The Value of Topleaf Inoculation to Demonstrate Genetic Resistance in Pomoideae Species to Erwinia Amylovora (Burr.) Winslow Et Al.

Geesteranus, H.P. Maas; Heyting, J.

doi:10.17660/actahortic.1981.117.12

Cited by 3 publications

(2 citation statements)

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“…Although trees are infected by E . amylovora primarily through the flowers under natural conditions in the field [30, 31], resistance phenotyping in the genetic mapping studies listed above were performed by artificial inoculation of grafted scions in the glasshouse, using either the cut-leaf [32, 33] or the hypodermic needle technique [34]. In studies comparing the resistance of shoots and flowers, Thibault and Le Lezec [35] reported a weak correlation between them, while Maroofi and Mostafavi [36] found a positive association.…”

Section: Introductionmentioning

confidence: 99%

Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation

et al. 2019

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Background: Although the most common path of infection for fire blight, a severe bacterial disease on apple, is via host plant flowers, quantitative trait loci (QTLs) for fire blight resistance to date have exclusively been mapped following shoot inoculation. It is not known whether the same mechanism underlies flower and shoot resistance. Results: We report the detection of a fire blight resistance QTL following independent artificial inoculation of flowers and shoots on two F1 segregating populations derived from crossing resistant Malus ×robusta 5 (Mr5) with susceptible 'Idared' and 'Royal Gala' in experimental orchards in Germany and New Zealand, respectively. QTL mapping of phenotypic datasets from artificial flower inoculation of the 'Idared' × Mr5 population with Erwinia amylovora over several years, and of the 'Royal Gala' × Mr5 population in a single year, revealed a single major QTL controlling floral fire blight resistance on linkage group 3 (LG3) of Mr5. This QTL corresponds to the QTL on LG3 reported previously for the 'Idared' × Mr5 and an 'M9' × Mr5 population following shoot inoculation in the glasshouse. Interval mapping of phenotypic data from shoot inoculations of subsets from both flower resistance populations reconfirmed that the resistance QTL is in the same position on LG3 of Mr5 as that for flower inoculation. These results provide strong evidence that fire blight resistance in Mr5 is controlled by a major QTL on LG3, independently of the mode of infection, rootstock and environment. Conclusions: This study demonstrates for the first time that resistance to fire blight caused by Erwinia amylovora is independent of the mode of inoculation at least in Malus ×robusta 5.

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Section: Introductionmentioning

confidence: 99%

Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation

et al. 2019

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“…Prior to inoculation, these trees were held in the greenhouse under a temperature regime of 25°C (day) and 20°C (night), with 80% relative humidity (RH) for 14 days. The plants were then acclimated to 26°C and 95% RH for inoculation by the cut-leaf method [ 50 ]. Actively growing shoots about 25 cm long were inoculated by cutting off 2/3 of the two youngest expanding leaves with scissors dipped in an aqueous buffer suspension of E. amylovora strain Ea236 at 1 x 10 9 cfu/mL.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of intergeneric hybrids between Malus and Pyrus

Pasqualetto

Palmieri

Martens

et al. 2022

Horticulture Research

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Apple (Malus) and pear (Pyrus) are economically important fruit crops well known for their unique textures, flavours, and nutritional qualities. Both genera are characterised by a distinct pattern of secondary metabolites, which directly affect not only resistance to certain diseases, but also have significant impacts on the flavour and nutritional value of the fruit. The identical chromosome numbers, similar genome size, and their recent divergence date, together with DNA markers have shown that apple and pear genomes are highly co-linear. This study utilized comparative genomic approaches, including simple sequence repeats, high resolution single nucleotide polymorphism melting analysis, and single nucleotide polymorphism chip analysis to identify genetic differences among hybrids of Malus and Pyrus, and F2 offspring. This research has demonstrated and validated that these three marker types, along with metabolomics analysis are very powerful tools to detect and confirm hybridity of progeny derived from crosses between apple and pear in both cross directions. Furthermore, this work analysed the genus-specific metabolite patterns and the resistance to fire blight (Erwinia amylovora) in progeny. The findings of this work will enhance and accelerate the breeding of novel tree fruit crops that benefit producers and consumers, by enabling marker assisted selection of desired traits introgressed between pear and apple.

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A QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds

et al. 2016

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The Value of Topleaf Inoculation to Demonstrate Genetic Resistance in Pomoideae Species to Erwinia Amylovora (Burr.) Winslow Et Al.

Cited by 3 publications

References 0 publications

Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation

Mapping of fire blight resistance in Malus ×robusta 5 flowers following artificial inoculation

Molecular characterization of intergeneric hybrids between Malus and Pyrus

A QTL detected in an interspecific pear population confers stable fire blight resistance across different environments and genetic backgrounds

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