/mol, Ɖ = 1.05) was prepared similar to the previous works by living cationic ring-opening polymerization of 2-ethyl-2-oxazoline. [7][8][9] Briefly, a solution of mesyl functional initiator (0.35 g, 1.25 mmol) and dry 2-ethyl 2-oxazoline (5 mL, 49.5 mmol) in 15 ml dry acetonitrile were placed in a dried Schlenk tube equipped with a magnetic stir bar. The polymerization was continued overnight under nitrogen atmosphere at 130 C. The reaction mixture was arranged at 65 C, and a 5-fold excess of NaN 3 was added to terminate for 3 days.…”

“…PEtOx-based copolymers showed no significant toxicity on human epithelial kidney (HEK293), endothelial cells (HUVEC), or mesenchymal stem cells (MSC). 8,9 In our previous study, we synthesized poly(2-ethyl-2-oxazoline)-based copolymers by click reactions and reported that these copolymers showed no toxicity on human epithelial breast cell (MCF10A) and two different breast cancer (AU565 and MDA-MB-231) cell lines. 6 Furthermore, PEtOx-DOPE liposome constructs displayed a low toxicity profile on HEK293, HUVEC, MSCs, and MCF10A, suggesting that they may serve as nanocarriers for drugs.…”

Amfoteri̇si̇n-B Enkapsüle Edi̇lmi̇ş Nanoparti̇külleri̇n Anti̇mi̇krobi̇yal Potensi̇ni̇n Değerlendi̇ri̇lmesi̇ İçi̇n Farmakope Yöntemi̇ni̇n Uygulanmasi

“…Polymeric based particles have the ability to encapsulate medicinal molecules within their core or matrix, or to attach them to their surface. In order to enhance their interaction with biological systems, polymeric nanoparticles may be made with a variety of forms, sizes, charges, and surface features [1]. Polymeric nanoparticles can also be modified with functional groups or ligands to improve their targeting, controlled release, or stimulus sensitivity.…”