The Viable but Non-Culturable (VBNC) State in Vibrio Species: Why Studying the VBNC State Now Is More Exciting than Ever

Wagley, Sariqa

doi:10.1007/978-3-031-22997-8_13

Cited by 3 publications

(1 citation statement)

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“…The in situ water temperature also dictates culture growth rates, in particular environmental Vv from cooler samples [184]. Although the VBNC state, metabolic survival and pathogenesis responses of these bacteria have been extensively reviewed elsewhere [26,[185][186][187], most current environmental monitoring types of studies still do not consider the impact of inclusion of culture-based methods on Vibrio abundance underestimates. Modeling based on data from most studies using partially culture-based methods described in Table 2, such as liquid culture-based (MPN-) or solid medium (DP-) culturing are at risk of underestimating Vv and Vp that are possibly pathogenic but resist growth on our current culture media.…”

Section: Future Outlooks Advancements and New Molecular Toolsmentioning

confidence: 99%

Tools to Enumerate and Predict Distribution Patterns of Environmental Vibrio vulnificus and Vibrio parahaemolyticus

Waidner,

Potdukhe

2023

Microorganisms

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Vibrio vulnificus (Vv) and Vibrio parahaemolyticus (Vp) are water- and foodborne bacteria that can cause several distinct human diseases, collectively called vibriosis. The success of oyster aquaculture is negatively impacted by high Vibrio abundances. Myriad environmental factors affect the distribution of pathogenic Vibrio, including temperature, salinity, eutrophication, extreme weather events, and plankton loads, including harmful algal blooms. In this paper, we synthesize the current understanding of ecological drivers of Vv and Vp and provide a summary of various tools used to enumerate Vv and Vp in a variety of environments and environmental samples. We also highlight the limitations and benefits of each of the measurement tools and propose example alternative tools for more specific enumeration of pathogenic Vv and Vp. Improvement of molecular methods can tighten better predictive models that are potentially important for mitigation in more controlled environments such as aquaculture.

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