The viability of sperm in lateral oviducts and spermathecae of instrumentally inseminated and naturally mated honey bee (<i>Apis mellifera</i> L.) queens

Gençer, H. Vasfi; Kahya, Yasin

doi:10.3896/ibra.1.50.3.02

Cited by 11 publications

(14 citation statements)

References 21 publications

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“…We determined 88.7 % viable spermatozoa in the lateral oviducts of the queens as soon as they returned from mating. This was similar to the viability of spermatozoa in the lateral oviducts of instrumentally inseminated queens (88.6 %) reported by Gençer and Kahya (2011b). According to these findings, spermatozoa damage during semen collection procedure seems to be compensated by less pressure effect during injecting semen into lateral oviducts in instrumental insemination.…”

Section: Discussionsupporting

confidence: 86%

“…However, undoubtedly, after eversion of the endophallus, the pressure required to inject the subsequent semen into semen-filled oviducts must be higher (viability loss, 6.1 %) than to flow out on the surface of the endophallus (viability loss, 3.3 %) during natural eversion as well as during manually provoked eversion. Gençer and Kahya (2011b) reported that there was no significant difference between the viability of spermatozoa in the lateral oviducts of the queens 4 h after instrumental insemination (88.6 %) and 4 h after natural mating (87.6 %). They suggested that the viability of spermatozoa in the lateral oviducts of naturally mated and instrumentally inseminated queens might decrease with time while spermatozoa migrate into the spermatheca.…”

Section: Discussionmentioning

confidence: 98%

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Why the viability of spermatozoa diminishes in the honeybee (Apis mellifera) within short time during natural mating and preparation for instrumental insemination

Gençer¹,

Kahya²,

Woyke³

2014

Apidologie

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The viability of spermatozoa is a crucial parameter to appreciate semen quality and insemination potential of males both in natural mating and instrumental insemination. Here, we conducted a step-by-step investigation to address the questions why and at which step(s) the viability loss is occurring in spermatozoa of honeybees during natural mating and preparation for instrumental insemination. We detected the viability of spermatozoa in semen samples obtained from seminal vesicles and partly and fully everted endophalli of drones and in ejaculates collected into syringe tips, as well as the viability of spermatozoa in lateral oviducts of queens returning from the mating flight. A great diminish of spermatozoa viability (~10 %) was found in lateral oviducts of queens returning from mating flight (88.7 %) in comparison to viable spermatozoa in intact seminal vesicles of drones (98.1 %). Our results demonstrated that the decrease in spermatozoa viability occurs during the second stage of eversion of endophallus (viability loss, 3.3 %), and during injection of semen into the lateral oviducts of queens (viability loss, 6.1 %). The acting factor decreasing the viability of spermatozoa was the increased pressure occurring during the process of natural and instrumental insemination. drone / queen / Apis mellifera / eversion of endophallus / spermatozoa viability / instrumental insemination

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

Why the viability of spermatozoa diminishes in the honeybee (Apis mellifera) within short time during natural mating and preparation for instrumental insemination

Gençer¹,

Kahya²,

Woyke³

2014

Apidologie

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“…4). Because the quality of semen in the spermatheca is superior to that in the oviduct56 and because dead spermatozoa do not migrate to the spermatheca5257, the migration process may be considered as a mechanism of selecting the best spermatozoa for storage to compensate for lower sperm quality and to maintain the sustainability of the species. Our data indicate that this protective mechanism may be circumvented when fertilization is performed with altered semen from drones exposed to environmental pollutants, such as Fipronil, resulting in decreased semen numbers and mortality rate in the spermatheca.…”

Section: Resultsmentioning

confidence: 99%

Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Kairo¹,

Provost

Tchamitchian³

et al. 2016

Sci Rep

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A species that requires sexual reproduction but cannot reproduce is doomed to extinction. The important increasing loss of species emphasizes the ecological significance of elucidating the effects of environmental stressors, such as pesticides, on reproduction. Despite its special reproductive behavior, the honey bee was selected as a relevant and integrative environmental model because of its constant and diverse exposure to many stressors due to foraging activity. The widely used insecticide Fipronil, the use of which is controversial because of its adverse effects on honey bees, was chosen to expose captive drones in hives via syrup contaminated at 0.1 μg/L and gathered by foragers. Such environmental exposure led to decreased spermatozoa concentration and sperm viability coupled with an increased sperm metabolic rate, resulting in drone fertility impairment. Subsequently, unexposed queens inseminated with such sperm exhibited fewer spermatozoa with lower viability in their spermatheca, leaving no doubt about the detrimental consequences for the reproductive potential of queens, which are key for colony sustainability. These findings suggest that pesticides could contribute to declining honey bee populations through fertility impairment, as exemplified by Fipronil. More broadly, reproductive disorders should be taken into consideration when investigating the decline of other species.

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“…To perform this evaluation, we used different semen dilutions for a 100-fold magnification, being 0% dilution, 50% and 60% diluted in 0.9% saline (Figure 2). Gençer and Kahya (2011) reported that the mean spermatozoa viability in queen oviducts after insemination is 88.10%, demonstrating the importance and care with semen collection and storage, as there is loss throughout the process, until the arrival at the spermatheca. The same authors reported that spermatozoa collected correctly and not killed during the procedure have viability similar to that of spermatozoa in the seminal vesicles, which is 98.10%.…”

Section: Resultsmentioning

confidence: 99%

Drone production, semen viability and spermatozoa longevity of Africanized Apis mellifera

Halak¹,

Santos

Rossi

et al. 2020

Acta Sci. Anim. Sci.

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Characteristics correlated with beekeeping production, less influenced by the environment and that can be controlled by management techniques, can help in the selection of colonies with higher production capacity, aiming to improve breeding programs. This research was carried out to evaluate the production of Africanized Apis mellifera drones and the longevity of spermatozoa from different genetic groups when supplemented with protein or not. Two genetic groups were used: one selected for royal jelly production from the Africanized honeybee breeding program and another without genetic selection. In both groups, the number of drone brood and the quality of semen were evaluated every 30 days, for five months. Statistical analysis was performed using Bayesian Inference. Statistical difference was found for the drone production among the treatments, and colonies without genetic selection produced more males (187.80 ± 11.15) than the selected colonies (93.07 ± 8.88). The selected colonies for royal jelly production presented greater efficiency in the reproductive cycle of males, because they produced fewer drones than colonies without genetic selection, however, with 31% greater semen viability.

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The viability of sperm in lateral oviducts and spermathecae of instrumentally inseminated and naturally mated honey bee (Apis mellifera L.) queens

Cited by 11 publications

References 21 publications

Why the viability of spermatozoa diminishes in the honeybee (Apis mellifera) within short time during natural mating and preparation for instrumental insemination

Why the viability of spermatozoa diminishes in the honeybee (Apis mellifera) within short time during natural mating and preparation for instrumental insemination

Drone exposure to the systemic insecticide Fipronil indirectly impairs queen reproductive potential

Drone production, semen viability and spermatozoa longevity of Africanized Apis mellifera

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