This article defines and quantifies important factors of the quality of the business environment for small and medium-sized firms (SMEs) in the Czech Republic.To do so, itcompares the attitudes of entrepreneurs categorized by gender, education, age and firm size. A study of the business environment was conducted in 2015 from a sample of 1,141 respondents (the owners of companies), and the results were analyzed. It was found that only 10% of the entrepreneurs positively evaluated the applicable forms of state financial support. The study also demonstrated no statistically-significant response differences among the designated social groups. 64% of Czech entrepreneurs feel the support of their surroundings while doing business,45% think that SMEs have restricted access to external sources of financing, and over half note the intensive influence of market risk. It was also determined that there are statistically-significant differences in the pattern of responses between men and women and between micro-enterprises and small and medium-sized enterprises.