The Validity of theAspergillus nidulansLinkage Map

Clutterbuck, A. J.

doi:10.1006/fgbi.1997.0984

Cited by 39 publications

(29 citation statements)

References 45 publications

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“…Interpretation of binary hybridization matrices, statistical and other mathematical aspects related to the construction, and signal evaluation of the physical maps have been described in detail elsewhere (Cuticchia et al, 1992;Wang et al, 1994a,b;Xiong et al, 1996). The revised genetic maps were obtained from the University of Glasgow Server (Clutterbuck, 1997).…”

Section: Methodsmentioning

confidence: 99%

“…Loci that appear in both maps are shown in the center and anchor physical contigs to the genetic map. The genetic map shows only loci that make up the map backbone and is from Clutterbuck (1997). Loci involved in physical/genetic anchoring or establishment of mapping distances are marked in bold.…”

Section: Figmentioning

confidence: 99%

“…The contig map is shown on the left side and the partial list of genetically mapped loci on the right (Clutterbuck, 1997). Loci listed in the middle were used to align (link) both maps.…”

Section: Graphic Integration Of Genetic (Linkage) and Physical (Cosmimentioning

confidence: 99%

“…The physical (contig) map describing the 31-Mb Aspergillus nidulans genome has been completed , and a comprehensive revision of the linkage (genetic) map has also recently been reported (Clutterbuck, 1997). The clone (cosmid) map was deduced solely on interpretation of a binary DNA/DNA hybridization matrix produced by nonoverlapping probes and overlapping clones (Cuticchia et al, 1992;Wang et al, 1994a,b;Prade et al, 1997).…”

mentioning

confidence: 99%

“…One difference is that physical maps built based on the overlap between clones are highly precise across short distances and become less accurate over longer distances because the probability of a false join increases with distance (Lander and Waterman, 1988;Arratia et al, 1991). Thus, the A. nidulans physical (clone) map is as reliable as is its weakest link (Clutterbuck, 1997).…”

mentioning

confidence: 99%

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The Reliability of the Aspergillus nidulans Physical Map

Prade

2000

Fungal Genetics and Biology

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Section: Methodsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

“…The contig map is shown on the left side and the partial list of genetically mapped loci on the right (Clutterbuck, 1997). Loci listed in the middle were used to align (link) both maps.…”

Section: Graphic Integration Of Genetic (Linkage) and Physical (Cosmimentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Reliability of the Aspergillus nidulans Physical Map

Prade

2000

Fungal Genetics and Biology

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Editorial

Arnold¹

1997

Fungal Genetics and Biology

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What is fungal genomics? Fungal genomics is the study of the structure, function, and evolution of fungal genomes. What separates this subject from traditional genetics is the study of 1000 or more genes or genetic markers simultaneously. As a consequence, specialized instrumentation is involved in generating genomic data, and the large amounts of genomic information require the use of sophisticated mathematical tools for building a coherent picture of a genome. These data are then made available to communities of scientists with novel database tools. This information has many applications in science, industry, agriculture, and medicine, leading to a number of bioethical issues surrounding the uses of this public information. The new experimental and technological approaches of genomics are thus inherently multidisciplinary, involving the contributions of individuals in molecular biology, biological instrumentation, computational biology, databases, statistics, and bioethics.Fungi have played a key role in the development of genomics. Their compact genomes have been exploited to develop new genomic tools, like yeast artificial chromosomes (Burke et al., 1987) and novel physical mapping algorithms (Cuticchia et al., 1992), for larger eukaryotic genomes. The ability to carry out site-directed transformation places fungi in a unique position relative to plants and animals for functional studies of many genes at once, the subject of functional genomics. For example, a library of strains containing gene knockouts is being assembled for the budding yeast, Saccharomyces cerevisiae (Goffeau et al., 1996). In the past, only fungi with well developed genetic tools could yield information efficiently-now genomic approaches allow genetic information to be gleaned from even the most intractable organisms, obligates, anaerobes, symbionts, and asexuals. The ability to use pulsed field electrophoresis (Cushion et al., 1993) to separate fungal chromosomes even in unculturable mammalian pathogens, like Pneumocystis carinii, allows a single investigator to create a physical map with markers evey 29 kb for such a pathogen in a single granting cycle, thereby converting an intractable system into a ''model system.'' Fungal genetics is entering a renaissance because it is now feasible to study simultaneous expression of all genes in a fungal genome (Schena et al., 1995) in a variety of fundamental biological processes, like meiosis, recombination, development, metabolism, virulence, and evolution. The 50 years of physiology, biochemistry, and genetics on model systems, like Neurospora crassa (Radford and Parrish, 1997) and Aspergillus nidulans , provide functional information on up to 10% of the genes in the genome (Kupfer et al., 1997; Radford and Parrish, 1997). The result is that fungal genomics provides a Rosetta stone for understanding the function, biochemistry, and physiology of the organism, on one side, and its relationship to genome structure, the underlying genetic blueprint, on the other side.The focus of this special iss...

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Mitosis in Filamentous Fungi: How We Got Where We Are

Aist

Morris

1999

Fungal Genetics and Biology

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The Validity of theAspergillus nidulansLinkage Map

Cited by 39 publications

References 45 publications

The Reliability of the Aspergillus nidulans Physical Map

The Reliability of the Aspergillus nidulans Physical Map

Editorial

Mitosis in Filamentous Fungi: How We Got Where We Are

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