The value of subendocardial haemorrhages as an indicator of exsanguination and brain injury – A retrospective forensic autopsy study

Plattner, Thomas; Yen, Kathrin; Zollinger, Ulrich

doi:10.1016/j.jflm.2007.12.019

Cited by 7 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Certain kinds of cardiac injury occur in severely ill patients that differ from acute coronary syndromes. For example, subendocardial haemorrhages are often observed after blood loss or brain injury [ 45 ]. As most of the severe cardiac injuries accompany histopathological changes, we excluded such cases from the control cases in this study.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Stability in Postmortem FFPE Tissues: Quantitative Analysis Using Autoptic Samples from Acute Myocardial Infarction Patients

et al. 2015

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are very short (18–24 nucleotides) nucleic acids that are expressed in a number of biological tissues and have been shown to be more resistant to extreme temperatures and pH compared to longer RNA molecules, like mRNAs. As miRNAs contribute to diverse biological process and respond to various kinds of cellular stress, their utility as diagnostic biomarkers and/or therapeutic targets has recently been explored. Here, we have evaluated the usefulness of miRNA quantification during postmortem examination of cardiac tissue from acute myocardial infarction (AMI) patients. Cardiac tissue was collected within one week of the patient’s death and either frozen (19 samples) or fixed in formalin for up to three years (36 samples). RNA integrity was evaluated with an electropherogram, and it appears that longer RNAs are fragmented after death in the long-term fixed samples. Quantitative PCR was also performed for seven miRNAs and three other small RNAs in order to determine the appropriate controls for our postmortem analysis. Our data indicate that miR-191 and miR-26b are more suitable than the other types of small RNA molecules as they are stably detected after death and long-term fixation. Further, we also applied our quantitation method, using these endogenous controls, to evaluate the expression of three previously identified miRNA biomarkers, miR-1, miR-208b, and miR-499a, in formalin-fixed tissues from AMI patients. Although miR-1 and miR-208b decreased (1.4-fold) and increased (1.2-fold), respectively, in the AMI samples compared to the controls, the significance of these changes was limited by our sample size. In contrast, the relative level of miR-499a was significantly decreased in the AMI samples (2.1-fold). This study highlights the stability of miRNAs after death and long-term fixation, validating their use as reliable biomarkers for AMI during postmortem examination.

show abstract

Section: Discussionmentioning

confidence: 99%

MicroRNA Stability in Postmortem FFPE Tissues: Quantitative Analysis Using Autoptic Samples from Acute Myocardial Infarction Patients

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Furthermore, the autopsy provided additional findings consistent with exsanguination that were not detected on imaging. The condition of the right kidney and the subendocardial hemorrhages were additional signs of exsanguination, 10,11 aside from the sparsely developed livor mortis and the large amount of blood in the abdomen cavity.…”

Section: Discussionmentioning

confidence: 99%

Postmortem Computed Tomography and Magnetic Resonance Imaging of an Abdominal Gunshot Wound

Gascho

Bolliger

Thali

et al. 2020

Am J Forensic Med Pathol

View full text Add to dashboard Cite

The use of postmortem computed tomography (CT) has been described in many articles concerning gunshot injuries. Postmortem magnetic resonance imaging (MRI) for better assessment of soft tissue injuries has also been mentioned in the literature, albeit much less often. The use of postmortem MRI for abdominal gunshot wounds has not been previously presented in the literature. The present case report describes the findings of an abdominal gunshot wound detected by postmortem CT and MRI, followed by an autopsy. The main imaging findings on CT were a hyperdense ring at the entrance wound, which indicated the muzzle imprint mark, a hyperdense region beneath the skin, which was suggestive of combustion residue, gas cavities surrounding the bullet path, which might be related to the temporary cavity, and a fracture of the 13th rib on the left. Magnetic resonance imaging provided a clear depiction of defects in the muscle tissue and peritoneal fat, as well as an injury to the left kidney and a large volume of blood in the abdominal cavity. Computed tomography combined with MRI provided a descriptive presentation of the intracorporeal trajectory noninvasively. Autopsy confirmed the radiologic findings but additionally revealed further relevant findings, which were not detected radiologically, such as a duodenal perforation. Autopsy also detected subendocardial hemorrhages and shock kidney, which were consistent with severe blood loss.The imaging findings and their interpretations are discussed in this case report, as well as the role of CT and MRI in the assessment of abdominal gunshot wounds compared with autopsy.

show abstract

“…Die Toxine der pathogenen Bakterien führten zu ausgeprägten Nekrosen der Mukosaepithelien und nachfolgend zu einer erheblichen Blutung in das Darmlumen, die das Tier nicht überlebte. Die Befunde, die diese Erklärung stützen, sind die großen Blutmengen im Zäkum und im Colon descendens sowie die subendokardialen Blutungen des linken Ventrikels, die bei der Todesursache Verbluten relativ häufig festgestellt werden [19].…”

Section: Enterocolitis As the Cause Of Sudden Unexpected Death In A Cunclassified

Enterokolitis als Ursache eines akuten unerwarteten Todes eines Orang-Utans in Zoohaltung

et al. 2020

View full text Add to dashboard Cite

ZusammenfassungEs wird von einem 13 Jahre alt gewordenen weiblichen Orang-Utan berichtet, der 4 Tage vor dem Tod an einer akuten Enterokolitis erkrankte. Die tierärztliche Ultraschalluntersuchung einen Tag vor dem Tod erbrachte den Nachweis von vermehrter Flüssigkeit im Zäkum und einer abschnittsweise verdickten Kolonwand. Nach dem für die Veterinärmediziner und die Zoomitarbeiter unerwarteten Todeseintritt erfolgte eine Obduktion durch Rechtsmediziner der Universitätsmedizin Rostock mit Unterstützung von Ärzten weiterer Fachrichtungen. Dabei wurde als Todesursache eine akute Escherichia-coli-Enterokolitis mit massiver Blutung in den Darm festgestellt. Begünstigend oder ursächlich für die letale Enterokolitis dürften wahrscheinlich zahlreiche rezidivierende Durchfallerkrankungen in der Anamnese des Orang-Utans als Folge eines Verweigerns des Stillens durch die Mutter nach der Geburt gewesen sein.

show abstract

The value of subendocardial haemorrhages as an indicator of exsanguination and brain injury – A retrospective forensic autopsy study

Cited by 7 publications

References 7 publications

MicroRNA Stability in Postmortem FFPE Tissues: Quantitative Analysis Using Autoptic Samples from Acute Myocardial Infarction Patients

MicroRNA Stability in Postmortem FFPE Tissues: Quantitative Analysis Using Autoptic Samples from Acute Myocardial Infarction Patients

Postmortem Computed Tomography and Magnetic Resonance Imaging of an Abdominal Gunshot Wound

Enterokolitis als Ursache eines akuten unerwarteten Todes eines Orang-Utans in Zoohaltung

Contact Info

Product

Resources

About