The value of ΔG° for the hydrolysis of ATP

Kinetic limitations in the overall reaction of mitochondrial oxidative phosphorylation accounting for flux‐dependent changes in the apparent ΔGexP/ΔH+ ratio

“…For determination of extramitochondrial adenine nucleotides and G-6-P, I ml samples were withdrawn from the incubation mixture and quickly filtered through glass fibre filters (Millipore Type AP 25) for separation of the mitochondria into 4 ml of a phenol/chloroform/isoamyl alcohol mixture (38:24:1) (v/v) saturated with water plus 0.6 ml of 66 mM EDTA for quenching and extraction [2]. Extramitochondrial phosphorylation potentials were calculated using a computer program [3] taking into account the standard phosphorylation potentials published in [4]. The rates of phosphorylation (Jp) were estimated from the G-6-P formation.…”

Phosphorylation without protonmotive force

“…The concentrations of ADP and ATP were calculated from the ADP and Pi present at zero time and the Pi removed from the medium. The AGs for the synthesis of ATP were calculated from these concentrations using the appropriate AG o of Rosing and Slater [24].…”

The influence of thyroid hormone on the degree of control of oxidative phosphorylation exerted by the adenine nucleotide translocator

“…This implies that at 1 mM total adenine nucleotide, the ADP concentration at state 3 may be about 165 ,uM. Under the conditions of this experiment (5 mM Mg2+, 2 mM EDTA, 15 mM K+) most of the ADP would be complexed with Mg2+ [25,26]. Thus, near state 3, the concentration of magnesium-ADP complexes is well above the apparent K,,, of the translocator for this substrate in either euthyroid or hypothyroid liver [27,10].…”