The ventrolateral periaqueductal gray is involved in the cardiovascular response evoked by l-glutamate microinjection into the lateral hypothalamus of anesthetized rats

Deolindo, Milena Vieira; Pelosi, Gislaine Garcia; Tavares, Rodrigo Fiacadori; Corrêa, Fernando Morgan de Aguiar

doi:10.1016/j.neulet.2007.10.028

Cited by 16 publications

(21 citation statements)

References 27 publications

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“…A volume of 100 nl was used because previous studies have calculated that microinjecting this volume into the brainstem could spread as far as ~1 mm 3 (Lipski et al , 1988; Mitra et al , 1993). In fact, microinjecting this volume (100 nl) into the raphe (Nucci et al , 2008) and hypothalamus (Deolindo et al , 2008) of rats has been used by other investigators recently. DAMGO microinjections were made outside of the cMRR and mMRR in two rats to further confirm the site-dependence of the evoked responses to the DAMGO microinjection into the cMRR and mMRR.…”

Section: Methodsmentioning

confidence: 99%

Opioid μ-receptors in medullary raphe region affect the hypoxic ventilation in anesthetized rats

Zhang

et al. 2009

Respiratory Physiology & Neurobiology

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Opioids can attenuate the peripheral chemoreceptor-mediated hypoxic ventilatory response (HVR) by acting on central μ-type opioid receptors. Since the medullary raphe region (MRRs) expresses abundant μ-receptors and participates in modulating HVR, we tested the role of μ-receptors within the caudal, medial, and rostral MRR (cMRR, mMRR, and rMRR) in modulating the HVR. We recorded cardiorespiratory activities and their responses to isocapnic hypoxia in anesthetized rats before and after local microinjection of DAMGO into the MRR, and intravenous administration of DAMGO (100 μg/kg) alone or coupled with a previous local injection of CTAP. Microinjecting DAMGO into the cMRR or mMRR but not the rMRR significantly attenuated the HVR. However, systemic DAMGO-induced HVR attenuation was not significantly affected by pretreating the cMRR and mMRR with CTAP. Our data suggest that cMRR and mMRR μ-receptors are capable of depressing the HVR, while their contribution to the attenuated HVR by systemic DAMGO is limited.

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Section: Methodsmentioning

confidence: 99%

Opioid μ-receptors in medullary raphe region affect the hypoxic ventilation in anesthetized rats

Zhang

et al. 2009

Respiratory Physiology & Neurobiology

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“…Previous studies reported an involvement of LH glutamatergic neurotransmission in cardiovascular control. It was shown that microinjection of l ‐glutamate into the LH of anaesthetized or conscious rats evokes a decrease in blood pressure that is accompanied by a decrease in heart rate (HR) in anaesthetized animals 25–27 . Although these results suggest an important role for LH glutamatergic neurotransmission in cardiovascular modulation, its involvement in the modulation of baroreflex activity has been less well studied.…”

Section: Introductionmentioning

confidence: 99%

Non‐N‐methyl‐d‐aspartate glutamate receptors in the lateral hypothalamus modulate cardiac baroreflex responses in conscious rats

Crestani

Deolindo

Alves

et al. 2009

Clin Exp Pharma Physio

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Summary 1. In the present study, we investigated the effects of inhibition of the lateral hypothalamus (LH) neurotransmission with bilateral microinjection of CoCl(2), a non-selective blocker of neurotransmission, on modulation of cardiac baroreflex responses in conscious rats as well as the involvement of LH glutamatergic neurotransmission in this modulation. 2. Reflex bradycardiac and tachycardiac responses to blood pressure increases (following i.v. infusion of phenylephrine) or decreases (following i.v. infusion of sodium nitroprusside) were investigated in conscious male Wistar rats. Responses were evaluated before and after microinjection of 1 nmol/100 nL CoCl(2), 2 nmol/100 nL 1,2,3,4-tetrahydro-6-nitro-2,3-dioxobenzoquinoxaline-7-sulphonamide (NBQX; a selective non-N-methyl-d-aspartate (NMDA) glutamate receptor antagonist) or different doses (2, 4 or 8 nmol/100 nL) of the selective NMDA glutamate receptor antagonist LY235959. 3. Microinjection of CoCl(2) into the LH had no effect on the tachycardiac baroreflex response, but did evoke a decrease in the reflex bradycardia caused by increases in blood pressure. Microinjection of NBQX into the LH had a similar effect on reflex bradycardia as CoCl(2), but had no effect on the tachycardiac response. Microinjection of increasing doses of LY235959 into the LH had no effect on the cardiac baroreflex response. 4. In conclusion, the data suggest that the LH has a tonic facilitatory influence on the parasympathetic component of the baroreflex. The results also indicate that this facilitatory influence is mediated by local LH glutamatergic neurotransmission through non-NMDA glutamatergic receptors.

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“…Cobalt is an inorganic calcium channel blocking agent used to suppress Ca 2+ -induced synaptic transmission (Kretz 1984). Microinjection of cobalt chloride (CoCl 2 ) has been used for the reversible inactivation of brain nuclei, in order to understand their functional role (Deolindo et al 2008;Ciriello et al 2008;Holden and Pizzi 2008;Pelosi et al 2007;Cavun and Millington 2001;Boscan and Paton 2001;Fisk and Wyss 2000). Cobalt is an inorganic calcium channel blocker used to suppress Ca 2+ -induced synaptic transmission (Kretz 1984).…”

Section: Discussionmentioning

confidence: 99%

“…The technique is based on giving restricted microinjections of compounds that reversibly block neuronal activity over a given period. Microinjections of CoCl 2 (cobalt) into discrete brain areas have been used for reversible functional inactivation (Kretz 1984;Deolindo et al 2008;Ciriello et al 2008;Pelosi et al 2007;Fisk and Wyss 2000). In addition, neuronal connections among brain structures may be studied by histological analysis of neuronal tracer distribution after their microinjection into selected brain areas.…”

Section: Introductionmentioning

confidence: 99%

The diagonal band of Broca is involved in the pressor pathway activated by noradrenaline microinjected into the periaqueductal gray area of rats

2009

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The ventrolateral periaqueductal gray is involved in the cardiovascular response evoked by l-glutamate microinjection into the lateral hypothalamus of anesthetized rats

Cited by 16 publications

References 27 publications

Opioid μ-receptors in medullary raphe region affect the hypoxic ventilation in anesthetized rats

Opioid μ-receptors in medullary raphe region affect the hypoxic ventilation in anesthetized rats

Non‐N‐methyl‐d‐aspartate glutamate receptors in the lateral hypothalamus modulate cardiac baroreflex responses in conscious rats

The diagonal band of Broca is involved in the pressor pathway activated by noradrenaline microinjected into the periaqueductal gray area of rats

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