The validated pharmaceutical standard operating procedure and quality control study of the coded polyherbal tablet formulation AYUSH SG-5

Kumar, Vijay; Kushwaha, Vikram; Charde, Vaibhav; Jagtap, Chandrashekhar; Gandhi, Yashika; Grewal, Jyotika; Verma, Ravi; Rawat, Hemant; Mishra, Subhajit; Thakur, Akanksha; Babu, G. Raveendra; Singh, Arjun; Singh, Ravindra Kumar; Srikanth, Narayanam; Dhiman, Kartar Singh

doi:10.1016/j.sajb.2022.02.038

Cited by 12 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, there is a need to develop SOPs of each and every formulation and should follow these developed SOPs in the future. [6] Many natural products and their derivatives have been recognized as potent antimicrobial agents by the various researchers. In this present era, most of the microorganism developed resistance to many antibiotics which creating an immense clinical problem to treat the infectious disease.…”

Section: Original Articlementioning

confidence: 99%

Untitled

2022

IJGP

View full text Add to dashboard Cite

Introduction:Herbal medicines are being used since ancient times. Although herbal medications are considered safe, their authenticity and purity have been questioned. It is essential to establish relevant SOPs at the source to ensure the authenticity, purity, quality, and efficacy of herbal medications. Antibiotic resistance is developing every day and is now considered a severe health hazard all over the world, so new medications from natural sources are needed. Therefore, the objective of the study was to develop the SOPs and to evaluate the antimicrobial activity of Qurṣ-i Bel (QB). Materials and Methods: In the present study, 12 batches of QB were prepared to develop SOPs with Loabe Samaghe Arabi (mucilage of Acacia arabica) (GAM), a 100 mesh sieve, and various temperatures and drying times employed for each batch. The antimicrobial activity was done using the agar well diffusion method against four pathogenic bacteria, that is, Staphylococcus aureus, Escherichia coli, Klebsiella species, and Pseudomonas species. Results: The final batch (batch no.12), in which 15% w/w GAM used as a binding agent and dried at 90°C for 120 min showed hardness 17.66 ± 0.57-18.33 ± 1.15 kg/cm 2 , friability 0.0192 ± 0.00-0.0199 ± 0.00 %, and disintegration time 14.00 ± 0.00 min. The findings revealed that the methanolic and hydroalcoholic extracts of QB have antibacterial action against the three pathogens examined (Staphylococcus aureus, Escherichia coli, and Pseudomonas species). While, Klebsiella pneumoniae showed no zone of inhibition for all concentrations in all three extracts and aqueous extract was found ineffective against all tested organisms and showed no zone of inhibition. Conclusion: Its SOPs might be employed as traditional medicine for the treatment of various infections and could be used as a future reference for process standardization.

show abstract

Section: Original Articlementioning

confidence: 99%

Untitled

2022

IJGP

View full text Add to dashboard Cite

show abstract

“…The stem bark of SA is mentioned in various traditional drugs. [3] The leaves of SA are used as good source of tea in Europe. [2,4] Salix family including SA are known for salicylate-based phytochemicals including the salicin and isosalicin where salicylic acid is the basic precursor.…”

Section: Introductionmentioning

confidence: 99%

“…[2,7] The analytical studies on SA are very limited including capillary electrophoretic, [8] solid phase extraction cum high performance thin layer chromatography (SPE-HPTLC) [9] and high-performance liquid chromatography (HPLC). [2,3] As per our best knowledge, there is no report on simultaneous quantification of phytochemicals including Salicin, Procyanidin B and Catechin in different parts of SA including heart wood, stem bark and leaves. Hereby, a HPLC method was developed alongwith analysis of metal ions (by ICP-OES), volatile metabolites (by GC-MS/MS) and invitro antioxidant, antacid, and antimicrobial activities.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Analysis of Phytochemicals, Metal Ions, Volatile Metabolites in Heart Wood, Stem Bark and Leaves of Salix alba L. along with in Vitro Antioxidant, Antacid, Antimicrobial Activities for Sake of Environment Conservation by Substitution of Stem Bark With Leaf

Gandhi,

Rawat,

Singh Dhanjal

et al. 2023

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

There is need to search substitution of stem bark with leaf of Salix alba L, which is yet not reported. Comparative phytochemicals viz. Salicin, Procyanidin B1 and Catechin were quantified in the heart wood (SA‐HW), stem bark (SA‐SB) and leaves (SA‐L) of Salix alba L.by using newly developed HPLC method. Essential metal ions of all three parts were analysed using newly developed ICP‐OES method, where SA‐L were founded as rich source of micronutrients and essential metal ions as compared to SA‐SB and SA‐HW. GC‐MS/MS analysis has shown the presence of fatty acids and volatile compounds. The observed TPC and TFC values for all three parts were ranged from 2.69 to 32.30 mg GAE/ g of wt. and 37.57 to 220.76 mg QCE/ g of wt. respectively. In DPPH assay the IC50 values of SA‐SB, SA‐HW, and SA‐L were 1.09 (± 0.02), 5.42 (± 0.08), and 8.82 (± 0.10) mg/mL, respectively. The order of antibacterial activities against E. coli, S. aureus, P. aeruginosa, and B. subtilis strains was SA‐L > SA‐HW > SA‐SB with strong antibacterial activities against S. aureus, and B. subtilis strains. The antacid activities order was SA‐L > SA‐SB > SA‐HW.

show abstract

“…It is a well-known fact that many plants are the basis of modern medicine. Well-known drug molecules like taxol, aspirin, and morphine originate from plants or the natural system [ 1 – 9 ]. As per the World Health Organization (WHO) reports, for primary healthcare, more than 70% of the world population relies on natural resources [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…As per the World Health Organization (WHO) reports, for primary healthcare, more than 70% of the world population relies on natural resources [ 10 , 11 ]. Recent studies report the important role of traditional medicines in combating coronavirus disease 2019 (COVID–19) [ 1 ]. To certify the safety of consumers and to gain their faith, all the drugs, either herbal or modern, need standardization [ 8 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Combination of high-performance thin-layer chromatography and liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry analysis: a promising analytical tool for discrimination between oleo-gum resin of raw and purified Commiphora wightii

Charde

Jagtap

Gandhi

et al. 2022

JPC-J Planar Chromat

Self Cite

View full text Add to dashboard Cite

Guggulu ( Commiphora wightii oleo-gum resin) is exported in more than 42 countries including developed countries like the United Kingdom and the United States of America. The demand for guggulu is more than its production. Various purification processes have been mentioned in Ayurvedic classical literature which are not explored well yet, so it is impossible to discriminate between purified and raw guggulu. In the present study, an effort was made where guggulu was purified by using cow urine and changes were analyzed by using high-performance thin-layer chromatography (HPTLC) and liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry (LC–QTOF–MS/MS). In HPTLC analysis, three new bands ( R F 0.31, 0.68, 0.74) and twelve other bands were observed including the bands of marker compounds guggulsterone E ( R F 0.81) and Z ( R F 0.85) at 254 nm. In LC–QTOF–MS/MS analysis, two new peaks at Rt 5.00 and 16.21 min were observed in cow urine-purified guggulu. After purification, all the peaks were resolute well which shows the effects of purification. Overall, in LC–MS/MS study, metabolites, viz ., 5-(13′ Z -nonadecenyl)resorcinol (Rt 8.31), 8 β -hydroxy-3,20-dioxopregn-4,6-diene (Rt 10.33), guggulsterone E (Rt 12.99), guggulsterone Z (Rt 13.28), guggulsterol I (Rt = 15.19), mangiferolic acid (Rt = 15.27), 20( S ),21-epoxy-3-oxocholest-4-ene (Rt = 15.75), and guggulsterol II (Rt 16.23) were identified. The present study reports the first time a comparative analysis of raw guggulu and cow urine-purified guggulu by using advanced analytical tools like HPTLC and LC–QTOF–MS/MS. This study may be helpful in the standardization of and quality control of raw guggulu and cow urine-purified guggulu used in various formulations of guggulu.

show abstract

The validated pharmaceutical standard operating procedure and quality control study of the coded polyherbal tablet formulation AYUSH SG-5

Cited by 12 publications

References 18 publications

Untitled

Untitled

A Comparative Analysis of Phytochemicals, Metal Ions, Volatile Metabolites in Heart Wood, Stem Bark and Leaves of Salix alba L. along with in Vitro Antioxidant, Antacid, Antimicrobial Activities for Sake of Environment Conservation by Substitution of Stem Bark With Leaf

Combination of high-performance thin-layer chromatography and liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry analysis: a promising analytical tool for discrimination between oleo-gum resin of raw and purified Commiphora wightii

Contact Info

Product

Resources

About