2019
DOI: 10.1101/630236
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The Vertebrate Codex Gene Breaking Protein Trap Library For Genomic Discovery and Disease Modeling Applications

Abstract: 2The zebrafish is a powerful model to explore the molecular genetics and expression of the vertebrate 3 genome. The gene break transposon (GBT) is a unique insertional mutagen that reports the expression of 4 the tagged member of the proteome while generating Cre-revertible genetic alleles. This 1000+ locus 5 collection represents novel codex expression data from the illuminated mRFP protein trap, with 36% 6 and 87% of the cloned lines showcasing to our knowledge the first described expression of these genes 7… Show more

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Cited by 2 publications
(1 citation statement)
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“…The UFlip vector was designed to be compatible with our previously described short homology-based CRISPR/Cas9 knock in strategy, the GeneWeld (Wierson, Welker, Almeida et al, in press at eLife). The UFlip backbone and the gene trap cassette were adapted from a gene-break transposon vector (Clark et al, 2012;Ichino et al, 2019). The knock in cassette has two functional units: (1) a gene trap and (2) a secondary reporter.…”
Section: Author Contributionsmentioning
confidence: 99%
“…The UFlip vector was designed to be compatible with our previously described short homology-based CRISPR/Cas9 knock in strategy, the GeneWeld (Wierson, Welker, Almeida et al, in press at eLife). The UFlip backbone and the gene trap cassette were adapted from a gene-break transposon vector (Clark et al, 2012;Ichino et al, 2019). The knock in cassette has two functional units: (1) a gene trap and (2) a secondary reporter.…”
Section: Author Contributionsmentioning
confidence: 99%