The vaginal microbiota in the course of bacterial vaginosis treatment

Zwittink, Romy D.; Munckhof, Ellen H A van den; Hall, Maurine A. Leverstein–van; Boers, Kim E.; Molijn, Anco; Knetsch, Cornelis W.; Kuijper, Ed J.

doi:10.1007/s10096-020-04049-6

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…Several studies have indicated that bacterial vaginosis is associated with infertility [11]. Bacterial vaginosis has also been shown to alter the vaginal microbiome [12]. A previous study demonstrated that the vaginal microbiome plays an important role in the pathophysiology of primary ovarian failure, and the relative abundance of bacteria in the genus Lactobacillus was significantly lower in women with primary ovarian failure than in healthy controls [13].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the vaginal microbiome in women with premature ovarian insufficiency

Ning

Tan

et al. 2021

J Ovarian Res

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Purpose To investigate the relationship between vaginal microbial community structure and premature ovarian insufficiency (POI). Methods Twenty-eight women with POI and 12 healthy women were recruited at Shenzhen Maternity and Child Healthcare Hospital between August and September 2020. Blood samples were collected for glucose tests and detection of sex hormone levels and vaginal secretions were collected for microbial group determination. Vaginal microbial community profiles were analysed by 16S rRNA gene sequencing using the Illumina MiSeq system (Illumina Inc., San Diego, CA, USA). Results Compared to the controls, the serum levels of follicle-stimulating hormone, luteinizing hormone, testosterone, and the follicle-stimulating hormone/luteinizing hormone ratio, significantly increased, and oestradiol and anti-Müllerian hormone levels significantly decreased in women with POI. Higher weighted UniFrac values were observed in women with POI than in healthy women. Bacteria in the genera Lactobacillus, Brevundimonas, and Odoribacter were more abundant in the microbiomes of healthy women, while the quantity of bacteria in the genus Streptococcus was significantly increased in the microbiomes of women with POI. Moreover, these differences in microbes in women with POI were closely related to follicle-stimulating hormone, luteinizing hormone, oestradiol, and anti-Müllerian hormone levels and to the follicle-stimulating hormone/luteinizing hormone ratio. Conclusions Women with POI had altered vaginal microbial profiles compared to healthy controls. The alterations in their microbiomes were associated with serum hormone levels. These results will improve our understanding of the vaginal microbial community structure in women with POI. Trial registration CHICTR, ChiCTR2000029576. Registered 3 August 2020 - Retrospectively registered, https://www.chictr.org.cn/showproj.aspx?proj=48844.

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Section: Introductionmentioning

confidence: 99%

Characteristics of the vaginal microbiome in women with premature ovarian insufficiency

Ning

Tan

et al. 2021

J Ovarian Res

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“…Our data suggest that the current definition of BV, based on microscopy, is inadequate and will need revision as further genomic based studies improve our understanding of the condition. 27 Our study has a number of limitations. The study population was limited to women with recurrent and symptomatic BV and participants were mostly sexual health attendees, therefore our findings may not be generalisable to asymptomatic women or women with less severe BV.…”

Section: Discussionmentioning

confidence: 93%

The association between symptom or microscopy based diagnosis of bacterial vaginosis (BV) and response to treatment in women with recurrent BV

Ayinde¹,

Ross²

2023

Int J STD AIDS

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Background Bacterial vaginosis (BV) is a common condition, yet accurate diagnosis remains a challenge. We explored symptom and microscopy-based diagnosis of BV and assessed the influence of these diagnostic approaches on treatment response. Methods BV diagnosis based on patient-reported symptoms, and vaginal swab gram stain microscopy at local laboratories and a central laboratory were compared for women recruited into the VITA trial in England. Multivariable analysis was used to assess the association between the method of diagnosis and symptom resolution 2 weeks after metronidazole treatment. Results 517 women presenting with vaginal discharge (470/517 [91%]) and/or malodour (440/517 [85%]) were included. The accuracy of patients’ vaginal symptoms compared to local laboratory microscopy for BV diagnosis were —discharge, sensitivity 90% and specificity 5%; malodour, sensitivity 84% and specificity 12%, and compared to a central laboratory diagnosis were —discharge, sensitivity 91% and specificity 8%; malodour, sensitivity 88% and specificity 18%. 143/204 (70%) participants reported symptom resolution after treatment and this was associated with a positive baseline local laboratory diagnosis (adjusted relative risk—aRR 1.64 [1.02 to 2.64]), but not with a positive central laboratory diagnosis (aRR 1.14 [0.95 to 1.37]). Symptom resolution occurred in 75% (83/111) of women who were symptom positive/central laboratory BV positive compared to 65% (58/89) who were symptom positive/microscopy negative. Conclusion Symptoms correlated poorly with microscopy-based BV diagnosis but two-thirds of women with a symptom positive/microscopy negative diagnosis experienced symptom resolution following metronidazole treatment. Further studies are needed to determine the optimal investigation and treatment for microscopy-negative women with typical BV symptoms.

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“…In the event of confirmed anaerobes, the next step is usually antibiotic therapy with metronidazole (oral or vaginal) or clindamycin (oral or vaginal) [ 77 ]. One reason for the high rate of re-infection is the different spectrums of activity of metronidazole and clindamycin: Gardnerella vaginalis and Atopobium vaginae are sensitive to clindamycin, but the simultaneous activity of clindamycin against lactobacilli is disadvantageous.…”

Section: Antibioticsmentioning

confidence: 99%

Vaginal Microbiome in Reproductive Medicine

et al. 2022

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The human microbiome has been given increasing importance in recent years. The establishment of sequencing-based technology has made it possible to identify a large number of bacterial species that were previously beyond the scope of culture-based technologies. Just as microbiome diagnostics has emerged as a major point of focus in science, reproductive medicine has developed into a subject of avid interest, particularly with regard to causal research and treatment options for implantation failure. Thus, the vaginal microbiome is discussed as a factor influencing infertility and a promising target for treatment options. The present review provides an overview of current research concerning the impact of the vaginal microbiome on the outcome of reproductive measures. A non-Lactobacillus-dominated microbiome was shown to be associated with dysbiosis, possibly even bacterial vaginosis. This imbalance has a negative impact on implantation rates in assisted reproductive technologies and may also be responsible for habitual abortions. Screening of the microbiome in conjunction with antibiotic and/or probiotic treatment appears to be one way of improving pregnancy outcomes.

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The vaginal microbiota in the course of bacterial vaginosis treatment

Cited by 12 publications

References 25 publications

Characteristics of the vaginal microbiome in women with premature ovarian insufficiency

Characteristics of the vaginal microbiome in women with premature ovarian insufficiency

The association between symptom or microscopy based diagnosis of bacterial vaginosis (BV) and response to treatment in women with recurrent BV

Vaginal Microbiome in Reproductive Medicine

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