The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

Pévet, Paul; Neacsu, C; Holder, F.C.; Reinharz, A.; Dogterom, J.; Buijs, Ruud M.; Guerné, J.M.; Vivien-Rœls, B.

doi:10.1007/bf01248960

Cited by 13 publications

(1 citation statement)

References 16 publications

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“…Following the original discovery of a compound in aqueous extracts of bovine pineals with antidiuretic and hydroosmotic properties, considered to be a basic peptide with a disulphide linkage [Milcu et al, 19631, numerous confirmatory reports have appeared on the existence of an AVT-like bioactivity in the mammalian pineal [Pavel, 1971;Benson et al, 1976;Pevet et al, 1981;Larsen et al, 19821. The present paper describes the localization and partial characterization of a neurohormone-like bioactivity in an aqueous extract of ovine pineals.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Neurohypophyseal Hormone‐Like Activity Isolated From Ovine Pineal Glands

Noteborn

Ebels²,

Reinharz³

et al. 1988

Journal of Pineal Research

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The milk-ejecting response of lactating mouse mammary gland tissue to ovine pineal extracts indicated the presence of a neurohormone-like bioactivity in this tissue. After successive fractionation on gel permeation chromatography and reversed-phase liquid chromatography (HPLC) in conjunction with radioimmunoassays (RIA), it was demonstrated that the milk-ejection response to ovine pineal components with an Mr less than 1,000 corresponded to a biologically active peptide sequence that probably differs from that of arginine vasopressin, arginine vasotocin, and oxytocin and from peptides with a COOH-terminal Pro-Arg-Gly-amide ending. Gel permeation chromatography in formic acid appeared also to indicate the presence of a noncovalent interaction of the neurohormone-like bioactivity with proteins (Mr greater than 25,000) of the pineal.

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Section: Discussionmentioning

confidence: 99%

Characterization of a Neurohypophyseal Hormone‐Like Activity Isolated From Ovine Pineal Glands

Noteborn

Ebels²,

Reinharz³

et al. 1988

Journal of Pineal Research

Self Cite

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The Different Classes of Proteic and Peptidic Substances Present in the Pineal Gland

Pévet

1983

The Pineal Gland and Its Endocrine Role

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Modified forms of vasopressin and oxytocin in a bovine pineal preparation

1987

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A bovine pineal acid extract displays a vasotocin-like bioactivity in several bioassays, and is recognized by antibodies against the Pro-Arg-Gly-amide ending common to vasopressin and vasotocin. By using molecular sieve filtration and reversed-phase HPLC, a vasopressin-and oxytocin-like peptide was isolated from this pineal preparation, while no evidence for a vasotocin-like peptide was obtained. The isolated neuropeptides contain a modified amino acid at position 2. This structural difference with authentic pituitary vasopressin and oxytocin may alter their biological and immunological properties, which have been interpreted as vasotocin-like, and thus underlies the controversy concerning the existence of vasotocin in the mammalian pineal gland.

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The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

Cited by 13 publications

References 16 publications

Characterization of a Neurohypophyseal Hormone‐Like Activity Isolated From Ovine Pineal Glands

Characterization of a Neurohypophyseal Hormone‐Like Activity Isolated From Ovine Pineal Glands

The Different Classes of Proteic and Peptidic Substances Present in the Pineal Gland

Modified forms of vasopressin and oxytocin in a bovine pineal preparation

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