The Variability of Translocation Yields Amongst Control Populations

Sorokine-Durm, I.; Whitehouse, Caroline; Edwards, Alan

doi:10.1093/oxfordjournals.rpd.a033026

Cited by 32 publications

(12 citation statements)

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“…Gender did not affect TF, in agreement with several previous studies conducted among control populations [23,24]. We were unable to distinguish the separate effects of laboratory and race, especially with respect to differences between TFs among Asians compared to Blacks.…”

Section: Discussionsupporting

confidence: 87%

“…Nearly all studies have reported age as a strong predictor of TFs [11,12,15,[23][24][25] but upward curvature at older ages was not consistently observed. We definitively demonstrate the presence of upward curvature at older ages in this report.…”

Section: Discussionmentioning

confidence: 99%

“…Pooled analyses of translocation data in unexposed individuals using fluorescence in situ hybridization (FISH) whole chromosome painting have been conducted among 436 and 385 persons [23,24]. In these and other studies, background translocation frequencies were strongly influenced by age with a suggestion of an upward curvature at older ages [10][11][12][23][24][25]. However, whether the translocation and age relationship was modified by gender, race, or smoking has remained unclear.…”

Section: Introductionmentioning

confidence: 99%

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International study of factors affecting human chromosome translocations

Sigurdson

Hauptmann

et al. 2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

122

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Abstract*Correspondence, page proofs, and reprint requests to: James D. Tucker, Ph.D., Department of Biological Sciences, 1370 Biological Sciences Building, 5047 Gullen Mall, Wayne State University, Detroit, TEL: (313) 577-2783, FAX: (313) 577-3602, EMAIL: E-mail: jtucker@biology.biosci.wayne.edu. z Retired Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Chromosome translocations in peripheral blood lymphocytes of normal, healthy humans increase with age, but the effects of gender, race, and cigarette smoking on background translocation yields have not been examined systematically. Further, the shape of the relationship between age and translocation frequency (TF) has not been definitively determined. We collected existing data from sixteen laboratories in North America, Europe, and Asia on TFs measured in peripheral blood lymphocytes by fluorescence in situ hybridization whole chromosome painting among 1933 individuals. In Poisson regression models, age, ranging from newborns (cord blood) to 85 years, was strongly associated with TF and this relationship showed significant upward curvature at older ages vs. a linear relationship (p <0.001). Ever smokers had significantly higher TFs than non-smokers (rate ratio (RR) = 1.19, 95% confidence interval (CI), 1.09-1.30) and smoking modified the effect of age on TFs with a steeper age-related increase among ever smokers compared to non-smokers (p<0.001). TFs did not differ by gender. Interpreting an independent effect of race was difficult owing to laboratory variation. Our study is three times larger than any pooled effort to date, confirming a suspected curvilinear relationship of TF with age. The significant effect of cigarette smoking has not been observed with previous pooled studies of TF in humans. Our data provide stable estimates of background TF by age, gender, race, and smoking status and suggest an acceleration of chromosome damage above age 60 and among those with a history of smoking cigarettes. NIH Public Access

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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International study of factors affecting human chromosome translocations

Sigurdson

Hauptmann

et al. 2008

Mutation Research/Genetic Toxicology and Environmental Mutagene

122

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“…Ces études peuvent être regroupées en deux groupes : celles regroupant les données issues de plusieurs laboratoires pour avoir plus de poids statistique (Sigurdson et al, 2008 ;Sorokine-Durm et al, 2000b ;Whitehouse et al, 2005), et celles qui présentent les données d'un seul laboratoire (Darroudi et Natarajan, 2000 ;Pressl et al, 1999 ;Ramsey et al, 1995) (Tab. II).…”

Section: Effet De L'âgeunclassified

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

et al. 2010

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The assessment of exposure to ionizing radiation, carried out long time after exposure, is currently performed by scoring of translocations, a specific type of chromosomal aberrations. The translocations rate observed in peripheral blood lymphocytes of exposed subjects is compared to that observed in a control population. However, the translocation specificity towards radiation exposure is not clearly identified. To avoid any hasty conclusion, it is necessary to identify all the factors likely to induce translocation. To our knowledge, no study has thus far examined the effects of all these different factors on translocation rates. A review of the literature thus allowed us to assess the impact of host factors and lifestyle on the production of translocations. This study confirms that age appears to be the factor 1 IRSN, Laboratoire de Dosimétrie Biologique, BP 17, 92262 Fontenay-aux-Roses Cedex, France.Article publié par EDP Sciences E. GRÉGOIRE et al. 154 RADIOPROTECTION -VOL. 45 -N° 2 (2010) having the greatest impact on the rate of translocations, especially over 60 years. To date, the factor "age" is already considered in estimating the impact of radiation on the rate of translocation for all age groups. However, the study also shows that this rate varies significantly when the patient is exposed simultaneously and significantly towards many lifestyle agents. A precise threshold translocation rate should thus be established as a function of known behavioral exposures, below which it is impossible to conclude that radiological exposure has occurred. The effects of chemicals on the translocation rate after occupational exposure will be the subject of a second part.

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“…However, the use of PAINT translocations has been viewed as problematic because this system makes no mechanistic assumptions as to which abnormal chromosomes are formed as part of the same exchange configuration [Finnon et al, 1995]. Some investigators have suggested that only reciprocal (two-way) translocations should be scored as these are likely to be more stable over time than nonreciprocal (one-way) translocations [Lindholm and Salomaa, 2000;Littlefield et al, 2000;Moquet et al, 2000;Sorokine-Durm et al, 2000;Pala et al, 2001]. While there is evidence to support this assertion in humans [Gardner and Tucker, 2002] and mice [Spruill et al, 2000], scoring only reciprocal translocations while excluding other translocation types is also problematic.…”

Section: Introductionmentioning

confidence: 99%

Persistence of chromosome aberrations following acute radiation: II, Does it matter how translocations are scored?

Tucker

Cofield

Matsumoto³

et al. 2005

Environ and Mol Mutagen

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Chromosome breaks and rearrangements resulting from ionizing radiation can be much more complicated than many investigators thought possible some years ago. The realization that not all translocations are reciprocal, that multiway exchanges occur, and that some double-strand breaks are not repaired prior to mitosis have all contributed to the difficulty of knowing how best to identify, record, evaluate, and report chromosome translocations. Here we describe the results of a series of experiments in which blood from two normal healthy subjects was obtained, irradiated with 137Cs gamma-rays in vitro at doses ranging from 0 (controls) to 4 Gy, and cultured. Cells from each dose group and donor were harvested at days 2, 2.5, 3, 4, 5, and 7 and evaluated for chromosome damage by simultaneously painting chromosomes 1, 2, and 4 in red and 3, 5, and 6 in green. The persistence of dicentrics, fragments, rings, insertions, and PAINT translocations are reported separately by us in this issue. In this article, we focus on translocations, characterizing the various types in detail and comparing and contrasting their persistence across all dose groups for both donors. The results indicate that the persistence of all translocation types was sufficient to be used for retrospective dosimetry, although nonreciprocal translocations exhibited diminished persistence compared to the other types. We also characterize the kinetics of the radiation dose responses of the two donors who exhibited significant differences in the induction as well as the persistence of translocations. Based on the evidence presented here, we hypothesize that these individuals differ in the recognition and repair of radiation-induced damage as well as in cell cycle checkpoint control. Despite these differences, the temporal frequency of translocation losses at both the high and low doses was similar for both subjects.

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The Variability of Translocation Yields Amongst Control Populations

Cited by 32 publications

References 0 publications

International study of factors affecting human chromosome translocations

International study of factors affecting human chromosome translocations

Impact des facteurs individuels et environnementaux sur le taux d’aberrations chromosomiques de type translocations Partie 1 : âge, sexe, tabac, alcool

Persistence of chromosome aberrations following acute radiation: II, Does it matter how translocations are scored?

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